Understanding male sterility in Miconia species (Melastomataceae): a morphological approach
Priscila Andressa Cortez A , Sandra Maria Carmello-Guerreiro A and Simone Pádua Teixeira B C
+ Author Affiliations
- Author Affiliations
A Departamento de Biologia Vegetal, Instituto de Biologia, Universidade Estadual de Campinas, Caixa Postal 6109, Campinas, SP, 13083-970, Brazil.
B Departamento de Ciências Farmacêuticas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Avenida do Café, s/n, Ribeirão Preto, SP, 14040-903, Brazil.
C Corresponding author. Email: spadua@fcfrp.usp.br
Australian Journal of Botany 60(6) 506-516 https://doi.org/10.1071/BT12076
Submitted: 31 March 2012 Accepted: 14 June 2012 Published: 12 September 2012
Abstract
Pollen abortion occurs in virtually all species and often does not prejudice reproductive success. However, large numbers of abnormal pollen grains are characteristic of some groups. Among them is Miconia, in which partial and complete male sterility is often related to apomixis. In this study, we compared the morphology of pollen grains over several developmental stages in Miconia species with different rates of male sterility. Our aim was to improve the knowledge of mechanisms that lead to male sterility in this ecologically important tropical group. Routine techniques for microscopy were used to examine anthers in several developmental stages collected from the apomictic species Miconia albicans and M. stenostachya. Both species are completely male sterile since even the pollen grains with apparently normal cytoplasm were not able to develop a pollen tube. Meiosis is a rare event in M. albicans anthers and happens in an irregular way in M. stenostachya, leading to the pollen abortion. M. albicans has more severe abnormalities than M. stenostachya since even the microspores and pollen grain walls were affected. Moreover, in M. stenostachya, most mitosis occurring during microgametogenesis was also abnormal, leading to the formation of bicellular pollen grains with two similar cells, in addition to the formation of pollen grains of different sizes. Notably, abnormalities in both species did not reach the production of Übisch bodies, suggesting little or no tapetum involvement in male sterility in these two species.
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