Mycosporine-like amino acids profile and their activity under PAR and UVR in a hot-spring cyanobacterium Scytonema sp. HKAR-3
Rajesh P. Rastogi A ,A Laboratory of Photobiology and Molecular Microbiology, Centre of Advanced Study in Botany, Banaras Hindu University, Varanasi-221005, India.
B Department of Biology, Friedrich-Alexander University, Erlangen-Nuremberg, Staudtstrasse 5, D-91058 Erlangen, Germany.
C Corresponding author. Email: r.p.sinha@gmx.net
Australian Journal of Botany 58(4) 286-293 https://doi.org/10.1071/BT10004
Submitted: 9 January 2010 Accepted: 30 April 2010 Published: 22 June 2010
Abstract
The cyanobacterium Scytonema sp. HKAR-3 isolated from a hot spring in India was investigated for the presence of mycosporine-like amino acids (MAAs) and their induction under PAR and PAR+UVR. High-performance liquid-chromatograph (HPLC) analysis revealed the presence of two MAAs, mycosporine–glycine (λmax = 310 nm) and an unknown MAA-334 (λmax = 334 nm), with retention times of 4.1 and 8.7 min, respectively. This is the first report for the presence of two MAAs and, in particular, the synthesis of mycosporine–glycine in any strain of Scytonema. There was no effect of radiation type (PAR or PAR+UVR) on the synthesis of mycosporine–glycine; however, the synthesis of MAA-334 was found to be higher in samples receiving PAR+UVR than in those receiving PAR only. There was a circadian induction in the synthesis of MAAs under alternate 12-h light (PAR or PAR+UVR) and dark periods. MAAs were found to be induced mostly during the light period and their production dropped again during the dark period. This suggests that the synthesis of MAAs is an energy-dependent process and depends on solar energy for its maintenance in natural habitats.
Acknowledgements
R. P. Rastogi is thankful to University Grants Commission, New Delhi, India, for financial support in the form of a fellowship.
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