Transfer of aflatoxins from naturally contaminated feed to milk of Nili-Ravi buffaloes fed a mycotoxin binder
N. Aslam A E , I. Rodrigues B , D. M. McGill A C , H. M. Warriach A C , A. Cowling A , A. Haque D and P. C. Wynn AA Graham Centre for Agricultural Innovation, Charles Sturt University, Wagga Wagga, 2650 NSW, Australia.
B BIOMIN Holding GmbH, Herzongenburg, Austria.
C ASLP Dairy Project, University of Veterinary and Animal Sciences, Lahore 54000, Pakistan.
D Buffalo Research Institute (BRI), Pattoki district Kasur, Pakistan.
E Corresponding author. Email: naslam@csu.edu.au; drnaveedaslam@gmail.com
Animal Production Science 56(10) 1637-1642 https://doi.org/10.1071/AN14909
Submitted: 28 October 2014 Accepted: 6 March 2015 Published: 16 June 2015
Abstract
The objectives of this study were to observe the extent of transfer of aflatoxin B1 in feed to the aflatoxin M1 metabolite in milk in Nili-Ravi buffaloes and to evaluate the efficacy of a commercial mycotoxin binder (Mycofix, Biomin Singapore) incorporated into feed to minimise this transfer. Multiparous animals (n = 28) were randomly distributed to four groups corresponding to two treatments each with two levels of aflatoxin B1. Individual animals were exposed to naturally contaminated feed providing a total of 1475 µg/day (Groups A and B) or 2950 µg/day (Groups C and D) of aflatoxin B1. Groups B and D were given 50 g of mycotoxin binder daily mixed with feed whereas Groups A and C were kept as controls. Feed samples were analysed by reverse phase high performance liquid chromatography for aflatoxin B1 and milk samples were evaluated by enzyme-linked immunosorbent assay for the liver metabolite aflatoxin M1. The mean value of total daily aflatoxin M1 excretion for animals fed 2950 µg/day of aflatoxin B1 (112.6 µg/day) was almost double (P < 0.001) than the excretion in buffaloes fed 1475 µg/day (62.2 µg/day). The mean daily concentration of aflatoxin M1 in milk of animals from both treatment groups supplemented with 50 g/day of mycotoxin binder was 76.5 µg/day, nearly 22 µg lower than those without binder at 98.3 µg/day (s.e.d. = 5.99: P < 0.01). The interaction of binder and treatment was not significant i.e. the 50 g/day of binder was able to sequester aflatoxin B1 with the same efficiency in groups fed with high and low concentrations of aflatoxin B1. Carry over was (3.44%) lower (P = 0.001) in animals supplemented with 50 g/day of mycotoxin binder than those fed no binder (4.60%). Thus buffaloes are highly efficient at transferring aflatoxins in feed to the aflatoxin M1 metabolite in milk, whereas mycotoxin binder is capable of alleviating without preventing this contamination risk.
Additional keywords: AFB1, AFM1, mycotoxin binder, Nili-Ravi buffaloes, transfer.
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