Aptamer-Based Biosensing with a Cationic AIEgen*
Tracey Luu A D , Mengjie Liu A D , Yilong Chen B , Roozbeh Hushiarian A , Anthony Cass C , Ben Zhong Tang B and Yuning Hong A E
+ Author Affiliations
- Author Affiliations
A Department of Chemistry and Physics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Vic. 3086, Australia.
B Department of Chemistry, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China.
C Department of Chemistry, Molecular Sciences Research Hub, Imperial College London, White City Campus, London 212 0BZ, UK.
D These authors contributed equally to this work.
E Corresponding author. Email: y.hong@latrobe.edu.au
Australian Journal of Chemistry 72(8) 620-626 https://doi.org/10.1071/CH19238
Submitted: 29 May 2019 Accepted: 1 July 2019 Published: 23 July 2019
Abstract
Fabrication of low-cost biosensing platforms with high selectivity and sensitivity is important for constructing portable devices for personal health monitoring. Herein, we report a simple biosensing strategy based on the combination of a cationic AIEgen (aggregation-induced emission fluorogen), TPE-2+, with an aptamer for specific protein detection. The target protein can displace the dye molecules on the dye–aptamer complex, resulting in changes in the fluorescence signal. Selectivity towards different targets can be achieved by simply changing the aptamer sequence. The working mechanism is also investigated.
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