Anatomy and essential oils from aerial organs in three species of Aldama (Asteraceae–Heliantheae) that have a difficult delimitation
Aline Bertolosi Bombo A B , Tuane Santos De Oliveira A , Adriana Da Silva Santos De Oliveira C , Vera Lúcia Garcia Rehder C , Mara Angelina Galvão Magenta D and Beatriz Appezzato-Da-Glória A E
+ Author Affiliations
- Author Affiliations
A Departamento de Ciências Biológicas, Escola Superior de Agricultura ‘Luiz de Queiroz’, Universidade de São Paulo, C.P. 09, 13418-900, Piracicaba, SP, Brazil.
B Programa de Pós-graduação em Biologia Vegetal, Instituto de Biologia, UNICAMP, C.P. 6109, 13083-970, Campinas, SP, Brazil.
C Centro de Pesquisas Químicas, Biológicas e Agrícolas, CPQBA, UNICAMP, Paulínia, SP, Brazil.
D Universidade Santa Cecília, Santos, SP, Brazil.
E Corresponding author. Email: bagloria@usp.br
Australian Journal of Botany 60(7) 632-642 https://doi.org/10.1071/BT12160
Submitted: 19 June 2012 Accepted: 3 September 2012 Published: 25 October 2012
Abstract
Recently, molecular analysis caused the South American Viguiera Kunth species to be transferred to Aldama La Llave. However, the circumscription has not been established for certain of the South American species, including Aldama filifolia (Sch.Bip. ex Baker) E.E.Schill. & Panero, A. linearifolia (Chodat) E.E.Schill. & Panero and A. trichophylla (Dusén) Magenta (comb. nov.), which had previously been treated as synonyms because of their high similarity. Therefore, the present study aimed to evaluate the anatomy of the aerial organs, and the yield and chemical composition of the essential oils from these three species, to determine the differences among them and thereby assist in species distinction. The anatomical analysis identified characteristics unique to each species, which are primarily related to the position and occurrence of secretory structures. Histochemical analysis demonstrated that the glandular trichomes and the canals secrete lipophilic substances, which are characterised by the presence of essential oils. The analysis of these essential oils identified monoterpenes as their major constituent and allowed for the recognition of chemical markers for each species. The anatomical and chemical characteristics identified by the present study confirmed that the studied samples belong to three distinct taxa.
Additional keywords: Aldama filifolia, Aldama linearifolia, Aldama trichophylla, glandular trichomes, internode anatomy, leaves, monoterpenes, secretory canals.
References
Adams RP (2007) ‘Identification of essential oil components by gas chromatography/mass spectrometry.’ (Allured Publishing Corporation: Carol Stream, IL)Adamson RS (1934) Anomalous secondary thickening in Compositae. Annals of Botany 48, 505–514.
Adedeji O, Jewoola OA (2008) Importance of leaf epidermal characters in the Asteraceae family. Notulae Botanicae Horti Agrobotanici 36, 7–16.
Agostini F, Santos ACA, Rossato M, Pansera MR, Zattera F, Wasum R, Serani LA (2005) Estudo do óleo essencial de algumas espécies do gênero Baccharis (Asteraceae) do sul do Brasil. Revista Brasileira de Farmacognosia 15, 215–219.
| Estudo do óleo essencial de algumas espécies do gênero Baccharis (Asteraceae) do sul do Brasil.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXhtlansLbL&md5=4e7e288f1bdb27bdf06015c6c70520edCAS |
Alvarenga SAV, Ferreira MJP, Emerenciano VP, Cabrol-Bass D (2001) Chemosystematic studies of natural compounds isolated from Asteraceae: characterization of tribes by principal component analysis. Chemometrics and Intelligent Laboratory Systems 56, 27–37.
| Chemosystematic studies of natural compounds isolated from Asteraceae: characterization of tribes by principal component analysis.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXitF2mu7s%3D&md5=fc0fe608f172cf542b281bea21258125CAS |
Alvarenga SAV, Ferreira MJP, Rodrigues GV, Emerenciano VP (2005) A general survey and some taxonomic implications of diterpenes in the Asteraceae. Botanical Journal of the Linnean Society 147, 291–308.
| A general survey and some taxonomic implications of diterpenes in the Asteraceae.Crossref | GoogleScholarGoogle Scholar |
Alvarez L, Mata R, Delgado G, Romo De Vivar A (1985) Sesquiterpene lactones from Viguiera hypargyrea. Phytochemistry 24, 2973–2976.
| Sesquiterpene lactones from Viguiera hypargyrea.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL28XhtVKiu7g%3D&md5=d15a0b0c184c9905d70cc00fc4e2e702CAS |
Ambrosio SR, Schoor K, Da Costa FB (2004) Terpenoids of Viguiera arenaria (Asteraceae). Biochemical Systematics and Ecology 32, 221–224.
| Terpenoids of Viguiera arenaria (Asteraceae).Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXpvVWgt7g%3D&md5=8ceadd9a9f601c21055e7381a1a46b5cCAS |
Appezzato-da-Glória B, Cury G (2011) Morpho-anatomical features of underground systems in six Asteraceae species from the Brazilian Cerrado. Anais da Academia Brasileira de Ciencias 83, 981–992.
| Morpho-anatomical features of underground systems in six Asteraceae species from the Brazilian Cerrado.Crossref | GoogleScholarGoogle Scholar |
Appezzato-da-Glória B, Hayashi AH, Cury G, Soares MKM, Rocha R (2008) Occurrence of secretory structures in underground systems of seven Asteraceae species. Botanical Journal of the Linnean Society 157, 789–796.
| Occurrence of secretory structures in underground systems of seven Asteraceae species.Crossref | GoogleScholarGoogle Scholar |
Baker JG (1884) Compositae IV: Helianthoideae. In ‘Flora Brasiliensis Monachii’. (Eds CFP Martius, AW Eichler) Typografia Regia 6, 135–268.
Blake SF (1918) A revision of the genus Viguiera. Contributions from the Gray Herbarium of Harvard University 51, 1–205.
Borsato AV, Doni-Filho L, Côcco LC, Paglia EC (2007) Rendimento e composição química do óleo essencial da camomila [Chamomilla recutita (L.) Rauschert] submetida à secagem à 70°C. Ciências Agrárias 28, 635–644.
Budel JM, Duarte MR (2007) Caracteres morfoanatômicos de partes vegetativas aéreas de Baccharis coridifolia DC. (Asteraceae–Astereae). Acta Farmaceutica Bonaerense 26, 723–731.
Bukatsch F (1972) Bermerkungen zur Doppelfarbung Astrablau–Safranin. Mikrokosmos 61, 255
Canales M, Hernández T, Rodríguez-Monroy MA, Jiménez-Estrada M, Flores CM, Hernández LB, Gijón IC, Quiroz S, García AM, Ávila G (2008) Antimicrobial activity of the extracts and essential oil of Viguiera dentata. Pharmaceutical Biology 46, 719–723.
| Antimicrobial activity of the extracts and essential oil of Viguiera dentata.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXhsFWmsLrF&md5=0ca56397fbf3b914f11bd9bbaaf0da60CAS |
Castro MM, Leitão-Filho HF, Monteiro WR (1997) Utilização de estruturas secretoras na identificação dos gêneros de Asteraceae de uma vegetação de Cerrado. Revista Brasileira de Botânica 20, 163–174.
| Utilização de estruturas secretoras na identificação dos gêneros de Asteraceae de uma vegetação de Cerrado.Crossref | GoogleScholarGoogle Scholar |
Castro DP, Cardoso MG, Moraes JC, Santos NM, Baliza DP (2006) Não-preferência de Spodoptera frugiperda (Lepidoptera: Noctuidae) por óleos essenciais de Achillea millefolium L. e Thymus vulgaris L. Revista Brasileira de Plantas Medicinais 8, 27–32.
Catalán CAN, Borkosky SA, Joseph-Nathan P (1996) The secondary metabolite chemistry of the subtribe Gochnatiinae (Tribe Mutisieae, Family Compositae). Biochemical Systematics and Ecology 24, 659–718.
Ceccarini L, Macchia M, Flamini G, Cioni PL, Caponi C, Morelli I (2004) Essential oil composition of Helianthus annuus L. leaves and heads of two cultivated hybrids ‘Carlos’ and ‘Florom 350’. Industrial Crops and Products 19, 13–17.
| Essential oil composition of Helianthus annuus L. leaves and heads of two cultivated hybrids ‘Carlos’ and ‘Florom 350’.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXps1Grtbk%3D&md5=44a83843b1505d372fa7c15c6d2c43faCAS |
Corrêa RM, Bertolucci SKV, Pinto JEBP, Reis ES, Alves TL (2004) Rendimento de óleo essencial e caracterização organoléptica de folhas de assa-peixe submetidas a diferentes métodos de secagem. Ciência e Agrotecnologia 28, 339–344.
| Rendimento de óleo essencial e caracterização organoléptica de folhas de assa-peixe submetidas a diferentes métodos de secagem.Crossref | GoogleScholarGoogle Scholar |
Costantin MB, Sartorelli P, Limberger R, Henriques AT, Steppe M, Ferreira MJP, Ohara MT, Emerenciano VP, Kato MJ (2001) Essential oils from Piper cernuum and Piper regnellii: antimicrobial activities and analysis by CG/MS and C-NMR. Planta Medica 67, 771–773.
| Essential oils from Piper cernuum and Piper regnellii: antimicrobial activities and analysis by CG/MS and C-NMR.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXosVylt7g%3D&md5=9faad7863bc53d31f7ef6b0de369a3d5CAS |
Cury G, Appezzato-da-Glória B (2009) Internal secretory spaces in thickened underground systems of Asteraceae species. Australian Journal of Botany 57, 229–239.
| Internal secretory spaces in thickened underground systems of Asteraceae species.Crossref | GoogleScholarGoogle Scholar |
Cysne JB, Canuto KM, Pessoa ODL, Nunes EP, Silveira ER (2005) Leaf essential oils of four Piper species from the State of Ceará – northeast of Brazil. Journal of the Brazilian Chemical Society 16, 1378–1381.
| Leaf essential oils of four Piper species from the State of Ceará – northeast of Brazil.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XktlKnsg%3D%3D&md5=46b6c01c6e6b8b44898846f23345b26eCAS |
Da Costa FB, Vichnewski W, Herz W (1996) Constituents of Viguiera aspillioides and V. robusta. Biochemical Systematics and Ecology 24, 585–587.
| Constituents of Viguiera aspillioides and V. robusta.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK28XntlKmsL8%3D&md5=e66f7e00e641e73a804a3a97925b2834CAS |
Da Costa FB, Shorr K, Arakawa NS, Shilling EE, Spring O (2001) Infraspecific variation in the chemistry of glandular trichomes of two Brazilian Viguiera species (Heliantheae; Asteraceae). Journal of the Brazilian Chemical Society 12, 403–407.
| Infraspecific variation in the chemistry of glandular trichomes of two Brazilian Viguiera species (Heliantheae; Asteraceae).Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXktFejtLc%3D&md5=226e9ae703e6541d97d62a21e105f347CAS |
David R, Carde JP (1964) Coloration différentielle des inclusions lipidique et terpeniques des pseudophylles du Pin maritime au moyen du reactif Nadi. Comptes Rendus de l’Académie des Sciences 258, 1338–1340.
Del-Vechio-Vieira G, Barbosa MVD, Lopes BC, Sousa OV, Santiago-Fernandes LDR, Esteves RL, Kaplan MAC (2008) Caracterização morfoanatômica de Ageratum fastigiatum (Asteraceae). Revista Brasileira de Farmacognosia 18, 769–776.
| Caracterização morfoanatômica de Ageratum fastigiatum (Asteraceae).Crossref | GoogleScholarGoogle Scholar |
Delgado G, Alvarez L, Romo De Vivar A (1984) Terpenoids and a flavan-3-ol from Viguiera quinqueradiata. Phytochemistry 23, 675–678.
| Terpenoids and a flavan-3-ol from Viguiera quinqueradiata.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL2cXktlGrtbk%3D&md5=b53cc396209eaf96e0e32fbb48310cd6CAS |
Dickison WC (2000) ‘Integrative plant anatomy.’ (Academic Press: San Diego, CA)
Emerenciano VP, Ferreira ZS, Kaplan MAC, Gottlieb OR (1987) A chemosystematic analysis of tribes of Asteraceae involving sesquiterpene lactones and flavonoids. Phytochemistry 26, 3103–3115.
| A chemosystematic analysis of tribes of Asteraceae involving sesquiterpene lactones and flavonoids.Crossref | GoogleScholarGoogle Scholar |
Emerenciano VP, Milit JSLT, Campos CC, Romoff P, Kaplan MAC, Zambon M, Brant AJC (2001) Flavonoids as chemotaxonomic markers for Asteraceae. Biochemical Systematics and Ecology 29, 947–957.
| Flavonoids as chemotaxonomic markers for Asteraceae.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXltVaksLg%3D&md5=183ac1ed103ab98b60600bc5cb8f6017CAS |
Empinotti CB, Duarte MR (2008) Estudo anatômico de folha e caule de Elephantopus mollis Kunth (Asteraceae). Revista Brasileira de Farmacognosia 18, 108–116.
| Estudo anatômico de folha e caule de Elephantopus mollis Kunth (Asteraceae).Crossref | GoogleScholarGoogle Scholar |
Fahn A (1979) ‘Secretory tissues in plants.’ (Academic Press: London)
Fahn A (2000) Structure and function of secretory cells. Advances in Botanical Research 31, 37–75.
| Structure and function of secretory cells.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3cXltFKgsLw%3D&md5=a47d1be5ba0f5f25af4bf0325a115726CAS |
Ferronatto R, Marchesan ED, Pezenti E, Bednarski F, Onofre SB (2007) Atividade antimicrobiana de óleos essenciais produzidos por Baccharis dracunculifolia D.C. e Baccharis uncinella D.C. (Asteraceae). Revista Brasileira de Farmacognosia 17, 224–230.
| Atividade antimicrobiana de óleos essenciais produzidos por Baccharis dracunculifolia D.C. e Baccharis uncinella D.C. (Asteraceae).Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXot1KmtLk%3D&md5=e02b2bea571f566c24a864627490567fCAS |
Flamini G, Ertugrul K, Cioni PL, Morelli I, Dural H, Bagci Y (2002) Volatile constituents of two endemic Centaurea species from Turkey: C. pseudoscabiosa subsp. pseudoscabiosa and C. hadimensis. Biochemical Systematics and Ecology 30, 953–959.
| Volatile constituents of two endemic Centaurea species from Turkey: C. pseudoscabiosa subsp. pseudoscabiosa and C. hadimensis.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38XnvVGqt7k%3D&md5=afcb4e266dc3b1df1fbd8f56f2d8b325CAS |
Fritz E, Saukel J (2011) Secretory structures of subterranean organs of some species of the Cardueae, and their diagnostic value. Acta Biologica Cracoviensia. Series; Botanica 53, 63–73.
| Secretory structures of subterranean organs of some species of the Cardueae, and their diagnostic value.Crossref | GoogleScholarGoogle Scholar |
Gregio SJD, Moschetta IS (2006) Anatomia de raiz, caule e folha e identificação de estruturas secretoras de Achillea millefolium L. (Asteraceae). Acta Scientiarum Biological Sciences 28, 327–334.
Harborne JB, Turner BL (1984) ‘Plant chemosystematics.’ (Academic Press: London)
Horridge GA, Tamm SL (1969) Critical point drying for scanning electron microscopy study of ciliary motion. Science 163, 817–818.
| Critical point drying for scanning electron microscopy study of ciliary motion.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BC3cvjsl2ktA%3D%3D&md5=4c6d73a36dfa6553ba4f3e8c8d110e7aCAS |
Hulley IM, Viljoen AM, Tilney PM, Van Vuuren SF, Kamatou GPP, Van Wyk BE (2010) The ethnobotany, leaf anatomy, essential oil variation and biological activity of Pteronia incana (Asteraceae). South African Journal of Botany 76, 668–675.
| The ethnobotany, leaf anatomy, essential oil variation and biological activity of Pteronia incana (Asteraceae).Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXhtlKjs7fF&md5=27140cf2d430c0cf4f59c93fa3f8376aCAS |
Jensen WA (1962) ‘Botanical histochemistry: principle and practice.’ (W. H. Freeman: San Francisco)
Johansen DA (1940) ‘Plant microtechnique.’ (McGraw-Hill: New York)
Karnovsky MJ (1965) A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy. The Journal of Cell Biology 27, 137–138.
Kelsey RG (1984) Glandular trichomes: a helpful taxonomic character of Artemisia nova (black sagebrush). Journal of Range Management 37, 370–372.
| Glandular trichomes: a helpful taxonomic character of Artemisia nova (black sagebrush).Crossref | GoogleScholarGoogle Scholar |
King BL, Jones SB (1982) Chemosystematics of Vernonia series Flexuosae (Vernonieae; Compositae). Bulletin of the Torrey Botanical Club 109, 279–286.
| Chemosystematics of Vernonia series Flexuosae (Vernonieae; Compositae).Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL3sXislaq&md5=82f7d31abc8722d1499baa91f936a39cCAS |
Leite AM, Lima EO, Souza EL, Diniz MFFM, Trajano VN, Medeiros IA (2007) Inhibitory effect of β-pinene, α-pinene and eugenol on the growth of potential infectious endocarditis causing Gram-positive bacteria. Revista Brasileira de Ciências Farmacêuticas 43, 121–126.
| Inhibitory effect of β-pinene, α-pinene and eugenol on the growth of potential infectious endocarditis causing Gram-positive bacteria.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXnt1ahsr8%3D&md5=2f681a481319151689dda878bd51a050CAS |
Lersten NR, Curtis JD (1988) Secretory reservoirs (ducts) of two kinds in giant ragweed (Ambrosia trifida; Asteraceae). American Journal of Botany 75, 1313–1323.
| Secretory reservoirs (ducts) of two kinds in giant ragweed (Ambrosia trifida; Asteraceae).Crossref | GoogleScholarGoogle Scholar |
Lersten NR, Curtis JD (1989) Foliar oil reservoir anatomy and distribution in Solidago canadensis (Asteraceae, tribe Astereae). Nordic Journal of Botany 9, 281–287.
| Foliar oil reservoir anatomy and distribution in Solidago canadensis (Asteraceae, tribe Astereae).Crossref | GoogleScholarGoogle Scholar |
Lorenzetti BB, Souza GE, Sarti SJ, Santos Filho D, Ferreira SH (1991) Myrcene mimics the peripheral analgesic activity of lemongrass tea. Journal of Ethnopharmacology 34, 43–48.
| Myrcene mimics the peripheral analgesic activity of lemongrass tea.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK38XlslA%3D&md5=abe60bd33f0066837267f6927dcc8fafCAS |
Lotocka B, Geszprych A (2004) Anatomy of the vegetative organs and secretory structures of Rhaponticum carthamoides (Asteraceae). Botanical Journal of the Linnean Society 144, 207–233.
| Anatomy of the vegetative organs and secretory structures of Rhaponticum carthamoides (Asteraceae).Crossref | GoogleScholarGoogle Scholar |
Lotocka B, Osinska E (2010) Shoot anatomy and secretory structures in Hypericum species (Asteraceae). Botanical Journal of the Linnean Society 163, 70–86.
| Shoot anatomy and secretory structures in Hypericum species (Asteraceae).Crossref | GoogleScholarGoogle Scholar |
Magenta MAG (2006) Viguiera Kunth (Asteraceae, Heliantheae) na América do Sul e sistemática das espécies do Brasil. PhD Thesis, University of São Paulo, Brazil.
Magenta MAG, Pirani JR, Mondin CA (2010) Novos táxons e combinações em Viguiera (Asteraceae–Heliantheae). Rodriguesia 61, 1–11.
Maia AIV, Torres MCM, Pessoa ODL, Menezes JESA, Costa SMO, Nogueira VLR, Melo VMM, Souza EB, Cavalcante MGB, Albuquerque MRJR (2010) Óleos essenciais das folhas de Vernonia remotiflora e Vernonia brasiliana: composição química e atividade biológica. Quimica Nova 33, 584–586.
| Óleos essenciais das folhas de Vernonia remotiflora e Vernonia brasiliana: composição química e atividade biológica.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXlt1yhur4%3D&md5=74e00a8ff6d1b5c6f66516f582fdd6e8CAS |
Marquina S, Maldonado N, Garduño-Ramírez ML, Aranda E, Villarreal ML, Navarro V, Bye R, Delgado G, Alvarez L (2001) Bioactive oleanolic acid saponins and other constituents from the roots of Viguiera decurrens. Phytochemistry 56, 93–97.
| Bioactive oleanolic acid saponins and other constituents from the roots of Viguiera decurrens.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXitlaruw%3D%3D&md5=5e44322b37a595f481ffa3ab21bf7178CAS |
Metcalfe CR, Chalk L (1950) ‘Anatomy of the dicotyledons.’ (Clarendon Press: Oxford, UK)
Metcalfe CR, Chalk L (1983) ‘Anatomy of the dicotyledons: wood struture and conclusion of the general introduction.’ (Oxford University Press: New York)
Milan P, Hayashi AH, Appezzato-Da-Glória B (2006) Comparative leaf morphology and anatomy of three Asteraceae species. Brazilian Archives of Biology and Technology 49, 135–144.
| Comparative leaf morphology and anatomy of three Asteraceae species.Crossref | GoogleScholarGoogle Scholar |
Moronkola DO, Ogunwande IA, Walker TM, Setzer WN, Oyewole IO (2007) Identification of the main volatile compounds in the leaf and flower of Tithonia diversifolia (Hemsl) Gray. Journal of Natural Medicines 61, 63–66.
| Identification of the main volatile compounds in the leaf and flower of Tithonia diversifolia (Hemsl) Gray.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXht1ShtLc%3D&md5=3ef95f3a7f3cceda6a1166c9402ec26cCAS |
Pearse AGE (1968) ‘Histochemistry. Theoretical and applied.’ (J. e A. Churchill Ltd: London)
Robinson H, Moore AJ (2004) New species and new combinations in Rhysolepis (Heliantheae – Asteracae). Proceedings of the Biological Society of Washington 117, 423–446.
Sakai WS (1973) Simple Method for differential staining of paraffin embedded plant material using toluidine blue. Stain Technology 48, 247–248.
Schilling EE, Panero JL (2011) A revised classification of subtribe Helianthinae (Asteraceae: Heliantheae) II. Derived lineages. Botanical Journal of the Linnean Society 167, 311–331.
| A revised classification of subtribe Helianthinae (Asteraceae: Heliantheae) II. Derived lineages.Crossref | GoogleScholarGoogle Scholar |
Silva L, Oniki GH, Agripino DG, Moreno PRH, Young MCM, Mayworm MAS, Ladeira AM (2007) Biciclogermacreno, resveratrol e atividade antifúngica em extratos de folhas de Cissus verticillata (L.) Nicolson e Jarvis (Vitaceae). Revista Brasileira de Farmacognosia 17, 361–367.
| Biciclogermacreno, resveratrol e atividade antifúngica em extratos de folhas de Cissus verticillata (L.) Nicolson e Jarvis (Vitaceae).Crossref | GoogleScholarGoogle Scholar |
Solereder H (1908) ‘Systematic anatomy of the dicotyledons: a handbook for laboratories of pure and applied botany.’ (Clarendon Press: Oxford, UK)
Sousa OV, Dutra RC, Yamamoto CH, Pimenta DS (2008) Estudo comparativo da composição química e da atividade biológica dos óleos essenciais das folhas de Eremanthus erythropappus (DC) McLeisch. Revista Brasileira de Farmácia 89, 113–116.
Souza TJT, Apel MA, Bordignon S, Matzenbacher NI, Zuanazzi JAS, Henriques AT (2007) Composição química e atividade antioxidante do óleo volátil de Eupatorium polystachyum DC. Revista Brasileira de Farmacognosia 17, 368–372.
| Composição química e atividade antioxidante do óleo volátil de Eupatorium polystachyum DC.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXht1Gks7zI&md5=5fbab5bf91bc52ffb82ef7b5f55c31b1CAS |
Spring O, Zipper R, Reeb S, Vogler B, Da Costa FB (2001) Sesquiterpenes lactones and a myoinositol from glandular trichomes of Viguiera quinqueremis (Heliantheae; Asteraceae). Phytochemistry 57, 267–272.
| Sesquiterpenes lactones and a myoinositol from glandular trichomes of Viguiera quinqueremis (Heliantheae; Asteraceae).Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXjtFCnu78%3D&md5=b6711726f485e404728aace6f6ed7d36CAS |
Spring O, Zipper R, Conrad J, Vogler B, Klaiber I, Da Costa FB (2003) Sesquiterpene lactones from glandular trichomes of Viguiera radula (Heliantheae; Asteraceae). Phytochemistry 62, 1185–1189.
| Sesquiterpene lactones from glandular trichomes of Viguiera radula (Heliantheae; Asteraceae).Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXitFaitLg%3D&md5=14a0c35670a43a0e47470afd92a4d4c2CAS |
Strasburger E (1913) ‘Handbook of practical botany.’ (George Allen e Company Ltda: London)
Tirapelli CR, Ambrosio SR, Da Costa FB, Oliveira AM (2004) Evidence for the mechanisms underlying the effects of pimaradienoic acid isolated from the roots of Viguiera arenaria on rat aorta. Pharmacology 70, 31–38.
| Evidence for the mechanisms underlying the effects of pimaradienoic acid isolated from the roots of Viguiera arenaria on rat aorta.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXpsVWht74%3D&md5=f9923fa720eb6f87dd79f641f12b9095CAS |
Wagenitz G (1976) Systematics and phylogeny of the Compositae (Asteraceae). Plant Systematics and Evolution 125, 29–46.
| Systematics and phylogeny of the Compositae (Asteraceae).Crossref | GoogleScholarGoogle Scholar |
