Injectable hydrogels of enzyme-catalyzed cross-linked tyramine-modified gelatin for drug delivery
Yuanhan Tang
A , Junjie Ding A , Xun Zhou A , Xintao Ma A , Yi Zhao A , Qiyu Mu A , Zixu Huang A , Qian Tao A * , Fangjie Liu B * and Ling Wang C *
+ Author Affiliations
- Author Affiliations
A School of Chemistry and Materials Science, Ludong University, Yantai, 264025, China.
B School of Food Engineering, Ludong University, Yantai, 264025, China.
C College of Chemistry and Chemical Engineering, Center of Cosmetics, Qilu Normal University, Jinan 250200, China.
Handling Editor: Charlotte Williams
Australian Journal of Chemistry 76(2) 88-99 https://doi.org/10.1071/CH22188
Submitted: 27 August 2022 Accepted: 3 February 2023 Published: 28 February 2023
© 2023 The Author(s) (or their employer(s)). Published by CSIRO Publishing.
Abstract
Enzymatically catalyzed cross-linking is a hydrogel fabrication method that generally is considered to have lower cytotoxicity than traditional chemical cross-linking methods. In order to optimize the properties of injectable hydrogels and expand their applications, an enzyme-catalyzed cross-linked injectable hydrogel was designed. The tyramine-modified gelatin (G-T) was formed into a stable injectable hydrogel by the combination of horseradish peroxidase (HRP) and hydrogen peroxide (H2O2) catalysis. 1H NMR spectroscopy was used to demonstrate the successful modification of gelatin by tyramine. The surface morphology of the prepared hydrogels was characterized jointly by atomic force microscopy (AFM) and scanning electron microscopy (SEM). Rheological tests demonstrated the tunable mechanical strength, formation kinetics, shear thinning and good self-recovery properties of the hydrogels. In addition, the hydrogels can be formed into various shapes by injection. The hydrogel network structure is complex and interlaced, as such it is suitable to encapsulate drugs for controlled release. The drug release from the prepared hydrogels followed the Peppas–Sahlin model and belonged to Fickian diffusion. This study constructed injectable hydrogels through the enzyme-catalyzed cross-linking of modified gelatin and applied the hydrogels for drug release, which is expected to expand the application in biomedical fields.
Keywords: drug delivery, enzyme-catalyzed, gelatin, hydrogel rheology, injectable hydrogel, release kinetics, tyramine modification, self‐recovery.
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