Translation of Rabbit Haemoglobin mRNA in Oocytes of the Queensland Cane Toad, Bufo marinus

Abstract

Rabbit haemoglobin mRNA is translated in oocytes of the toad, B. marinus. The product was identified by Sephadex chromatography, tryptic peptide analysis and CM-cellulose chromatography using authentic rabbit haemoglobin as a marker. Oocytes of this species provide a suitable alternative to those of Xenopus laevis for the detection of mRNA.