Phylogenetic relationships among species of Ganoderma (Ganodermataceae, Basidiomycota) from Cameroon
T. R. Kinge A D , A. M. Mih B and M. P. A. Coetzee C
+ Author Affiliations
- Author Affiliations
A Department of Biological Sciences, Faculty of Science, University of Bamenda, PO Box 39, North West Region, Cameroon.
B Department of Botany and Plant Physiology, Faculty of Science, University of Buea, PO Box 63, South West Region, Cameroon.
C Department of Genetics, Forestry and Agricultural Biotechnology Institute, University of Pretoria, Private Bag X20, Hatfield 0028, South Africa.
D Corresponding author. Email: rosemary32us@yahoo.com
Australian Journal of Botany 60(6) 526-538 https://doi.org/10.1071/BT12011
Submitted: 16 January 2012 Accepted: 21 June 2012 Published: 31 August 2012
Abstract
Ganoderma is an important genus of the Polyporales in the tropics. Identification of tropical species has mainly been based on morphology, which has led to misidentification. This study aimed to elucidate the diversity and phylogenetic relationships of Ganoderma isolates from different hosts in Cameroon using morphological and molecular techniques. Analyses of basidiocarp morphology and the internal transcribed spacer and mitochondria small subunit were undertaken for 28 isolates from five plant species. The results show that the isolates belong to eight species. Three of the species were identified to species level; of these only G. ryvardense has been previously described from Cameroon while G. cupreum and G. weberianum are new records. The five remaining species did not match with any previously described species and have been designated as Ganoderma with different species affinities.
References
Adaskaveg JE, Gilbertson R (1986) Cultural studies and genetics of sexuality of Ganoderma lucidum and G. tsugae in relation to the taxonomy of the G. lucidum complex. Mycologia 78, 694–705.| Cultural studies and genetics of sexuality of Ganoderma lucidum and G. tsugae in relation to the taxonomy of the G. lucidum complex.Crossref | GoogleScholarGoogle Scholar |
Coetzee MPA, Wingfield BD, Kirisits T, Chhetri DB, Bloomer P, Wingfield MJ (2005) Identification of Armillaria isolates from Bhutan based on DNA sequence comparisons. Plant Pathology 54, 36–45.
| Identification of Armillaria isolates from Bhutan based on DNA sequence comparisons.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXit1yjsLs%3D&md5=fb1d9e3448ceb3b4c3ebfa0f13e4e7a1CAS |
Corner EJH (1983) Ad Polyporaceas I. Amauroderma and Ganoderma. Beheift. Nova Hedwigia 75, 1–183.
Douanla-Meli C, Langer E (2009) Ganoderma carocalcareus sp. nov., with crumbly-friable context parasite to saprobe on Anthocleista nobilis and its phylogenetic relationship in G. resinaceum group. Mycological Progress 8, 145–155.
| Ganoderma carocalcareus sp. nov., with crumbly-friable context parasite to saprobe on Anthocleista nobilis and its phylogenetic relationship in G. resinaceum group.Crossref | GoogleScholarGoogle Scholar |
Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.
| Confidence limits on phylogenies: an approach using the bootstrap.Crossref | GoogleScholarGoogle Scholar |
Guindon S, Gascuel O (2003) A simple, fast and accurate algorithm to estimate large phylogenies by maximum likelihood. Systematic Biology 52, 696–704.
| A simple, fast and accurate algorithm to estimate large phylogenies by maximum likelihood.Crossref | GoogleScholarGoogle Scholar |
Hong SG, Jung HS (2004) Phylogenetic analysis of Ganoderma based on nearly complete mitochondrial small-subunit ribosomal DNA sequences. Mycologia 96, 742–755.
| Phylogenetic analysis of Ganoderma based on nearly complete mitochondrial small-subunit ribosomal DNA sequences.Crossref | GoogleScholarGoogle Scholar |
Hong SG, Jeong W, Jung HS (2002) Amplification of mitochondrial small subunit ribosomal DNA of polypores and its potential for phylogenetic analysis. Mycologia 94, 823–833.
| Amplification of mitochondrial small subunit ribosomal DNA of polypores and its potential for phylogenetic analysis.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38XotFOltrg%3D&md5=ddcd692caa20c5923fabd8205db6e667CAS |
Karsten PA (1881) Enumeratio boletinearum et poly-porearum fennicarum. Systemate novo dispositarum. Review Mycology 3, 16–19.
Katoh K, Kuma K, Toh H, Miyata T (2005) MAFFT version 5: improvement in accuracy of multiple sequence alignment. Nucleic Acids Research 33, 511–518.
| MAFFT version 5: improvement in accuracy of multiple sequence alignment.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXhtV2qsbc%3D&md5=c97b75a1e670dcff859992bc9a713449CAS |
Kinge TK, Mih AM (2011) Ganoderma ryvardense sp. nov. associated with basal stem rot disease of oil palm in Cameroon. Mycosphere 2, 179–188.
Lutzoni F, Kauff F, Cox C, Mclauhglin D, Celio G, Dentinger B, Padamsee M, Hibbett D, James T, Baloach E, Grube M, Reeb V, Hofstetter V, Schcoch C, Arnold AE, Miadlikowska J, Spatafora J, Johnson D, Hambleton S, Crockett M, Shoemaker R, Sung GH, Lucking R, Lumbsch T, Donnell OK, Binder M, Diederich P, Ertz D, Gueidan C, Hansen K, Harris RC, Hosaka K, Lim YW, Matheny B, Nishida H, Pfister D, Rogers J, Rossman A, Schmitt I, Sipman H, Stone J, Sugiyama J, Yahr R, Vilgalys R (2004) Assembling the fungal tree of life: progress, classification, and evolution of subcellular traits. American Journal of Botany 91, 1446–1480.
| Assembling the fungal tree of life: progress, classification, and evolution of subcellular traits.Crossref | GoogleScholarGoogle Scholar |
Moncalvo JM, Ryvarden L (1997) A nomenclatural study of the Ganodermataceae. Synopsis Fungorum 11, Fungiflora, Oslo, Norway 1–114.
Moncalvo JM, Wang HF, Hseu RS (1995a) Gene phylogeny of the Ganoderma lucidum complex based on ribosomal DNA sequences. Comparison with traditional taxonomic characters. Mycological Research 99, 1489–1499.
| Gene phylogeny of the Ganoderma lucidum complex based on ribosomal DNA sequences. Comparison with traditional taxonomic characters.Crossref | GoogleScholarGoogle Scholar |
Moncalvo JM, Wang HH, Hseu RS (1995b) Phylogenetic relationships in Ganoderma inferred from the internal transcribed spacers and 25S ribosomal DNA sequences. Mycologia 87, 223–238.
| Phylogenetic relationships in Ganoderma inferred from the internal transcribed spacers and 25S ribosomal DNA sequences.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2MXms12ltbY%3D&md5=b54ee268a91cfe7d6b1931ebea4bba6dCAS |
Nunez M, Daniels (1999) Fungi from the Dja Biosphere Reserve (Cameroon) II. Polypores. Mycotaxon 13, 235–246.
Paterson RM (2007) Ganoderma disease of oil palm – a white rot perspective necessary for integrated control. Crop Protection (Guildford, Surrey) 26, 1369–1376.
| Ganoderma disease of oil palm – a white rot perspective necessary for integrated control.Crossref | GoogleScholarGoogle Scholar |
Pilotti CA, Sanderson FR, Aitken AB, Armstrong W (2004) Morphological variation and host range of two Ganoderma species from Papua New Guinea. Mycopathologia 158, 251–265.
| Morphological variation and host range of two Ganoderma species from Papua New Guinea.Crossref | GoogleScholarGoogle Scholar |
Posada D (2008) jModelTest: phylogenetic model averaging. Molecular Biology and Evolution 25, 1253–1256.
| jModelTest: phylogenetic model averaging.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXotlKgsb4%3D&md5=50647b07ab16a7d5b4fddb0cb0fb670fCAS |
Raeder UP, Broda P (1985) Rapid preparation of DNA from filamentous fungi. Letters in Applied Microbiology 1, 17–20.
| Rapid preparation of DNA from filamentous fungi.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL2MXlvFaltLk%3D&md5=91bee1bd4bcda45f841a406b560a0bc1CAS |
Ronquist F, Huelsenbeck JP (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics (Oxford, England) 19, 1572–1574.
| MrBayes 3: Bayesian phylogenetic inference under mixed models.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXntlKms7k%3D&md5=fb009f7206574775bd8af27eb60276f6CAS |
Ryvarden L (1995) Can we trust morphology in Ganoderma? In ‘Ganoderma: systematics, phytopathology and pharmacolgy, proceedings of contributed symposium 59A, B. Fifth international mycological congress, Vancouver, August 14–21, 1994’. (Eds PK Buchanan, RS Hsue, JM Moncalvo) pp. 19–24. (National Taiwan University: Taipei)
Ryvarden L (2000) Studies in neotropical polypores 2: a preliminary key to neotropical species of Ganoderma with a laccate pileus. Mycologia 92, 180–191.
| Studies in neotropical polypores 2: a preliminary key to neotropical species of Ganoderma with a laccate pileus.Crossref | GoogleScholarGoogle Scholar |
Ryvarden L, Johansen I (1980) ‘A preliminary polypore flora of East Africa.’ (Fungiflora: Oslo) 636 pp.
Singh G (1991) Ganoderma – the scourge of oil palms in the coastal areas. The Planter 67, 421–444.
Smith BJ, Sivasithamparam K (2000a) Internal transcribed spacer ribosomal DNA sequence of five species of Ganoderma from Australia. Mycological Research 104, 943–951.
| Internal transcribed spacer ribosomal DNA sequence of five species of Ganoderma from Australia.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3cXmvVWitrg%3D&md5=d45f0a8fdcd3a5309bee468b573fdaffCAS |
Smith BJ, Sivasithamparam K (2000b) Isozymes of Ganoderma species from Australia. Mycological Research 104, 952–961.
| Isozymes of Ganoderma species from Australia.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3cXmvVWitrk%3D&md5=3e862f666728eafa21696d5633d3fd21CAS |
Steyaert RL (1972) Species of Ganoderma and related genera mainly of the Bogor and Leiden herbaria. Persoonia 7, 55–118.
Swofford DL (2002) ‘PAUP*. Phylogenetic Analysis Using Parsimony (* and other methods), version 4.’ (Sinauer: Sunderland, MA)
Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA 4; Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Molecular Biology and Evolution 24, 1596–1599.
| MEGA 4; Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXpsVGrsL8%3D&md5=bd47fc55681f7cfb27c8dbaa3b3d2672CAS |
Tengoua FF, Bakoume C (2005) Basal stem rot and vascular wilt, two threats for the oil palm sector in Cameroon. The Planter 81, 97–105.
Turner PD (1981) ‘Oil palm diseases and disorders.’ (Oxford University Press: Kuala Lumpur) 281 pp.
Wang DM, Yao YJ (2005) Intrastrain internal transcribed spacer heterogeneity in Ganoderma species. Canadian Journal of Microbiology 51, 113–121.
| Intrastrain internal transcribed spacer heterogeneity in Ganoderma species.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXksFKitbY%3D&md5=4076afabf1bb1935d9340ee2bb6467adCAS |
White TJ, Bruns T, Lee S, Taylor J (1990) Analysis of phylogenetic relationships by amplification and direct sequencing of ribosomal RNA genes. In ‘PCR protocols: a guide to methods and applications’. (Eds MA Innis, DH Gelfand, JJ Sninsky, TJ White) pp. 315–322. (Academic Press: New York)
