Investigation of myostatin and calpain 3 gene polymorphisms and their association with milk-production traits in Sfakia sheep
Adel H. M. Ibrahim A D , Nikolaos Tzanidakis B , Smaragda Sotiraki B , Huitong Zhou C and Jonathan Hickford C
+ Author Affiliations
- Author Affiliations
A Department of Animal Breeding, Desert Research Center, 1 Matehaf AlMatariya Street, AlMatariya, Cairo 11753, Egypt.
B Veterinary Research Institute, Hellenic Agriculture Organisation, Thermi, TK 57001, Thessaloniki, Greece.
C Gene-Marker Laboratory, Department of Agricultural Sciences, Lincoln University, POB 84, Lincoln 7647, New Zealand.
D Corresponding author. Email: adelhosseiny2005@yahoo.com
Animal Production Science 60(3) 347-355 https://doi.org/10.1071/AN18799
Submitted: 16 January 2019 Accepted: 28 June 2019 Published: 3 December 2019
Abstract
Context: Genetic selection based on genetic markers for economically important traits in Sfakia sheep.
Aims: The aim of the present study was to investigate variation in the ovine myostatin gene (MSTN) and calpain 3 gene (CAPN3), and their association with milk-production traits.
Methods: Records for milk yield, milk fat content, protein content, lactose content, and non-fat solid content, pH and somatic-cell score (log), were obtained from 376 Sfakia ewes. Polymerase chain reaction–single-strand conformational polymorphism (PCR–SSCP) analyses were used to detect variation in intron 1 of MSTN and exon 10 of CAPN3. General linear models were then used to test for associations between the variation in MSTN and CAPN3, and milk-production traits.
Key results: The SSCP banding patterns for MSTN showed four variants (A1, A2, A3 and A4), which contained nine nucleotide sequence differences. Four SSCP banding patterns (C1, C2, C3 and C4) were observed for CAPN3 and these contained eight nucleotide-sequence differences. The MSTN variation was associated (P < 0.05) with variation in milk yield and non-fat milk solid content. Variation in CAPN3 was associated with milk yield (P < 0.001), fat content (P < 0.05) and lactose content (P < 0.05). Association analyses between the presence/absence of MSTN and CAPN3 variants and milk-production traits showed that a variant of MSTN that had previously between associated with muscle hypertrophy was associated with decreased milk yield (P < 0.05) and a lower non-fat milk solid content (P < 0.01). A CAPN3 variant that had previously been associated with increased sheep-carcass loin lean-meat yield was associated with a decreased milk yield (P < 0.01) and a decreased milk fat content (P < 0.05).
Conclusions: Our results have provided an insight into the effects of variation in ovine MSTN and CAPN3 on milk-production traits in sheep.
Implications: To preserve the dual-purpose characteristics of Sfakia sheep, breeding goals should take into account the possible antagonism between meat and milk traits.
Additional keywords: CAPN3, milk traits, MSTN.
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