Fatty acid composition and mRNA expression levels of lipid-metabolic genes in the muscles of ovariectomised young goats
Lei Zhang A , Yan-yan Wang A , Zhan-qin Zhou A B , Ming-zhe Fu A , Guang Li A , Fang Peng A and Lu Wan A
+ Author Affiliations
- Author Affiliations
A College of Animal Science and Technology, Northwest AandF University, Yangling, Shaanxi 712100, China.
B Corresponding author. Email: zhanqin_zhou@163.com
Animal Production Science 56(10) 1585-1592 https://doi.org/10.1071/AN14922
Submitted: 10 October 2014 Accepted: 13 April 2015 Published: 2 July 2015
Abstract
This study investigated the fatty acid composition and mRNA levels of acetyl-coenzyme A carboxylase (ACC), fatty acid synthase (FAS), hormone-sensitive lipase (HSL) and lipoprotein lipase (LPL) genes in the Longissimus dorsi muscle (LD) and Biceps femoris muscle (BF) of nine ovariectomised young goats, 50 days after ovariectomy at 5 months of age, relative to nine Control goats. Ovariectomy resulted in faster growth rates (130 vs 110 g/days; P = 0.027), more intramuscular fat (3.37% vs 1.62%; P = 0.009) and heavier final weights (28.3 vs 26.6 kg; P = 0.035). The total saturated fatty acid, C15:0 in the LD and C6:0, C11:0, C12:0, C15:0 and C21:0 in the BF were lower in ovariectomised goats than that in the Control goats (P < 0.05). Ovariectomy increased the total monounsaturated fatty acids in the LD (P < 0.05) and BF (P < 0.01). In the BF, the total polyunsaturated fatty acids and C18:2 of ovariectomised goats were lower than those of the Control goats (P < 0.05), and the C20:2 content significantly decreased in the LD (P < 0.05). The LD of the ovariectomised goats expressed significantly higher FAS but lower HSL mRNA levels than those of Control goats (P < 0.01). Significantly higher ACC, FAS and LPL and lower HSL mRNA levels were observed in the BF of the ovariectomised goats than in the BF of Controls (P < 0.05). These data suggest that ovariectomy affects fatty acid composition potentially by altering the expression of lipid-metabolic genes in the muscles of young goats.
Additional keywords: biceps femoris, Longissimus dorsi, ovariectomy, productivity performance.
References
Abu-Elheiga L, Matzuk MM, Abo-Hashema KA, Wakil SJ (2001) Continuous fatty acid oxidation and reduced fat storage in mice lacking acetyl-CoA carboxylase 2. Science 291, 2613–2616.| Continuous fatty acid oxidation and reduced fat storage in mice lacking acetyl-CoA carboxylase 2.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXisFCntLg%3D&md5=7f76da7d0bbb19e2e1e6b329c2780be3CAS | 11283375PubMed |
Alsted TJ, Ploug T, Prats C, Serup AK, Høeg L, Schjerling P, Holm C, Zimmermann R, Fledelius C, Galbo H (2013) Contraction‐induced lipolysis is not impaired by inhibition of hormone‐sensitive lipase in skeletal muscle. The Journal of Physiology 591, 5141–5155.
| Contraction‐induced lipolysis is not impaired by inhibition of hormone‐sensitive lipase in skeletal muscle.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXhs1SltrrN&md5=8d1215aca08485731563d1aab561332aCAS | 23878361PubMed |
Anderson SM, Rudolph MC, McManaman JL, Neville MC (2007) Secretory activation in the mammary gland: it is not just about milk protein synthesis. Breast Cancer Research 9, 204–217.
| Secretory activation in the mammary gland: it is not just about milk protein synthesis.Crossref | GoogleScholarGoogle Scholar | 17338830PubMed |
Bas P, Morand-Fehr P (2000) Effect of nutritional factors on fatty acid composition of lamb fat deposits. Livestock Production Science 64, 61–79.
| Effect of nutritional factors on fatty acid composition of lamb fat deposits.Crossref | GoogleScholarGoogle Scholar |
Bernert JT, Sprecher H (1975) Studies to determine the role rates of chain elongation and desaturation play in regulating the unsaturated fatty acid composition of rat liver lipids. Biochimica et Biophysica Acta (BBA) – Lipids and Lipid Metabolism 398, 354–363.
| Studies to determine the role rates of chain elongation and desaturation play in regulating the unsaturated fatty acid composition of rat liver lipids.Crossref | GoogleScholarGoogle Scholar |
Cameron N, Enser M (1991) Fatty acid composition of lipid in Longissimus dorsi muscle of Duroc and British Landrace pigs and its relationship with eating quality. Meat Science 29, 295–307.
| Fatty acid composition of lipid in Longissimus dorsi muscle of Duroc and British Landrace pigs and its relationship with eating quality.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK3MXmsFCmuro%3D&md5=54c014aa2694419c912834625cc703aaCAS | 22061434PubMed |
Cameron N, Enser M, Nute G, Whittington F, Penman J, Fisken A, Perry A, Wood J (2000) Genotype with nutrition interaction on fatty acid composition of intramuscular fat and the relationship with flavour of pig meat. Meat Science 55, 187–195.
| Genotype with nutrition interaction on fatty acid composition of intramuscular fat and the relationship with flavour of pig meat.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3cXit1yrs7s%3D&md5=9297d1cb6b9688880ad073b71002ff3dCAS | 22061084PubMed |
Estrada-Camarena E, López-Rubalcava C, Hernández-Aragón A, Mejía-Mauries S, Picazo O (2011) Long-term ovariectomy modulates the antidepressant-like action of estrogens, but not of antidepressants. Journal of Psychopharmacology (Oxford, England) 25, 1365–1377.
| Long-term ovariectomy modulates the antidepressant-like action of estrogens, but not of antidepressants.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXhs1Wgt7zL&md5=c7f6ba56453918d5274f03496a1a2198CAS |
Frye CA, Walf AA (2002) Changes in progesterone metabolites in the hippocampus can modulate open field and forced swim test behavior of proestrous rats. Hormones and Behavior 41, 306–315.
| Changes in progesterone metabolites in the hippocampus can modulate open field and forced swim test behavior of proestrous rats.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38XktVGhsrw%3D&md5=835aa69de99af6d8a09050946effed77CAS | 11971664PubMed |
Fung TT, Rexrode KM, Mantzoros CS, Manson JE, Willett WC, Hu FB (2009) Mediterranean diet and incidence of and mortality from coronary heart disease and stroke in women. Circulation 119, 1093–1100.
| Mediterranean diet and incidence of and mortality from coronary heart disease and stroke in women.Crossref | GoogleScholarGoogle Scholar | 19221219PubMed |
Gill S, Sharpless JL, Rado K, Hall JE (2002) Evidence that GnRH decreases with gonadal steroid feedback but increases with age in postmenopausal women. The Journal of Clinical Endocrinology and Metabolism 87, 2290–2296.
| Evidence that GnRH decreases with gonadal steroid feedback but increases with age in postmenopausal women.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38XjsFGhu7k%3D&md5=6b9c7f4ee3ef0115edbfecbcf227410fCAS | 11994378PubMed |
Goldberg IJ (1996) Lipoprotein lipase and lipolysis: central roles in lipoprotein metabolism and atherogenesis. Journal of Lipid Research 37, 693–707.
Holm C (2003) Molecular mechanisms regulating hormone-sensitive lipase and lipolysis. Biochemical Society Transactions 31, 1120–1124.
| Molecular mechanisms regulating hormone-sensitive lipase and lipolysis.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXps1yqu74%3D&md5=655f3d49e2244dbe00a1a19b51097ad1CAS | 14641008PubMed |
Johnson R, Gilbert J, Cooper R, Dai X, Newton B, Tracy R, West W, DeMoss T, Myers P, Streckfus C (1997) Alveolar bone loss one year following ovariectomy in sheep. Journal of Periodontology 68, 864–871.
| Alveolar bone loss one year following ovariectomy in sheep.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DyaK1c%2FitFWitg%3D%3D&md5=fb29ea4629aea4dd79570331a034f951CAS | 9379331PubMed |
Jones AP, McElroy JF, Crnic L, Wade GN (1991) Effects of ovariectomy on thermogenesis in brown adipose tissue and liver in Syrian hamsters. Physiology & Behaviour 50, 41–45.
Karsch FJ, Dahl GE, Evans NP, Manning JM, Mayfield KP, Moenter SM, Foster DL (1993) Seasonal changes in gonadotropin-releasing hormone secretion in the ewe: alteration in response to the negative feedback action of estradiol. Biology of Reproduction 49, 1377–1383.
| Seasonal changes in gonadotropin-releasing hormone secretion in the ewe: alteration in response to the negative feedback action of estradiol.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2cXosFw%3D&md5=5a9e5bb4e279d0e11bf0af05ceac1679CAS | 8286620PubMed |
Kersten S (2014) Physiological regulation of lipoprotein lipase. Biochimica Et Biophysica Acta - Molecular and Cell Biology of Lipids 1841, 919–933.
| Physiological regulation of lipoprotein lipase.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2cXnvFSrsrk%3D&md5=5ff5ac1731eda8327f8daf27b93b7c5aCAS |
Kim Y, Lee M, Lim Y, Jang Y, Park HK, Lee Y (2013) The gene–diet interaction, LPL PvuII and HindIII and carbohydrate, on the criteria of metabolic syndrome: KMSRI-Seoul study. Nutrition (Burbank, Los Angeles County, Calif.) 29, 1115–1121.
| The gene–diet interaction, LPL PvuII and HindIII and carbohydrate, on the criteria of metabolic syndrome: KMSRI-Seoul study.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXht1eltLvN&md5=de6cb4b5c86fb7037246c07496036054CAS |
Kimata M, Ishibashi T, Kamada T (2001) Studies on relationship between sensory evaluation and chemical composition in various breeds of pork. Japanese Journal of Swine Science 38, 45–51.
| Studies on relationship between sensory evaluation and chemical composition in various breeds of pork.Crossref | GoogleScholarGoogle Scholar |
Liang DM, Wang JC, Li Y (2007) The effects of androgen and estradiol on blood lipid metabolism and the correlated enzyme of hepatic blood lipid metabolism in male rats. Chinese Journal of Gerontology 27, 1121–1124.
Loftus TM, Jaworsky DE, Frehywot GL, Townsend CA, Ronnett GV, Lane MD, Kuhajda FP (2000) Reduced food intake and body weight in mice treated with fatty acid synthase inhibitors. Science 288, 2379–2381.
| Reduced food intake and body weight in mice treated with fatty acid synthase inhibitors.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3cXkslWitb0%3D&md5=63b67735c200300a2eaa5316d8eda92eCAS | 10875926PubMed |
Louca A, Economides S, Hancock J (1977) Effects of castration on growth rate, feed conversion efficiency and carcass quality in Damascus goats. Animal Production 24, 387–391.
| Effects of castration on growth rate, feed conversion efficiency and carcass quality in Damascus goats.Crossref | GoogleScholarGoogle Scholar |
Mackay J, Pearce M, Thevasagayam S, Doran O (2013) Fatty acid composition and lipogenic enzyme protein expression in subcutaneous adipose tissue of male pigs vaccinated against boar taint, barrows, and entire boars. Journal of Animal Science 91, 395–404.
| Fatty acid composition and lipogenic enzyme protein expression in subcutaneous adipose tissue of male pigs vaccinated against boar taint, barrows, and entire boars.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXjsFKhs78%3D&md5=1f552d5c1590c06e68e12f0d655c3310CAS | 23048133PubMed |
Mascarenhas R, Simões J (2007) Laparoscopically aided ovariectomy in goats. In ‘Reproduction in domestic animals’. p. 137. (Blackwell Publishing: Oxford)
McElroy JF, Wade GN (1987) Short-and long-term effects of ovariectomy on food intake, body weight, carcass composition, and brown adipose tissue in rats. Physiology & Behavior 39, 361–365.
| Short-and long-term effects of ovariectomy on food intake, body weight, carcass composition, and brown adipose tissue in rats.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL2sXhtlWqtr0%3D&md5=22e982f652b0a70c5fc2bd87c87adca9CAS |
Menendez JA, Lupu R (2007) Fatty acid synthase and the lipogenic phenotype in cancer pathogenesis. Nature Reviews. Cancer 7, 763–777.
| Fatty acid synthase and the lipogenic phenotype in cancer pathogenesis.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXhtVOnsr3P&md5=3b80f7b6eb20f8ed95619fb33fda1be1CAS | 17882277PubMed |
Nogalska A, Swierczynski J (2001) The age-related differences in obese and fatty acid synthase gene expression in white adipose tissue of rat. Biochimica et Biophysica Acta (BBA) – Molecular and Cell Biology of Lipids 1533, 73–80.
| The age-related differences in obese and fatty acid synthase gene expression in white adipose tissue of rat.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXmtFakurc%3D&md5=51201fc2efcdb6d44d2bfe2085600d82CAS |
Ogasawara J, Nomura S, Rahman N, Sakurai T, Kizaki T, Izawa T, Ishida H, Haga S, Ohno H (2010) Hormone-sensitive lipase is critical mediators of acute exercise-induced regulation of lipolysis in rat adipocytes. Biochemical and Biophysical Research Communications 400, 134–139.
| Hormone-sensitive lipase is critical mediators of acute exercise-induced regulation of lipolysis in rat adipocytes.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXhtFGju73I&md5=112001ce1c1400dab5002866ac642e6eCAS | 20708600PubMed |
Oliveira PWC, De Sousa GJ, Caliman IF, Lamas AZ, De Medeirost ARS, De Andradm TU, Abreu GRB, De Figueiredo SG, Bissoli NS (2014) Metformin ameliorates ovariectomy-induced vascular dysfunction in non-diabetic Wistar rats. Clinical Science 127, 265–275.
| Metformin ameliorates ovariectomy-induced vascular dysfunction in non-diabetic Wistar rats.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2cXns12iurc%3D&md5=2896169f7f1f0c088827bac7e87f1dbeCAS |
Pizer ES, Lax SF, Kuhajda FP, Pasternack GR, Kurman RJ (1998) Fatty acid synthase expression in endometrial carcinoma. Cancer 83, 528–537.
| Fatty acid synthase expression in endometrial carcinoma.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1cXlt1agtbs%3D&md5=cf5e228c2a4789125ed4bdd02a36579bCAS | 9690546PubMed |
Shi H, Luo J, Yao D, Zhu J, Xu H, Shi H, Loor J (2013) Peroxisome proliferator-activated receptor-γ stimulates the synthesis of monounsaturated fatty acids in dairy goat mammary epithelial cells via the control of stearoyl-coenzyme A desaturase. Journal of Dairy Science 96, 7844–7853.
| Peroxisome proliferator-activated receptor-γ stimulates the synthesis of monounsaturated fatty acids in dairy goat mammary epithelial cells via the control of stearoyl-coenzyme A desaturase.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXhs1Whtr7M&md5=b266eb050cdf7d7a2547ab4f8d8b47f1CAS | 24119817PubMed |
Siebert C, Kolling J, Scherer EBS, Schmitz F, da Cunha MJ, Mackedanz V, de Andrade RB, Wannmacher CMD, Wyse ATS (2014) Effect of physical exercise on changes in activities of creatine kinase, cytochrome c oxidase and ATP levels caused by ovariectomy. Metabolic Brain Disease 29, 825–835.
| Effect of physical exercise on changes in activities of creatine kinase, cytochrome c oxidase and ATP levels caused by ovariectomy.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2cXnvFekur8%3D&md5=0231690abeb0f550fe67017823c6ed17CAS | 24810635PubMed |
Sigrist IM, Gerhardt C, Alini M, Schneider E, Egermann M (2007) The long-term effects of ovariectomy on bone metabolism in sheep. Journal of Bone and Mineral Metabolism 25, 28–35.
| The long-term effects of ovariectomy on bone metabolism in sheep.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XhtlGitbbO&md5=f40bccaf1c3c9cd219a70f55fa352910CAS | 17187191PubMed |
Soper RJ, Oguz C, Emery R, Pitsillides AA, Hodges SJ (2014) Vitamin K catabolite inhibition of ovariectomy-induced bone loss: structure-activity relationship considerations. Molecular Nutrition & Food Research 58, 1658–1666.
| Vitamin K catabolite inhibition of ovariectomy-induced bone loss: structure-activity relationship considerations.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2cXhtFCkurvK&md5=d012ce3424ec577027505abdcc233aadCAS |
Tanos R, Murray IA, Smith PB, Patterson A, Perdew GH (2012) Role of the ah receptor in homeostatic control of fatty acid synthesis in the liver. Toxicological Sciences 129, 372–379.
| Role of the ah receptor in homeostatic control of fatty acid synthesis in the liver.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38XhsVSms7%2FN&md5=6195e2922fcef62baa297dda689a0c61CAS | 22696238PubMed |
Vagelos PR, Alberts A, Martin DB (1963) Studies on the mechanism of activation of acetyl coenzyme A carboxylase by citrate. The Journal of Biological Chemistry 238, 533–540.
Vaughan M, Berger JE, Steinberg D (1964) Hormone-sensitive lipase and monoglyceride lipase activities in adipose tissue. The Journal of Biological Chemistry 239, 401–409.
Vega-Rivera NM, Fernandez-Guasti A, Ramirez-Rodriguez G, Estrada-Camarena E (2013) Acute stress further decreases the effect of ovariectomy on immobility behavior and hippocampal cell survival in rats. Psychoneuroendocrinology 38, 1407–1417.
| Acute stress further decreases the effect of ovariectomy on immobility behavior and hippocampal cell survival in rats.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXhtFGqu7g%3D&md5=a87dc9c99932f3557cb5ab771cfe3053CAS | 23333251PubMed |
Villalobos-Villalobos G, González-Rios H, Núñez-González FA, Domínguez-Díaz D, Peña-Ramos EA, Huerta-Leidenz N (2013) Effects of castration and zeranol on fatty acid composition and cholesterol content of hair lamb meat. Journal of Applied Animal Research 42, 65–72.
| Effects of castration and zeranol on fatty acid composition and cholesterol content of hair lamb meat.Crossref | GoogleScholarGoogle Scholar |
Wade GN (1972) Gonadal hormones and behavioral regulation of body weight. Physiology & Behavior 8, 523–534.
| Gonadal hormones and behavioral regulation of body weight.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaE38XksF2gsro%3D&md5=a486cf78a8b4e927384cb8a07f252fd5CAS |
Wade GN, Gray JM (1978) Cytoplasmic 17β-[3H] estradiol binding in rat adipose tissues. Endocrinology 103, 1695–1701.
| Cytoplasmic 17β-[3H] estradiol binding in rat adipose tissues.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaE1MXjsVWlsA%3D%3D&md5=5e6fc2dbf083c3e9912bccd467de84acCAS | 748011PubMed |
Waite M, Wakil SJ (1962) Studies on the mechanism of fatty acid synthesis XII. Acetyl Coenzyme A carboxylase. The Journal of Biological Chemistry 237, 2750–2757.
Wakil SJ (1989) Fatty acid synthase, a proficient multifunctional enzyme. Biochemistry 28, 4523–4530.
| Fatty acid synthase, a proficient multifunctional enzyme.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL1MXit1OntLw%3D&md5=7e2a01d1892a88330e985daad5b3705aCAS | 2669958PubMed |
Yang YH, Zheng XF, Li B, Jiang SD, Jiang LS (2014) Increased activity of osteocyte autophagy in ovariectomized rats and its correlation with oxidative stress status and bone loss. Biochemical and Biophysical Research Communications 451, 86–92.
| Increased activity of osteocyte autophagy in ovariectomized rats and its correlation with oxidative stress status and bone loss.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2cXht1ClurfP&md5=ced5bdcf67eb7dff41838d0ff207f130CAS |
Yao YC (2008) Studies on the developmental expression of the genes involved in intramuscular adipocyte differentiation and of key enzymes involved in intramuscular fat metabolism in Sunit lambs. PhD Thesis, Inner Mongolia Agricultural University, Huhehot, China.
Zhang L, Wang Y-y, Fu M-z, Li G, An N, Li S-y, Zhou Z-q (2014) The effects of ovariectomy on meat performance and expression of GH/IGF-I in young goats. Canadian Journal of Animal Science 94, 619–626.
| The effects of ovariectomy on meat performance and expression of GH/IGF-I in young goats.Crossref | GoogleScholarGoogle Scholar |
Zhu XC, Lin J, Wang Q, Liu H, Qiu L, Fang DZ (2014a) Associations of lipoprotein lipase gene rs326 with changes of lipid profiles after a high-carbohydrate and low-fat diet in healthy Chinese Han youth. International Journal of Environmental Research and Public Health 11, 4544–4554.
| Associations of lipoprotein lipase gene rs326 with changes of lipid profiles after a high-carbohydrate and low-fat diet in healthy Chinese Han youth.Crossref | GoogleScholarGoogle Scholar | 24762672PubMed |
Zhu HJ, Wang LJ, Wang XQ, Pan H, Li NS, Yang HB, Jin M, Zang BX, Gong FY (2014b) Hormone-sensitive lipase is involved in the action of hydroxysafflor yellow A (HYSA) inhibiting adipogenesis of 3T3–L1 cells. Fitoterapia 93, 182–188.
| Hormone-sensitive lipase is involved in the action of hydroxysafflor yellow A (HYSA) inhibiting adipogenesis of 3T3–L1 cells.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2cXislOju70%3D&md5=e18a19e468101607a40d20faea3955b9CAS | 24440907PubMed |
