Phosphite does not stimulate a wounding response in Eucalyptus marginata seedlings
Roslyn A. Pilbeam A , Kay Howard A , Bryan L. Shearer B and Giles E. St J. Hardy A C
+ Author Affiliations
- Author Affiliations
A School of Biological Sciences, Murdoch University, Perth, WA 6150, Australia.
B Science Division, Department of Environment and Conservation, Locked Bag 104, Bentley Delivery Centre, WA 6983, Australia.
C Corresponding author. Email: G.Hardy@murdoch.edu.au
Australian Journal of Botany 59(4) 393-398 https://doi.org/10.1071/BT10252
Submitted: 22 September 2010 Accepted: 28 March 2011 Published: 9 June 2011
Abstract
Phosphite is used to protect plants from the soilborne plant pathogen, Phytophthora cinnamomi. While several studies have reported a stimulation of defence mechanisms in response to the infection of plants treated with phosphite, the effect of phosphite on abiotic wound repair is unknown. The aim of this histological study was to detail the effects of phosphite on previously undescribed wound repair in Eucalyptus marginata, an important forest tree of south-western Australia, which responds to phosphite treatment. Clonal lines of young plants of E. marginata without a periderm, considered resistant and susceptible to P. cinnamomi, were sprayed with phosphite and the green stems were wounded with liquid nitrogen, where a small area of the vascular cambium was damaged. Transverse hand sections showed phosphite had no effect and there was no genotypic difference on wound responses in E. marginata. Wound periderm and a ligno-suberised boundary zone formed within 7 days. The generation of new phellogen derivatives occurred and by 14 days the vascular cambium was almost fully restored with wound wood formed by 21 days. In the absence of a pathogen, phosphite did not interfere with the quality and speed of wound repair in the E. marginata clones suggesting that wound repair will not be affected when phosphite is used as a prophylactic treatment.
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