Effects of Ni on superoxide dismutase and glutathione reductase activities and thiol groups: a comparative study between Alyssum hyperaccumulator and non-accumulator species
S. Majid Ghaderian A C , Rasoul Ghasemi B , Hosniye Heidari A and Saiede Vazirifar A
+ Author Affiliations
- Author Affiliations
A Department of Biology, University of Isfahan, Isfahan 81746-7344, Iran.
B Department of Biology, Faculty of Science, Payame Noor University, Tehran 19395-4697, Iran.
C Corresponding author. Email: ghaderian@sci.ui.ac.ir
Australian Journal of Botany 63(2) 65-71 https://doi.org/10.1071/BT14282
Submitted: 21 October 2014 Accepted: 16 February 2015 Published: 29 April 2015
Abstract
In this study we investigated the activities of superoxide dismutase (SOD), glutathione reductase (GR) and total thiol groups in two Ni hyperaccumulator species, Alyssum inflatum Nyárády and Alyssum bracteatum Boiss. & Bushe, and a non-accumulator species (Alyssum saxatile (L.) Desv.). Plants were exposed to different concentrations of Ni (0, 100, 300 and 500 μM for hyperaccumulators and 0, 10 and 20 μM Ni for the non-accumulator) in solution culture. The accumulation of Ni, the influence of Ni on dry biomass, and SOD, GR and total thiol activities were measured. The results showed a growth retardation effect by Ni in all plants. The activity of SOD strongly decreased with increase in Ni concentration in A. inflatum whereas the other Ni hyperaccumulator, A. bracteatum, showed less decrease. In contrast, a positive link between SOD activity and Ni concentrations was observed in shoots and roots of A. saxatile. The GR and total thiol activities were increased in the hyperaccumulator plants by increase in Ni concentration, but no changes were observed in A. saxatile. The results indicate that in hyperaccumulators, the accumulation of Ni could negatively affect some vital physiological processes.
Additional keywords: detoxification, enzyme activity, serpentine.
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