Studies On Marsupial Proteins I. Polymorphism of Haemoglobin of the Grey Kangaroo, Macropus Giganteus

Abstract

A haemolysate of red blood cells of the grey kangaroo, M. giganteu8, gave several haemoglobin peaks when chromatographed on polycarboxylic-acid-type ion-exchange resins. In the four samples studied two major components, haemoglobin I and II, were present. In the sample examined in detail these comprised 51 and 45% respectively, together with about 4% of minor haem-containing proteins. The two major haemoglobin components were readily separated by chromatography at pH 6·4 in phosphate buffer of 0 ·152M sodium-potassium ion concentration.