In vitro fermentation characteristics of tropical legumes and grasses of good and poor nutritional quality and the degradability of their neutral detergent fibre
J. M. Castro-Montoya
A B , K. Goetz A and U. Dickhoefer A
+ Author Affiliations
- Author Affiliations
A University of Hohenheim, Institute of Agricultural Sciences in the Tropics (Hans-Ruthenberg-Institute), Animal Nutrition and Rangeland Management in the Tropics and the Subtropics, Fruwirthstrasse 31, 70599, Stuttgart, Germany.
B Corresponding author. Email jcm@uni-hohenheim.de
Animal Production Science 61(7) 645-654 https://doi.org/10.1071/AN20136
Submitted: 31 March 2020 Accepted: 15 December 2020 Published: 2 February 2021
Abstract
Context: Tropical legumes are commonly assumed to share all positive attributes known from temperate legumes such as lucerne. However, increasing evidence exists on the differences between those forages, particularly in terms of their ruminal degradability.
Aims: Exploring the 24-h rumen in vitro-fermentation characteristics of tropical legumes, their direct comparison with lucerne, and their interactions with grasses depending on their nutritional quality.
Methods: Arachis and stylosanthes (tropical legumes), pennisetum and andropogon (tropical grasses), and lucerne (lucerne_21 and lucerne_35, harvested 21 and 35 days after emergence respectively) were used for the study. On the basis of the nitrogen and neutral detergent fibre (NDF) concentration, arachis and pennisetum were classified as of good quality, while stylosanthes and andropogon as of poor quality. The following four incubation series were performed: first, forages alone were incubated under iso-nitrogenous conditions; second, forages were incubated under iso-nitrogenous conditions with supplemented starch; third, NDF extract of each forage was incubated alone; fourth, NDF extract of tropical grasses and legumes was incubated combined in grass : legume proportions of 33 : 67 and 67 : 33.
Key results: When incubated alone, gas production (GP) and total short chain fatty acids were higher for temperate legumes, intermediate for tropical legumes and lowest for tropical grasses. Similar trends were observed for GP when the forages were incubated with starch, but the differences between arachis and lucerne_35 disappeared; short chain fatty acids did not differ among all tropical forages. Moreover, acetate : propionate ratio was highest for tropical legumes, intermediate for temperate legumes, and lowest for tropical grasses. Gas production of NDF extracts was highest for the lucerne samples and lowest for the tropical legumes. Improvements in GP were found when the NDF from the poor-quality grass (andropogon) was combined with the legumes, particularly the good-quality legume (arachis).
Conclusions: On the basis of the gas production, tropical legumes appear to have lower degradability than do temperate ones, while also showing a different fermentation pattern. Fibre of tropical legumes is less degradable than that of tropical grasses, but when combining both fibre sources, there seems to be a synergistic effect on degradability.
Implications: The current results give important insights on the fermentation characteristics of tropical legumes, helping better understand their role in ruminants’ nutrition, while giving inputs towards improving their utilisation.
Keywords: in vitro digestibility, legumes, ruminal digestion.
References
Akin DE (1986) Interaction of ruminal bacteria and fungi with southern forages. Journal of Animal Science 63, 962–977.| Interaction of ruminal bacteria and fungi with southern forages.Crossref | GoogleScholarGoogle Scholar |
Åman P, Nordkvist E (1983) Chemical composition and in-vitro degradability of major chemical constituents of red clover harvested at different stages of maturity. Journal of the Science of Food and Agriculture 34, 1185–1189.
| Chemical composition and in-vitro degradability of major chemical constituents of red clover harvested at different stages of maturity.Crossref | GoogleScholarGoogle Scholar |
Ariza P, Bach A, Stern MD, Hall M (2001) Effects of carbohydrates from citrus pulp and hominy feed on microbial fermentation in continuous culture. Journal of Animal Science 79, 2713–2718.
| Effects of carbohydrates from citrus pulp and hominy feed on microbial fermentation in continuous culture.Crossref | GoogleScholarGoogle Scholar | 11721852PubMed |
Bailey RW, Ulyatt MJ (1970) Pasture quality and ruminant nutrition. New Zealand Journal of Agricultural Research 13, 591–604.
| Pasture quality and ruminant nutrition.Crossref | GoogleScholarGoogle Scholar |
Ben-Ghedalia D, Yosef E, Miron J, Est Y (1989) The effects of starch- and pectin-rich diets on quantitative aspects of digestion in sheep Animal Feed Science and Technology 24, 289–298.
| The effects of starch- and pectin-rich diets on quantitative aspects of digestion in sheepCrossref | GoogleScholarGoogle Scholar |
Blümmel M, Makkar HPS, Becker K (1997) In vitro gas production: a technique revisited. Journal of Animal Physiology and Animal Nutrition 77, 24–34.
| In vitro gas production: a technique revisited.Crossref | GoogleScholarGoogle Scholar |
Buxton D, Redfearn D (1997) Plant limitations to fiber digestion and utilization. The Journal of Nutrition 127, 814S–818S.
| Plant limitations to fiber digestion and utilization.Crossref | GoogleScholarGoogle Scholar | 9164243PubMed |
Castro-Montoya J, Dickhoefer U (2018) Effects of tropical legume silages on intake, digestibility and performance in large and small ruminants: a review. Grass and Forage Science 73, 26–39.
| Effects of tropical legume silages on intake, digestibility and performance in large and small ruminants: a review.Crossref | GoogleScholarGoogle Scholar |
Castro-Montoya J, Dickhoefer U (2020) The nutritional value of tropical legume forages fed to ruminants as affected by their growth habit and fed form: a systematic review. Animal Feed Science and Technology 269, 114641
| The nutritional value of tropical legume forages fed to ruminants as affected by their growth habit and fed form: a systematic review.Crossref | GoogleScholarGoogle Scholar |
D’Mello JPF (1992) Chemical constraints to the use of tropical legumes in animal nutrition. Animal Feed Science and Technology 38, 237–261.
| Chemical constraints to the use of tropical legumes in animal nutrition.Crossref | GoogleScholarGoogle Scholar |
da Silva TC, Pereira OG, Martins RM, Agarussi MCN, da Silva LD, Rufino LDA, Filho SC, Ribeiro KG (2018) Stylosanthes cv. Campo Grande silage and concentrate levels in diets for beef cattle. Animal Production Science 58, 539–545.
| Stylosanthes cv. Campo Grande silage and concentrate levels in diets for beef cattle.Crossref | GoogleScholarGoogle Scholar |
Dal Pizzol J, Ribeiro-Filho H, Quereuil A, Le Morvan A, Niderkorn V (2017) Complementarities between grasses and forage legumes from temperate and subtropical areas on in vitro rumen fermentation characteristics. Animal Feed Science and Technology 228, 178–185.
| Complementarities between grasses and forage legumes from temperate and subtropical areas on in vitro rumen fermentation characteristics.Crossref | GoogleScholarGoogle Scholar |
Dehority B, Johnson R, Conrad H (1962) Digestibility of forage hemicellulose and pectin by rumen bacteria in vitro and the effect of lignification thereon. Journal of Dairy Science 45, 508–512.
| Digestibility of forage hemicellulose and pectin by rumen bacteria in vitro and the effect of lignification thereon.Crossref | GoogleScholarGoogle Scholar |
Dewhurst R (2013) Milk production from silage: comparison of grass, legume and maize silages and their mixtures. Agricultural and Food Science 22, 57–69.
| Milk production from silage: comparison of grass, legume and maize silages and their mixtures.Crossref | GoogleScholarGoogle Scholar |
Diez-Gonzalez F, Bond D, Jennings E, Russell J (1999) Alternative schemes of butyrate production in Butyrivibrio fibrisolvens and their relationship to acetate utilization, lactate production, and phylogeny. Archives of Microbiology 171, 324–330.
| Alternative schemes of butyrate production in Butyrivibrio fibrisolvens and their relationship to acetate utilization, lactate production, and phylogeny.Crossref | GoogleScholarGoogle Scholar | 10382263PubMed |
France J, Dijkstra J (2005) Volatile fatty acid production. In ‘Quantitative aspects of ruminant digestion and metabolism 2’. (Eds J. Dijkstra, J.M. Forbes and J. France). pp. 157–175 (CAB International: Wallingford, Oxfordshire,UK)
Getachew G, Robinson P, DePeters E, Taylor S (2004) Relationships between chemical composition, dry matter degradation and in vitro gas production of several ruminant feeds. Animal Feed Science and Technology 111, 57–71.
| Relationships between chemical composition, dry matter degradation and in vitro gas production of several ruminant feeds.Crossref | GoogleScholarGoogle Scholar |
Gobbi K, Garcia R, Ventrella M (2011) Specific leaf area and quantitative leaf anatomy of signal grass and forage peanut submitted to shading. Brazilian Journal of Animal Science 40, 1436–1444.
Groot J, Williams B, Oostdam A, Boer H, Tamminga S (1998) The use of cumulative gas and volatile fatty acid production to predict in vitro fermentation kinetics of Italian ryegrass leaf cell walls and contents at various time intervals. British Journal of Nutrition 79, 519–525.
| The use of cumulative gas and volatile fatty acid production to predict in vitro fermentation kinetics of Italian ryegrass leaf cell walls and contents at various time intervals.Crossref | GoogleScholarGoogle Scholar |
Hunter R, McIntyre B, McIlroy R (1970) Water‐soluble carbohydrates of tropical pasture grasses and legumes. Journal of the Science of Food and Agriculture 21, 400–405.
| Water‐soluble carbohydrates of tropical pasture grasses and legumes.Crossref | GoogleScholarGoogle Scholar |
Kering MK, Guretzky J, Funderburg E, Mosali J (2011) Effect of nitrogen fertilizer rate and harvest season on forage yield, quality, and macronutrient concentrations in midland Bermuda grass. Communications in Soil Science and Plant Analysis 42, 1958–1971.
| Effect of nitrogen fertilizer rate and harvest season on forage yield, quality, and macronutrient concentrations in midland Bermuda grass.Crossref | GoogleScholarGoogle Scholar |
Lagasse MP, Goetsch AL, Landis KM, Forster LA (1990) Effects of supplemental alfalfa hay on feed intake and digestion by Holstein steers consuming high-quality bermudagrass or orchardgrass hay. Journal of Animal Science 68, 2839–2847.
| Effects of supplemental alfalfa hay on feed intake and digestion by Holstein steers consuming high-quality bermudagrass or orchardgrass hay.Crossref | GoogleScholarGoogle Scholar | 2211413PubMed |
Liu J, Pu Y, Xie Q, Wang J, Liu J (2015) Pectin induces an in vitro rumen microbial population shift attributed to the pectinolytic treponema group. Current Microbiology 70, 67–74.
| Pectin induces an in vitro rumen microbial population shift attributed to the pectinolytic treponema group.Crossref | GoogleScholarGoogle Scholar | 25178631PubMed |
Lüscher A, Mueller-Harvey I, Soussana JF, Rees RM, Peyraud JL (2014) Potential of legume-based grassland–livestock systems in Europe: a review. Grass and Forage Science 69, 206–228.
| Potential of legume-based grassland–livestock systems in Europe: a review.Crossref | GoogleScholarGoogle Scholar | 26300574PubMed |
Minson D (1990) ‘Forage in ruminant nutrition.’ (Academic Press Inc.: San Diego, CA, USA)
Niderkorn V, Baumont R (2009) Associative effects between forages on feed intake and digestion in ruminants. Animal 3, 951–960.
| Associative effects between forages on feed intake and digestion in ruminants.Crossref | GoogleScholarGoogle Scholar | 22444815PubMed |
Poppi D, McLennan S (1995) Protein and energy utilization by ruminants at pasture. Journal of Animal Science 73, 278–290.
| Protein and energy utilization by ruminants at pasture.Crossref | GoogleScholarGoogle Scholar | 7601744PubMed |
Russell J, Rychlik J (2001) Factors that alter rumen microbial ecology. Science 292, 1119–1122.
| Factors that alter rumen microbial ecology.Crossref | GoogleScholarGoogle Scholar | 11352069PubMed |
Salazar-Cubillas K, Dickhoefer U (2018) Validation of prediction equations to estimate rumen-undegradable crude protein in tropical feedstuffs using protein fractionation technique. In ‘Proceedings of the 10th International Symposium on the Nutrition of Herbivores.’ (Eds R Baumont, M Sildelberg, I Cassar-Malek) (Cambridge University Press: Cambridge, UK)
Silva A, Ørskov E (1988) Fibre degradation in the rumens of animals receiving hay, untreated or ammonia-treated straw. Animal Feed Science and Technology 19, 277–287.
| Fibre degradation in the rumens of animals receiving hay, untreated or ammonia-treated straw.Crossref | GoogleScholarGoogle Scholar |
Sturludóttir E, Brophy C, Bélanger G, Gustavsson AM, Jørgensen M, Lunnan T, Helgadóttir Á (2014) Benefits of mixing grasses and legumes for herbage yield and nutritive value in northern Europe and Canada. Grass and Forage Science 69, 229–240.
| Benefits of mixing grasses and legumes for herbage yield and nutritive value in northern Europe and Canada.Crossref | GoogleScholarGoogle Scholar |
Tiemann T, Lascano C, Kreuzer M, Hess H (2008) The ruminal degradability of fibre explains part of the low nutritional value and reduced methanogenesis in highly tanniniferous tropical legumes. Journal of the Science of Food and Agriculture 88, 1794–1803.
| The ruminal degradability of fibre explains part of the low nutritional value and reduced methanogenesis in highly tanniniferous tropical legumes.Crossref | GoogleScholarGoogle Scholar |
Udén P (2018) Fresh and ensiled forage plants: total composition, silage losses and the prediction of silage composition from the crop. Grass and Forage Science 73, 420–431.
| Fresh and ensiled forage plants: total composition, silage losses and the prediction of silage composition from the crop.Crossref | GoogleScholarGoogle Scholar |
Urdaneta AB, Fondevila M, Balcells J, Dapoza C, Castrillo C (2000) In vitro microbial digestion of straw cell wall polysaccharides in response to supplementation with different sources of carbohydrates Australian Journal of Agricultural Research 51, 393–399.
| In vitro microbial digestion of straw cell wall polysaccharides in response to supplementation with different sources of carbohydratesCrossref | GoogleScholarGoogle Scholar |
Van Soest P, Robertson J, Lewis B (1991) Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science 74, 3583–3597.
| Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition.Crossref | GoogleScholarGoogle Scholar | 1660498PubMed |
VDLUFA (1997) ‘Verband Deutscher Landwirtschaftlicher Untersuchungs- und Forschungsanstalten [Association of German Agricultural Investigation and Research Institutions]. Handbuch der Landwirtschaftlichen Versuchs- und Untersuchungsmethodik: (Methodenbuch III). Die chemische Untersuchung von Futtermitteln.’ (VDLUFA-Verlag: Darmstadt, Germany)
Weatherburn M (1967) Phenol–hypochlorite reaction for determination of ammonia. Analytical Chemistry 39, 971–974.
| Phenol–hypochlorite reaction for determination of ammonia.Crossref | GoogleScholarGoogle Scholar |
Wilson J, Hatfield R (1997) Structural and chemical changes of cell wall types during stem development: consequences for fibre degradation by rumen microflora. Australian Journal of Agricultural Research 48, 165–180.
| Structural and chemical changes of cell wall types during stem development: consequences for fibre degradation by rumen microflora.Crossref | GoogleScholarGoogle Scholar |
