The Effect of Charge on Melittin-Induced Changes in Membrane Structure and Morphology
Tzong-Hsien Lee A B , Kristopher Hall A B and Marie-Isabel Aguilar A C
+ Author Affiliations
- Author Affiliations
A Department of Biochemistry and Molecular Biology, Monash University, Clayton, Vic. 3800, Australia.
B These authors contributed equally to this work.
C Corresponding author. Email: mibel.aguilar@monash.edu
Australian Journal of Chemistry 73(3) 195-201 https://doi.org/10.1071/CH19500
Submitted: 4 October 2019 Accepted: 4 November 2019 Published: 20 December 2019
Abstract
The binding of melittin to a range of phospholipid bilayers was studied using dual polarisation interferometry and atomic force microscopy. The phospholipid model membranes included zwitterionic dimyristylphosphatidylcholine (DMPC), together with mixtures of DMPC/dimyristylphosphatidylglycerol (DMPG) and DMPC/DMPG/cholesterol. Melittin caused significant disruption on all bilayers, but differences in morphological changes during binding were different on each membrane. Overall, the results demonstrate that the process of membrane disruption follows distinct structural changes for different lipid mixtures irrespective of the strength of binding to the membrane surface.
References
[1] H. Raghuraman, A. Chattopadhyay, Biosci. Rep. 2007, 27, 189.| Crossref | GoogleScholarGoogle Scholar | 17139559PubMed |
[2] M. A. Sani, F. Separovic, Acc. Chem. Res. 2016, 49, 1130.
| Crossref | GoogleScholarGoogle Scholar | 27187572PubMed |
[3] K. Hall, T. H. Lee, M. I. Aguilar, J. Mol. Recognit. 2011, 24, 108.
| Crossref | GoogleScholarGoogle Scholar | 21194121PubMed |
[4] R. Smith, F. Separovic, T. J. Milne, A. Whittaker, F. M. Bennett, B. A. Cornell, A. Makriyannis, J. Mol. Biol. 1994, 241, 456.
| Crossref | GoogleScholarGoogle Scholar | 8064858PubMed |
[5] J. Hong, X. Lu, Z. Deng, S. Xiao, B. Yuan, K. Yang, Molecules 2019, 24, 1775.
| Crossref | GoogleScholarGoogle Scholar |
[6] H. Mozsolits, T. H. Lee, H. J. Wirth, P. Perlmutter, M. I. Aguilar, Biophys. J. 1999, 77, 1428.
| Crossref | GoogleScholarGoogle Scholar | 10465754PubMed |
[7] K. Hall, H. Mozsolits, M. I. Aguilar, Lett. Pept. Sci. 2003, 10, 475.
| Crossref | GoogleScholarGoogle Scholar |
[8] D. I. Fernandez, T.-H. Lee, M.-A. Sani, M.-I. Aguilar, F. Separovic, Biophys. J. 2013, 104, 1495.
| Crossref | GoogleScholarGoogle Scholar | 23561526PubMed |
[9] T. H. Lee, K. N. Hall, M. J. Swann, J. F. Popplewell, S. Unabia, Y. Park, K. S. Hahm, M. I. Aguilar, Biochim. Biophys. Acta 2010, 1798, 544.
| Crossref | GoogleScholarGoogle Scholar | 20100457PubMed |
[10] T. H. Lee, C. Heng, M. J. Swann, J. D. Gehman, F. Separovic, M. I. Aguilar, Biochim. Biophys. Acta 2010, 1798, 1977.
| Crossref | GoogleScholarGoogle Scholar | 20599687PubMed |
[11] D. I. Fernandez, T. H. Lee, M. A. Sani, M. I. Aguilar, F. Separovic, Biophys. J. 2013, 104, 1495.
| Crossref | GoogleScholarGoogle Scholar | 23561526PubMed |
[12] T. H. Lee, D. J. Hirst, K. Kulkarni, M. P. Del Borgo, M. I. Aguilar, Chem. Rev. 2018, 118, 5392.
| Crossref | GoogleScholarGoogle Scholar | 29793341PubMed |
[13] D. J. Hirst, T. H. Lee, M. J. Swann, M. I. Aguilar, Anal. Chem. 2013, 85, 9296.
| Crossref | GoogleScholarGoogle Scholar | 23998643PubMed |
[14] K. Hall, T. H. Lee, A. I. Mechler, M. J. Swann, M. I. Aguilar, Sci. Rep. 2014, 4, 5479.
| Crossref | GoogleScholarGoogle Scholar | 24969959PubMed |
[15] C. Steinem, H. J. Galla, A. Janshoff, Phys. Chem. Chem. Phys. 2000, 2, 4580.
| Crossref | GoogleScholarGoogle Scholar |
[16] M. A. Bodescu, F. Rosenkotter, J. Fritz, Soft Matter 2017, 13, 6845.
| Crossref | GoogleScholarGoogle Scholar | 28829104PubMed |
[17] J. Dufourcq, J. F. Faucon, G. Fourche, J. L. Dasseux, M. Le Maire, T. Gulik-Krzywicki, Biochim. Biophys. Acta 1986, 859, 33.
| Crossref | GoogleScholarGoogle Scholar | 3718985PubMed |
[18] M. Monette, M. R. Van Calsteren, M. Lafleur, Biochim. Biophys. Acta 1993, 1149, 319.
| Crossref | GoogleScholarGoogle Scholar | 8323950PubMed |
[19] T. Pott, J. C. Maillet, C. Abad, A. Campos, J. Dufourcq, E. J. Dufourc, Chem. Phys. Lipids 2001, 109, 209.
| Crossref | GoogleScholarGoogle Scholar | 11269939PubMed |
[20] L. Y. Chen, C. W. Cheng, J. J. Lin, W. Y. Chen, Anal. Biochem. 2007, 367, 49.
| Crossref | GoogleScholarGoogle Scholar | 17570332PubMed |
[21] M. Meincken, D. L. Holroyd, M. Rautenbach, Antimicrob. Agents Chemother. 2005, 49, 4085.
| Crossref | GoogleScholarGoogle Scholar | 16189084PubMed |
[22] M. C. Giocondi, D. Yamamoto, E. Lesniewska, P. E. Milhiet, T. Ando, C. Le Grimellec, Biochim. Biophys. Acta 2010, 1798, 703.
| Crossref | GoogleScholarGoogle Scholar | 19796628PubMed |
[23] B. Gumí-Audenis, L. Costa, F. Carla, F. Comin, F. Sanz, M. I. Giannotti, Membranes 2016, 6, 58.
| Crossref | GoogleScholarGoogle Scholar |
[24] L. J. Johnston, Langmuir 2007, 23, 5886.
| Crossref | GoogleScholarGoogle Scholar | 17428076PubMed |
[25] L. Picas, P. E. Milhiet, J. Hernandez-Borrell, Chem. Phys. Lipids 2012, 165, 845.
| Crossref | GoogleScholarGoogle Scholar | 23194897PubMed |
[26] J. E. Shaw, J. R. Alattia, J. E. Verity, G. G. Prive, C. M. Yip, J. Struct. Biol. 2006, 154, 42.
| Crossref | GoogleScholarGoogle Scholar | 16459101PubMed |
[27] J. E. Shaw, R. F. Epand, R. M. Epand, Z. Li, R. Bittman, C. M. Yip, Biophys. J. 2006, 90, 2170.
| Crossref | GoogleScholarGoogle Scholar | 16361347PubMed |
[28] S. Rex, Biophys. Chem. 1996, 58, 75.
| Crossref | GoogleScholarGoogle Scholar | 8679920PubMed |
[29] K. Matsuzaki, S. Yoneyama, K. Miyajima, Biophys. J. 1997, 73, 831.
| Crossref | GoogleScholarGoogle Scholar | 9251799PubMed |
[30] A. S. Ladokhin, M. E. Selsted, S. H. White, Biophys. J. 1997, 72, 1762.
| Crossref | GoogleScholarGoogle Scholar | 9083680PubMed |
[31] D. Giménez, O. L. Sanchez-Munoz, J. Salgado, Langmuir 2015, 31, 3146.
| Crossref | GoogleScholarGoogle Scholar | 25705986PubMed |
[32] L. Yang, T. A. Harroun, T. M. Weiss, L. Ding, H. W. Huang, Biophys. J. 2001, 81, 1475.
| Crossref | GoogleScholarGoogle Scholar | 11509361PubMed |
[33] A. Mashaghi, M. Swann, J. Popplewell, M. Textor, E. Reimhult, Anal. Chem. 2008, 80, 3666.
| Crossref | GoogleScholarGoogle Scholar | 18422336PubMed |
[34] G. H. Cross, A. Reeves, S. Brand, M. J. Swann, L. L. Peel, N. J. Freeman, J. R. Lu, J. Phys. D 2004, 37, 74.
| Crossref | GoogleScholarGoogle Scholar |
