Synthesis of hydrogels incorporating core–shell structured Fe3O4@ZIF-8 as bio-nanocomposite carriers for drug delivery
Pagasukon Mekrattanachai
A , Naruemon Setthaya A , Chakkresit Chindawong A , Bunlawee Yotnoi A , Wei Guo Song B and Chawan Manaspon C *
+ Author Affiliations
- Author Affiliations
A School of Chemistry, Faculty of Science, University of Phayao, 19 Moo 2, Maeka, Muang District, Phayao, 56000, Thailand.
B Beijing National Laboratory for Molecular Sciences, Laboratory of Molecular Nanostructures and Nanotechnology, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, China.
C Biomedical Engineering Institute, Chiang Mai University, 239 Huay Kaew Road, Muang District, Chiang Mai, 50200, Thailand.
Australian Journal of Chemistry 76(4) 201-208 https://doi.org/10.1071/CH22224
Submitted: 21 October 2022 Accepted: 25 May 2023 Published: 15 June 2023
© 2023 The Author(s) (or their employer(s)). Published by CSIRO Publishing.
Abstract
Due to its high porosity and excellent pH-sensitive breakdown, the zeolitic imidazolate framework-8 (ZIF-8) has been investigated as a drug delivery vehicle. To increase the magnetic property of ZIF-8 nanoparticles, Fe3O4 nanoparticles (Fe3O4 NPs) were encapsulated to form a core–shell structure (Fe3O4@ZIF-8). The core–shell particles were then incorporated into a hydrogel to increase biocompatibility. The Fe3O4@ZIF-8-incorporated hydrogel nanocomposite (Fe3O4@ZIF-8 hydrogel) was then studied for in vitro cytotoxicity for drug delivery applications. Transmission electron microscopy images confirmed the core–shell structure of the synthesized Fe3O4@ZIF-8 with a central Fe3O4 core and a shell of nano-sized ZIF-8. The specific surface area of the obtained Fe3O4@ZIF-8 was 821 m2 g–1 with a pore volume of 0.36 cm3 g–1. The effect of the prepared Fe3O4@ZIF-8 on cell viability (mouse fibroblast, L929 cell line) was investigated using an MTT (3-(4,5-dimethylthiazolyl-2-yl)-2,5-diphenyltetrazolium bromide) assay. The Fe3O4@ZIF-8 and pure ZIF-8 showed a 50% inhibitory concentration at 28.2 + 1.0 and 11.2 + 6.6 µg mL–1, respectively. A chitosan/pluronic F-127 hydrogel incorporating Fe3O4@ZIF-8 was successfully prepared and showed over 75% cell viability compared to the fresh culture medium. Overall results indicated the safety of using chitosan/pluronic F-127 vehicles containing Fe3O4@ZIF-8 as a carrier for drug delivery systems.
Keywords: biocompatibility, core-shell structure, drug delivery system, Fe3O4 particles, hydrogels, metal–organic frameworks, nanocomposite, ZIF-8.
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