Reliability of leaf functional traits after delayed measurements
Dinesh Thakur A B , Lakhbeer Singh A and Amit Chawla
A B C
+ Author Affiliations
- Author Affiliations
A Council of Scientific and Industrial Research (CSIR)–Institute of Himalayan Bioresource Technology (IHBT), Palampur, Kangra, Himachal Pradesh, 176061, India.
B Academy of Scientific and Innovative Research (AcSIR), CSIR–IHBT, Palampur, India.
C Corresponding author. Email: amitchawla21@gmail.com
Australian Journal of Botany 68(2) 100-107 https://doi.org/10.1071/BT19166
Submitted: 24 October 2019 Accepted: 17 March 2020 Published: 12 May 2020
Abstract
In this study, the effect of temporary storage (at 4°C) on measurement of leaf traits was tested. We collected leaf samples from 25 species, which represented different functional types in the high altitude vegetation of western Himalaya, to measure leaf area (LA), leaf rehydration, specific leaf area (SLA) and leaf dry matter content (LDMC). Repeated trait measurements were performed for up to 7 days. We found that in all the species, LA increased in initial 24 h of rehydration and thereafter remained stable. Leaf rehydration was found to be sensitive to delayed measurements and changed significantly for up to 7 days. For SLA and LDMC, the effect of storage time was significant only for a few species. On the basis of our findings, we recommend that, for samples stored in dark at 4°C, LA, SLA and LDMC can reliably be estimated after a delay of up to 7 days. Further, these key leaf traits should be estimated only after 24 h of rehydration. Also, trait measurements after prolonged rehydration of leaves should be avoided. Outcomes of this study will be beneficial when a large number of samples are collected from locations far away from laboratory and temporary storage is necessitated before trait measurements.
Additional keywords: Himalaya, leaf area, leaf dry matter content, rehydration percentage, relative water content, specific leaf area, temporary storage.
References
Auger S, Shipley B (2013) Inter-specific and intra-specific trait variation along short environmental gradients in an old-growth temperate forest. Journal of Vegetation Science 24, 419–428.| Inter-specific and intra-specific trait variation along short environmental gradients in an old-growth temperate forest.Crossref | GoogleScholarGoogle Scholar |
Bates D, Mächler M, Bolker B, Walker S (2015) Fitting linear mixed-effects models using lme4. Journal of Statistical Software 67, 1–51.
| Fitting linear mixed-effects models using lme4.Crossref | GoogleScholarGoogle Scholar |
Díaz S, Kattge J, Cornelissen JHC, Wright IJ, Lavorel S, Dray S, Reu B, Kleyer M, Wirth C, Colin Prentice I, Garnier E, Bönisch G, Westoby M, Poorter H, Reich PB, Moles AT, Dickie J, Gillison AN, Zanne AE, Chave J, Joseph Wright S, Sheremet Ev SN, Jactel H, Baraloto C, Cerabolini B, Pierce S, Shipley B, Kirkup D, Casanoves F, Joswig JS, Günther A, Falczuk V, Rüger N, Mahecha MD, Gorné LD (2016) The global spectrum of plant form and function. Nature 529, 167–171.
| The global spectrum of plant form and function.Crossref | GoogleScholarGoogle Scholar | 26700811PubMed |
Garnier E, Shipley B, Roumet C, Laurent G (2001) A standardized protocol for the determination of specific leaf area and leaf dry matter content. Functional Ecology 15, 688–695.
| A standardized protocol for the determination of specific leaf area and leaf dry matter content.Crossref | GoogleScholarGoogle Scholar |
Garnier E, Vile D, Roumet C, Lavorel S, Grigulis K, Navas M-L, Lloret F (2019) Inter- and intra-specific trait shifts among sites differing in drought conditions at the north western edge of the Mediterranean Region. Flora 254, 147–160.
| Inter- and intra-specific trait shifts among sites differing in drought conditions at the north western edge of the Mediterranean Region.Crossref | GoogleScholarGoogle Scholar |
González L, González-Vilar M (2001) Determination of relative water content. In ‘Handbook of Plant Ecophysiology Techniques’. pp. 207–212. (Kluwer Academic Publishers: Dordrecht, Netherlands)
Hudson JMG, Henry GHR, Cornwell WK (2011) Taller and larger: shifts in Arctic tundra leaf traits after 16 years of experimental warming. Global Change Biology 17, 1013–1021.
| Taller and larger: shifts in Arctic tundra leaf traits after 16 years of experimental warming.Crossref | GoogleScholarGoogle Scholar |
Juneau KJ, Tarasoff CS (2012) Leaf area and water content changes after permanent and temporary storage. PLoS One 7, e42604
| Leaf area and water content changes after permanent and temporary storage.Crossref | GoogleScholarGoogle Scholar | 22880051PubMed |
Luo Y, Liu J, Tan S, Cadotte MW, Xu K, Gao L, Li D (2016) Trait variation and functional diversity maintenance of understory herbaceous species coexisting along an elevational gradient in Yulong Mountain, Southwest China. Plant Diversity 38, 303–311.
| Trait variation and functional diversity maintenance of understory herbaceous species coexisting along an elevational gradient in Yulong Mountain, Southwest China.Crossref | GoogleScholarGoogle Scholar | 30159482PubMed |
Milla R, Reich PB (2007) The scaling of leaf area and mass: the cost of light interception increases with leaf size. Proceedings of the Royal Society of London – B. Biological Sciences 274, 2109–2115.
| The scaling of leaf area and mass: the cost of light interception increases with leaf size.Crossref | GoogleScholarGoogle Scholar |
Millar BD (1966) Relative turgidity of leaves: temperature effects in measurement. Science 154, 512–513.
Niinemets U (1999) Components of leaf dry mass per area – thickness and density – alter leaf photosynthetic capacity in reverse directions in woody plants. New Phytologist 144, 35–47.
| Components of leaf dry mass per area – thickness and density – alter leaf photosynthetic capacity in reverse directions in woody plants.Crossref | GoogleScholarGoogle Scholar |
Niinemets Ü (2010) A review of light interception in plant stands from leaf to canopy in different plant functional types and in species with varying shade tolerance. Ecological Research 25, 693–714.
| A review of light interception in plant stands from leaf to canopy in different plant functional types and in species with varying shade tolerance.Crossref | GoogleScholarGoogle Scholar |
Niklas KJ, Cobb ED, Niinemets U, Reich PB, Sellin A, Shipley B, Wright IJ (2007) ‘Diminishing returns’ in the scaling of functional leaf traits across and within species groups. Proceedings of the National Academy of Sciences of the United States of America 104, 8891–8896.
| ‘Diminishing returns’ in the scaling of functional leaf traits across and within species groups.Crossref | GoogleScholarGoogle Scholar | 17502616PubMed |
Pan S, Liu C, Zhang W, Xu S, Wang N, Li Y, Gao J, Wang Y, Wang G (2013) The scaling relationships between leaf mass and leaf area of vascular plant species change with altitude. PLoS One 8, e76872
| The scaling relationships between leaf mass and leaf area of vascular plant species change with altitude.Crossref | GoogleScholarGoogle Scholar | 24358136PubMed |
Park E, Cho M, Ki CS (2009) Correct use of repeated measures analysis of variance. Korean Journal of Laboratory Medicine 29, 1–9.
| Correct use of repeated measures analysis of variance.Crossref | GoogleScholarGoogle Scholar | 19262072PubMed |
Pavlica K, Holman D, Thorpe R (1998) The manager as a practical author of learning. Career Development International 3, 300–307.
| The manager as a practical author of learning.Crossref | GoogleScholarGoogle Scholar |
Pérez-Harguindeguy N, Díaz S, Garnier E, Lavorel S, Poorter H, Jaureguiberry P, Bret-Harte MS, Cornwell WK, Craine JM, Gurvich DE, Urcelay C, Veneklaas EJ, Reich PB, Poorter L, Wright IJ, Ray P, Enrico L, Pausas JG, de Vos AC, Buchmann N, Funes G, Quétier F, Hodgson JG, Thompson K, Morgan HD, ter Steege H, van der Heijden MGA, Sack L, Blonder B, Poschlod P, Vaieretti MV, Conti G, Staver AC, Aquino S, Cornelissen JHC (2013) New handbook for standardized measurement of plant functional traits worldwide. Australian Journal of Botany 61, 167–234.
| New handbook for standardized measurement of plant functional traits worldwide.Crossref | GoogleScholarGoogle Scholar |
Reich PB, Ellsworth DS, Walters MB (1998) Leaf structure (specific leaf area) modulates photosynthesis-nitrogen relations: evidence from within and across species and functional groups. Functional Ecology 12, 948–958.
| Leaf structure (specific leaf area) modulates photosynthesis-nitrogen relations: evidence from within and across species and functional groups.Crossref | GoogleScholarGoogle Scholar |
Roff DA (2011) Alternative strategies: the evolution of switch points. Current Biology 21, R285–R287.
| Alternative strategies: the evolution of switch points.Crossref | GoogleScholarGoogle Scholar | 21514512PubMed |
Ryser P, Bernardi J, Merla A (2008) Determination of leaf fresh mass after storage between moist paper towels: constraints and reliability of the method. Journal of Experimental Botany 59, 2461–2467.
| Determination of leaf fresh mass after storage between moist paper towels: constraints and reliability of the method.Crossref | GoogleScholarGoogle Scholar | 18469322PubMed |
Schneider CA, Rasband WS, Eliceiri KW (2012) NIH Image to ImageJ: 25 years of image analysis. Nature Methods 9, 671–675.
| NIH Image to ImageJ: 25 years of image analysis.Crossref | GoogleScholarGoogle Scholar | 22930834PubMed |
Shipley B (2000) Plasticity in relative growth rate and its components following a change in irradiance. Plant, Cell & Environment 23, 1207–1216.
| Plasticity in relative growth rate and its components following a change in irradiance.Crossref | GoogleScholarGoogle Scholar |
Taiz L, Zeiger E (2009) ‘Plant Physiology.’ (Sinauer Associates Inc.: Sunderland, MA, USA)
Tanentzap FM, Stempel A, Ryser P (2015) Reliability of leaf relative water content (RWC) measurements after storage: consequences for in situ measurements. Botany 93, 535–541.
| Reliability of leaf relative water content (RWC) measurements after storage: consequences for in situ measurements.Crossref | GoogleScholarGoogle Scholar |
Thakur D, Rathore N, Chawla A (2019) Increase in light interception cost and metabolic mass component of leaves are coupled for efficient resource use in the high altitude vegetation. Oikos 128, 254–263.
| Increase in light interception cost and metabolic mass component of leaves are coupled for efficient resource use in the high altitude vegetation.Crossref | GoogleScholarGoogle Scholar |
Vaieretti MV, Díaz S, Vile D, Garnier E (2007) Two measurement methods of leaf dry matter content produce similar results in a broad range of species. Annals of Botany 99, 955–958.
| Two measurement methods of leaf dry matter content produce similar results in a broad range of species.Crossref | GoogleScholarGoogle Scholar | 17353207PubMed |
Vile D, Garnier É, Shipley B, Laurent G, Navas ML, Roumet C, Lavorel S, Díaz S, Hodgson JG, Lloret F, Midgley GF, Poorter H, Rutherford MC, Wilson PJ, Wright IJ (2005) Specific leaf area and dry matter content estimate thickness in laminar leaves. Annals of Botany 96, 1129–1136.
| Specific leaf area and dry matter content estimate thickness in laminar leaves.Crossref | GoogleScholarGoogle Scholar | 16159941PubMed |
Violle C, Navas ML, Vile D, Kazakou E, Fortunel C, Hummel I, Garnier E (2007) Let the concept of trait be functional! Oikos 116, 882–892.
| Let the concept of trait be functional!Crossref | GoogleScholarGoogle Scholar |
Wilson PJ, Thompson K, Hodgson JG (1999) Specific leaf area and leaf dry matter content as alternative predictors of plant strategies. New Phytologist 143, 155–162.
| Specific leaf area and leaf dry matter content as alternative predictors of plant strategies.Crossref | GoogleScholarGoogle Scholar |
Wright IJ, Groom PK, Lamont BB, Poot P, Prior LD, Reich PB, Schulze E-D, Veneklaas EJ, Westoby M (2004a) Leaf trait relationships in Australian plant species. Functional Plant Biology 31, 551–558.
| Leaf trait relationships in Australian plant species.Crossref | GoogleScholarGoogle Scholar |
Wright IJ, Reich PB, Westoby M, Ackerly DD, Baruch Z, Bongers F, Cavender-Bares J, Chapin T, Cornellssen JHC, Diemer M, Flexas J, Garnier E, Groom PK, Gulias J, Hikosaka K, Lamont BB, Lee T, Lee W, Lusk C, Midgley JJ, Navas ML, Niinemets Ü, Oleksyn J, Osada H, Poorter H, Pool P, Prior L, Pyankov VI, Roumet C, Thomas SC, Tjoelker MG, Veneklaas EJ, Villar R (2004b) The worldwide leaf economics spectrum. Nature 428, 821–827.
| The worldwide leaf economics spectrum.Crossref | GoogleScholarGoogle Scholar | 15103368PubMed |
Yang D, Niklas KJ, Xiang S, Sun S (2010) Size-dependent leaf area ratio in plant twigs: implication for leaf size optimization. Annals of Botany 105, 71–77.
| Size-dependent leaf area ratio in plant twigs: implication for leaf size optimization.Crossref | GoogleScholarGoogle Scholar | 19864268PubMed |
