Investigating DNA barcoding options for the identification of Caladenia (Orchidaceae) species
Lachlan Farrington A B , Phyllis MacGillivray A B , Renate Faast A and Andrew Austin A B CA School of Earth and Environmental Sciences, The University of Adelaide, SA 5005, Australia.
B Australian Centre for Evolutionary Biology and Biodiversity, The University of Adelaide, SA 5005, Australia.
C Corresponding author. Email: andy.austin@adelaide.edu.au
Australian Journal of Botany 57(4) 276-286 https://doi.org/10.1071/BT08138
Submitted: 30 June 2008 Accepted: 17 March 2009 Published: 29 July 2009
Abstract
The application of molecular techniques for defining evolutionary units in Caladenia has largely focussed on addressing relationships at the subgeneric and deeper levels. However, in light of the morphological complexity present in this diverse genus, molecular markers offer additional characters for the refinement of taxonomy at the species level. In the present study, we explored the utility of marker systems with demonstrated application for defining fine-scale units, both in terms of phylogenetic information and in the context of DNA barcoding. We also provide a working example of the use of molecular techniques for identifying the source plants of pollinia collected from passively sampled pollinators and for identifying sterile plants.
Acknowledgements
The authors acknowledge and value the contribution of working professionals and amateurs for the collection of samples and provision of orchid-population locations. Specifically, we thank Chris Obst (Environmental and Biodiversity Services), Andrew Pritchard (DSE Victoria), Joe Quarmby and Cath Dickson (DEHSA), David Kilpin, Jenny Skinner, Andy Young and members of the Native Orchid Society of South Australia, the Friends of Onkaparinga National Park and the Friends of Spring Gully Conservation Park. We thank Stephen Donnellan, Kathy Saint and Leanne Wheaton from the Evolutionary Biology Unit, South Australian Museum for technical assistance and advice. Dr Nick Murphy provided guidance with phylogenetic analysis and valuable comments on the manuscript, as did other members of the Austin laboratory group. All samples were collected under Department for Environment and Heritage permit No. C25273. This research was funded through an ARC linkage project with industry partners DEHSA, the South Australian Museum, Earth Sanctuaries Foundation of Australia Inc. and the Royal Zoological Society of South Australia Inc., in addition to support under the Native Vegetation Council (SA) grants-funding scheme.
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