Comparative study on the effects of NaCl on selected moss and fern representatives
Milica Bogdanović A D , Milena Ilić A , Suzana Živković A , Aneta Sabovljević B , Dragoljub Grubišić A B C and Marko Sabovljević B
+ Author Affiliations
- Author Affiliations
A Institute for Biological Research Siniša Stanković, University of Belgrade, Bulevar Despota Stefana 142, 11000 Belgrade, Serbia.
B Institute of Botany and Botanical Garden, Faculty of Biology, University of Belgrade, Takovska 43, 11000 Belgrade, Serbia.
C Deceased.
D Corresponding author. Email: milica84bog@gmail.com
Australian Journal of Botany 59(8) 734-740 https://doi.org/10.1071/BT11059
Submitted: 18 February 2011 Accepted: 8 November 2011 Published: 23 January 2012
Abstract
Salt demonstrates various osmotic and ionic effects on vascular plant growth, development and function, but very few data can be found on how salt affects non-tracheophytes. To explore this, gametophytes of two moss – Bryum argenteum Hedw. and Atrichum undulatum (Hedw.) P. Beauv., and three fern species – Asplenium viride Britton, Ceterach officinarum DC, and Phyllitis scolopendrium (L.) Newman, were treated for 3 days with different NaCl concentrations in growth medium under in vitro controlled conditions. Subsequently, these plants recovered for 18 days on NaCl-free medium, after which the following parameters were measured for mosses: presence of secondary protonema and shoots, protonemal radius and index of multiplication. Survival, chlorophyll a, b, total and a/b ratio were determined as well as total phenolic content, both for ferns and mosses. All species tolerated 50 and 100 mM of NaCl-enriched media, quite well. On higher salt concentrations in the substrata, measured morphological parameters and chlorophyll content were reduced. In general, mosses exhibited higher NaCl tolerance than ferns. Change of phenolic content in ferns suggests these plants use antioxidative properties of phenolics as a mechanism of salt tolerance, in contrast with mosses whose phenolic content was stable.
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