Ontogenesis of the fruit pulp layer of Hymenaea stigonocarpa (Fabaceae: Caesalpinioideae)
E. A. S. Paiva A C and D. M. T. Oliveira BA Departamento de Botânica, ICB, UFMG, Belo Horizonte, MG, CEP 31.270-901, Brazil.
B Departamento de Botânica, IB, UNESP, CP510, Botucatu, SP, CEP 18.618-000, Brazil.
C Corresponding author. Email: epaiva@icb.ufmg.br
Australian Journal of Botany 52(5) 677-683 https://doi.org/10.1071/BT03054
Submitted: 14 May 2003 Accepted: 14 May 2004 Published: 25 October 2004
Abstract
Hymenaea, a genus of major economic importance, has been the subject of several botanical studies. However, there is disagreement over the origin of the edible fruit pulp of Hymenaea, as there are no ontogenetic studies on this organ. According to some authors, the edible layer results from transformations of the mesocarp and endocarp, while according to others, it is considered a seed aril. There are still others who regard this layer as originating from an undefined region of the pericarp. To understand the nature and origin of the pulp layer, Hymenaea stigonocarpa Mart. ex Hayne ovaries and fruit were processed according to standard techniques. The production of the fruit pulp layer starts immediately after anthesis. During anthesis, the inner epidermal cells of the ovary show periclinal division and form a new layer of cells towards the mesocarp; this remains meristematic and initiates cell production by predominantly periclinal divisions, producing a compact tissue towards the locule. This tissue will become the fruit pulp layer, the inner endocarp. The seed coat shows typical testal structure without evidence of aril formation. This allows us to conclude that the fruit pulp layers are exclusively made from part of the endocarp. We also observed resin cavities on outer mesocarp and outer endocarp.
Acknowledgments
We thank FAPESP for financial support (2001/00345-0 and 2000/12649-3).
Almeida, SP ,
Proença, CEB ,
Sano, SM ,
and
Ribeiro, JF (1998).
Barroso, GM ,
Morim, MP ,
Peixoto, AL ,
and
Ichaso, CLF (1999).
Burkart, A (1952).
Cowan RS, Polhill RM
(1981) Tribe 4. Detarieae DC. (1825) ‘Advances in legume systematics’. (Eds RM Polhill, PH Raven)
pp. 117–134. (Royal Botanic Gardens: Kew)
Correa, P ,
and
Penna, LA (1969).
Fahn, A (1990).
Jensen, WA (1962).
Johansen, DA (1940).
Karnovsky MJ
(1965) A formaldehyde–glutaraldehyde fixative of hight osmolality for use in electron microscopy. The Journal of Cell Biology 27, 137A–138A.
Lee YT, Langenheim JH
(1975) A systematic revision of the genus Hymenaea (Leguminosae; Caesalpinioideae; Detarieae). University of California Publication in Botany 69, 1–109.
Mauseth, JD (1988).
Martins MAG, Oliveira DMT
(2001) Morfo-anatomia e ontogênese do fruto e semente de Tipuana tipu (Benth.) O.Kuntze (Fabaceae: Faboideae). Revista Brasileira de Botânica 24, 109–121.
Nazif MJ
(1914) Sur le fruit des Hymenaea. Bulletin de la Société Botanique de Genève 11, 29–32.
O’Brien TP,
Feder N, McCully ME
(1964) Polychromatic staining of plant cell walls by toluidine blue O. Protoplasma 59, 368–373.
Oliveira DMT, Beltrati CM
(1993) Aspectos anatômicos dos frutos e sementes em desenvolvimento de Inga fagifolia (Fabaceae: Mimosoideae). Revista Brasileira de Biologia 53, 625–636.
Pate JS, Kuo J
(1981) Anatomical studies of legume pods—a possible tool in taxonomic research. ‘Advances in legume systematics’. (Eds RM Polhill, PH Raven)
pp. 903–912. (Royal Botanic Gardens: Kew)
Romberger, JA ,
Hejnowicz, Z ,
and
Hill, JF (1983).
Roth, I (1977).
Sass, JE (1951).
