Subunits From Reduced and S-Carboxymethylated Ribulose Diphosphate Carboxylase (Fraction I Protein)

Abstract

Fraction I protein isolated from spinach beet chloroplasts was purified by ammonium sulphate fractionation and Sephadex G·200 gel filtration. The isolated protein was reduced and S-carboxymethylated, and the dissociated protein resolved into two distinct subunits by gel filtration on Sephadex G-200 in an 8M urea buffer at pH 10·0.