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RESEARCH ARTICLE
Contents Vol 65(2)

Utilization of oocytes from closely related domestic species for interspecies somatic cell nuclear transfer in gaur embryo production

Ratree Jintana A , Kriengsak Tasripoo A , Kitiya Srisakwattana https://orcid.org/0000-0002-9184-0528 A * , Wanvipa Suthikrai A , Sunpetch Sophon B and Theerawat Tharasanit C
+ Author Affiliations
- Author Affiliations

A Research and Development Center for Livestock Production Technology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand.

B 3 Ramkamhaeng 118 alley 11-1, Ramkamhaeng Road, Saphansoong, Bangkok 10240, Thailand.

C Department of Obstetrics, Gynecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand.

* Correspondence to: skitiya@chula.ac.th

Handling Editor: Sathya Velmurugan

Animal Production Science 65, AN24218 https://doi.org/10.1071/AN24218
Submitted: 2 July 2024  Accepted: 17 December 2024  Published: 14 January 2025

© 2025 The Author(s) (or their employer(s)). Published by CSIRO Publishing

Abstract

Context

The limited availability of recipient oocytes poses significant challenges in increasing the population of endangered species.

Aims

This study demonstrates the use of oocytes from closely related domestic species (cattle, buffalo, and goat) in the production of gaur embryos via interspecies somatic cell nuclear transfer (iSCNT). The developmental abilities of embryos from various combinations – gaur–bovine, gaur–buffalo, gaur–goat, and others – are analyzed. Previous achievements are retrospectively reviewed and summarized.

Methods

The methodologies for iSCNT and in vitro gaur embryo production are outlined.

Key results

Blastocyst rates among gaur–bovine, gaur–buffalo, and gaur–goat embryos showed no significant differences (P < 0.05). Gaur–bovine embryo development was comparable to intraspecies embryos (bovine–bovine), indicating successful developmental compatibility. Pregnancy rates after transferring gaur–bovine embryos were reported for the first time, with rates of 4.76%, 42.86%, 19.05% and 4.76% at Days 45, 60, 90 and 120, respectively.

Conclusions

Bovine, buffalo, and goat oocyte cytoplasm is effective for producing gaur iSCNT embryos, capable of initiating early pregnancies.

Implications

Laboratory techniques and methodologies can save costs and time, contributing to the conservation of endangered species.

Keywords: bovine, buffalo, endangered species, gaur, goat, intergenus, interspecies somatic cell nuclear transfer, intersubspecies, intraspecies, livestock embryo.

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