Some Conditions Governing Zoospore Production in Axenic Cultures of Phytophthora cinnamomi Rands

Abstract

A method for the production of zoospores from the plant pathogen Phytophthora cinnamomi is described. The use of siliconized cellulose pads as a hyphal support, which in turn allows shake- grown mycelia to be used, results in the production of zoospores in concentrations of 10⁷-10⁸/litre. It allows one person to produce the equivalent of 1-10 mg of zoospore protein per batch, which is sufficient for a variety of biochemical studies.