Using Carbon Quantum Dots as Selective Photoluminescent Probes for Protein Kinase Assay
Yanjun Ding A B D , Wenjing Kang C , Xianzhong Xiao A , Kangkai Wang A , Nian Wang A , HuaLi Zhang A , Minghui Yang C D and Guoli Shen B
+ Author Affiliations
- Author Affiliations
A Department of Pathophysiology, Xiangya School of Medicine, Central South University, Changsha 410008, China.
B State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, China.
C Department of Chemistry and Chemical Engineering, Central South University, Changsha 410008, China.
D Corresponding authors. Email: yangminghui@csu.edu.cn; dingyanjun@csu.edu.cn
Australian Journal of Chemistry 68(8) 1249-1254 https://doi.org/10.1071/CH14554
Submitted: 13 September 2014 Accepted: 20 December 2014 Published: 23 March 2015
Abstract
A simple and sensitive fluorescent assay is developed for the detection of protein kinase A (PKA) activity based on the selective aggregation of phosphorylated peptide–carbon quantum dots (CQDs) conjugates triggered by Zr4+ ions coordination. The peptide was conjugated onto CQDs through covalent coupling. Under optimized conditions, a linear relationship between the decreases in fluorescence intensity of peptide–CQDs and the PKA concentration in the range from 0.125 to 30 U mL–1 was observed. Moreover, the inhibition effect of H-89 on the activity of PKA was studied, and the results indicated that the proposed method showed potential applications in kinase inhibitor screening.
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