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RESEARCH ARTICLE
Contents Vol 47(10)

Myofibre characteristics of ovine longissimus and semitendinosus muscles are influenced by sire breed, gender, rearing type, age and carcass weight

P. L. Greenwood A B E , S. Harden A C and D. L. Hopkins A D
+ Author Affiliations
- Author Affiliations

A Australian Sheep Industry Cooperative Research Centre, Armidale, NSW 2350, Australia.

B NSW Department of Primary Industries Beef Industry Centre of Excellence, University of New England, Armidale, NSW 2351, Australia.

C NSW Department of Primary Industries, Tamworth Agricultural Institute, Tamworth, NSW 2340, Australia.

D NSW Department of Primary Industries, Centre for Sheep Meat Development, Cowra, NSW 2794, Australia.

E Corresponding author. Email: paul.greenwood@dpi.nsw.gov.au

Australian Journal of Experimental Agriculture 47(10) 1137-1146 https://doi.org/10.1071/EA06324
Submitted: 8 January 2007  Accepted: 16 May 2007   Published: 19 September 2007

Abstract

The objective of this study was to determine the extent to which sire breed (Merino, primarily a wool-producing breed, and Poll Dorset, primarily a meat-producing breed), gender (castrate and ewe), rearing type (single- or multiple-reared), age (4, 8, 14 and 22 months) and carcass weight influence ovine (n = 204) myofibre characteristics. In Merino-sired sheep, the relative area (% of total myofibre area) of type 2X myofibres was lower and of type 2A myofibres higher in the longissimus lumborum (longissimus) muscle than in Poll Dorset-sired sheep. Female sheep had a higher relative area of type 2X and a lower relative area of type 2A myofibres than castrate sheep in the longissimus muscle. The percentage of type 2A myofibres increased and of type 2X myofibres decreased in the longissimus between 4 and 22 months of age. Type 1 myofibres in the semitendinosus muscle were more prevalent and type 2X myofibres less prevalent in Merino than in Poll Dorset-sired sheep. The percentage of type 1 myofibres was lower in female than in castrate sheep. Age-related effects on semitendinosus myofibre characteristics varied with gender, sire breed and rearing type. Influences of rearing type on myofibre characteristics were evident in the semitendinosus muscle at younger ages, but did not persist to 22 months of age. The greatest increases in myofibre cross-sectional areas occurred between 8 and 14 months of age in the longissimus muscle, and between 14 and 22 months of age in the semitendinosus muscle. Carcass weight significantly influenced cross-sectional area of type 2A, 2AX and 2X myofibres in longissimus and semitendinosus muscles. It is concluded that influences of sire breed, gender, rearing type, age and carcass weight on ovine myofibre percentages varies between longissimus and semitendinosus muscles. Furthermore, the pattern of postnatal myofibre growth differs between these muscles.

Additional keywords: lamb, genotype, myofibre types, myosin heavy chains, skeletal muscle.


Acknowledgements

We wish to thank Amanda Lang, Stuart McClelland, Joe Brunner, Bill Johns and Steve Sinclair (NSW DPI Beef Industry Centre of Excellence, Armidale) for the conduct of laboratory procedures and inputs into abattoir sampling and data collection. The animals sampled for this paper were generated at the NSW Department of Primary Industries, Centre for Sheep Meat Development, Cowra as part of the Australian Sheep Industry Cooperative Research Centre. The team at Cowra is thanked for their management of the flock and the efficient execution of the slaughter program. Specifically, we wish to thank David Stanley (NSW DPI) who has managed the database arising from this large collaborative program and the team members from other research groups who assisted with data collection. The kind provision of antibody S5-8H2 by Dr Brigitte Picard is also gratefully acknowledged, as is the advice of Dr Arthur Gilmour (NSW DPI) on the design and statistical analyses of this experiment.


References


Anon.  (2004) Estimated breeding value (EBV) definitions: Concise definitions for estimated breeding values. Available at http://abri.une.edu.au/online/pages/understanding_lambplan_ebvs.htm.

Arguello A, Lopez-Fernandez JL, Rivero JL (2001) Limb myosin heavy chain isoproteins and muscle fibre types in the adult goat (Capra hircus). The Anatomical Record 264, 284–293.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Arnold AM, Meyer HH (1988) Influences of gender, time of castration, genotype, birthtype and feeding regimen on lamb longissimus fiber type proportions. Journal of Animal Science 66, 2476–2483. open url image1

Banks RG (1994) LAMBPLAN: genetic evaluation for the Australian lamb industry. In ‘Proceedings of the 5th world congress on genetics applied to livestock production. Vol. 18’. (Eds C Smith, JS Gavora, B Benkel, J Chesnais, W Fairfull, JP Gibson, BW Kennedy, EB Burnside) pp. 15–18. (University of Guelph: Guelph, ON)

Briand M, Talmant A, Briand Y, Monin G, Durand R (1981) Metabolic types of muscle in the sheep. I. Myosin ATPase, glycolytic, and mitochondrial enzyme activities. European Journal of Applied Physiology 46, 347–358.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Butterfield RM (1988) ‘New concepts of sheep growth.’ (Griffin Press: Netley, SA)

Carpenter CE, Owen DR, Cockett NE, Snowder GD (1996) Histology and composition of muscles from normal and Callipyge lambs. Journal of Animal Science 74, 388–393. open url image1

Gardner GE, Pethick DW, Greenwood PL, Hegarty RS (2006) The effect of genotype and plane of nutrition on the rate of pH decline in lamb carcasses and the expression of metabolic enzymatic markers. Australian Journal of Agricultural Research 57, 661–670.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Gardner GE, Hopkins DL, Greenwood PL, Cake MA, Boyce MD, Pethick DW (2007) Sheep genotype, age and muscle type affect the expression of metabolic enzyme markers. Australian Journal of Experimental Agriculture 47, 1180–1189. open url image1

Gilmour AR, Cullis BR, Gogel GJ, Welham SJ, Thompson R (2002) ‘ASRemL user guide, Release 1.0.’ (NSW Agriculture: Orange, NSW)

Greenwood PL, Slepetis RM, Hermanson JW, Bell AW (1999) Intrauterine growth retardation is associated with reduced cell cycle activity, but not myofibre number, in ovine fetal muscle. Reproduction, Fertility and Development 11, 281–291.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Greenwood PL, Hunt AS, Hermanson JW, Bell AW (2000) Effects of birth weight and postnatal nutrition on neonatal sheep. II. Skeletal muscle growth and development. Journal of Animal Science 78, 50–61.
| PubMed |
open url image1

Greenwood PL, Davis J, Gaunt GM, Ferrier GR (2006a) Influences on the loin and cellular characteristics of the m. longissimus lumborum of Australian Poll Dorset-sired lambs. Australian Journal of Agricultural Research 57, 1–12.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Greenwood PL, Gardner GE, Hegarty RS (2006b) Lamb myofibre characteristics are influenced by sire estimated breeding values and pastoral nutritional system. Australian Journal of Agricultural Research 57, 627–639.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Hawkins RR, Moody WG, Kemp JD (1985) Influence of genetic type, slaughter weight and sex on ovine muscle fiber and fat-cell development. Journal of Animal Science 61, 1154–1163.
| PubMed |
open url image1

Hegarty RS, Shands C, Marchant R, Hopkins DL, Ball A, Harden S (2006a) Effects of available nutrition on the growth and muscling potential of sires on the development of crossbred lambs. 1. Growth and carcass characteristics. Australian Journal of Agricultural Research 57, 593–603.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Hegarty RS, Hopkins DL, Farrell T, Banks R, Harden S (2006b) Effects of available nutrition on the growth and muscling potential of sires on the development of crossbred lambs. 2. Composition and commercial yield. Australian Journal of Agricultural Research 57, 617–626.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Hopkins DL, Stanley DF, Martin LC, Gilmour AR (2007a) Genotype and age effects on sheep meat production. 1. Production and growth. Australian Journal of Experimental Agriculture 47, 1119–1127.
| Crossref |
open url image1

Hopkins DL, Stanley DF, Martin LC, Toohey ES, Gilmour AR (2007b) Genotype and age effects on sheep meat production. 3. Meat quality. Australian Journal of Experimental Agriculture 47, 1155–1164.
| Crossref |
open url image1

Kadim IT, Purchas RW, Davies AS, Rae AL, Barton RA (1993) Meat quality and muscle fibre type characteristics of Southdown rams from high and low backfat selection lines. Meat Science 33, 97–109.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Koohmaraie M, Shackelford SD, Wheeler TL, Lonergan SM, Doumit ME (1995) A muscle hypertrophy condition in lamb (Callipyge): characterization of effects on muscle growth and meat quality traits. Journal of Animal Science 73, 3596–3607.
| PubMed |
open url image1

Lorenzen CL, Koohmaraie M, Shackelford SD, Jahoor F, Freetly HC, Wheeler TL, Savell JW, Fiorotto ML (2000) Protein kinetics in Callipyge lambs. Journal of Animal Science 78, 78–87. open url image1

Maccatrozzo L, Patruno M, Toniolo L, Reggiani C, Mascarello F (2004) Myosin heavy chain 2B isoform is expressed in specialized eye muscles but not in trunk and limb muscles of cattle. European Journal of Histochemistry 48, 357–366. open url image1

Martin KM, Gardner GE, Thompson JM, Hopkins DL (2004) Nutritional impact on muscle glycogen metabolism in lambs selected for muscling. Journal of Animal and Feed Sciences 13(Suppl. 1), 639–642. open url image1

Moody WG, Tichenor DA, Kemp JD, Fox JD (1970) Effect of weight, castration and rate of gain on muscle fiber and fat cell diameter in two ovine muscles. Journal of Animal Science 31, 676–680. open url image1

Moody WG, Kemp JD, Mahyuddin M, Johnston DM, Ely DG (1980) Effect of feeding systems, slaughter weight and sex on histological properties of lamb carcasses. Journal of Animal Science 50, 249–256. open url image1

Nicastro F (1988) An improved procedure for identifying fibre types in ovine muscles. Meat Science 24, 73–78.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Peinado B, Latorre R, Vaquez-Auton JM, Poto A, Ramirez G, Lopez-Albors O, Moreno F, Gil F (2004) Histochemical skeletal muscle fibre types in the sheep. Anatomia, Histologia, Embryologia 33, 236–243.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Pethick DW, Harper GS, Oddy VH (2004) Growth, development and nutritional manipulation of marbling in cattle: a review. Australian Journal of Experimental Agriculture 44, 705–715.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Pethick DW, Warner RD, Banks RG (2006) Genetic improvement of lamb – industry issues and need for integrated research. Australian Journal of Agricultural Research 57, 591–592.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Picard B, Duris MP, Jurie C (1998) Classification of bovine muscle fibres by different histochemical techniques. The Histochemical Journal 30, 473–479.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Ponnampalam EN, Hopkins DL, Butler KL, Dunshea FR, Warner RD (2007) Genotype and animal age effects on sheep meat production. 2. Carcass quality traits. Australian Journal of Experimental Agriculture 47, 1147–1154. open url image1

Reggiani C, Mascarello F (2004) Fibre type identification and functional characterization in adult livestock animals. In ‘Muscle development of livestock animals. Physiology, genetics and meat quality’. (Eds MFW te Pas, ME Everts, HP Haagsman) pp. 39–68. (CAB International: Wallingford, UK)

Solomon MB, Moody WG, Kemp JD, Ely DG (1981) Effect of breed, slaughter weight and sex on histological properties of ovine muscle. Journal of Animal Science 52, 1019–1025.
| PubMed |
open url image1

Suzuki A (1973) Histochemical observations of individual skeletal muscle fibres in starved sheep. Japanese Journal of Zootechnical Science 44, 50–58. open url image1

Suzuki A, Cassens RG (1983) A histochemical study of myofiber types in the serratus ventralis thoracis muscle of sheep during growth. Journal of Animal Science 56, 1447–1458. open url image1

Vuocolo A, Byrne K, White J, McWilliam S, Reverter A, Cockett NE, Tellam RL (2007) Identification of a gene network contributing to hypertrophy in Callipyge skeletal muscle. Physiological Genomics 28, 253–272.
| Crossref |
open url image1

White NA, McGavin MD, Smith JE (1978) Age-related changes in percentage of fiber types and mean fiber diameters of the ovine quadriceps muscles. American Journal of Veterinary Research 39, 1297–1302.
| PubMed |
open url image1