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RESEARCH ARTICLE
Contents Vol 49(4)

Quantitative analysis of performance, carcass and meat quality traits in cattle from two Australian beef herds in which a null myostatin allele is segregating

B. A. O’ Rourke A B G , J. A. Dennis B E , P. J. Healy B E , W. A. McKiernan A C , P. L. Greenwood A D , L. M. Cafe A D , D. Perry A D , K. H. Walker B F , I. Marsh B , P. F. Parnell A D and P. F. Arthur A B
+ Author Affiliations
- Author Affiliations

A Cooperative Research Centre for Beef Genetic Technologies, University of New England, Armidale, NSW 2351, Australia.

B New South Wales Department of Primary Industries, Elizabeth Macarthur Agricultural Institute, Camden, NSW 2570, Australia.

C New South Wales Department of Primary Industries, Orange Agricultural Institute, Orange, NSW 2800, Australia.

D New South Wales Department of Primary Industries, Beef Industry Centre of Excellence, University of New England, Armidale, NSW 2351, Australia.

E Hereford Hall Road, Braidwood, NSW 2622, Australia.

F Meat and Livestock Australia, Level 1, 165 Walker Street, North Sydney, NSW 2060, Australia.

G Corresponding author. Email: brendon.orourke@dpi.nsw.gov.au

Animal Production Science 49(4) 297-305 https://doi.org/10.1071/EA08206
Submitted: 23 July 2008  Accepted: 16 January 2009   Published: 6 April 2009

Abstract

Two Australian beef cattle herds, in which selection for muscularity was a primary objective, were used in this study to identify bovine myostatin (MSTN) mutations associated with increased muscling, and to assess associations between genotype and performance, carcass and meat quality traits. One was a research herd (herd A) established from Angus × Hereford cows, and comprised a high and low muscle selection line. The other (herd B) was a commercial beef enterprise with cattle of Angus and Charolais origin. Sequencing of the MSTN coding region and flanking splice junctions in an initial sample of 34 animals from both herds identified the 821 del11 mutation as well as six other polymorphic sites. The nucleotide 374–50C > T polymorphism in intron 1 was found to be in linkage disequilibrium with the 821 del11 mutation, with both variants confined to the high muscle selection line in herd A. No other variants were exclusive to either of the two herd A selection lines. The effect of the 821 del11 mutation was further investigated in a total of 803 cattle from both herds. A relatively high prevalence of 821 del11 heterozygotes (herd A 16%; herd B 23%) was found and heterozygotes had significant advantages in eye muscle area and muscle score over their wildtype counterparts, and did not differ in meat quality. Retail beef yield from steers was higher for the 821 del11 heterozygotes from herd A (67.0 v. 63.5%) and herd B (71.8 v. 68.6%), relative to homozygous wildtype contemporaries, demonstrating the benefits of incorporating single null MSTN alleles into breeding programs.


Acknowledgements

This study was funded by the NSW Department of Primary Industries and Meat and Livestock Australia. We thank the commercial herd (herd B) owner B. Brooker for the use of his herd and records. The assistance provided by the following NSW DPI staff is gratefully appreciated: Phil Dawes, Peter Kamphorst, Peter Newman, Stuart McClelland, Joe Brunner and Bill Johns. Additional technical support was provided by Matt Wolcott from the Animal Genetics and Breeding Unit, and Jason Siddell, Department of Meat Science, University of New England. The excellent cooperation of the management and staff of John Dee Abattoir, Warwick, and associated Yarranbrook feedlot, Inglewood, in particular Warren Stiff, Geoff Grant, Peter Healy and John Calvert, is also gratefully acknowledged.


References


Arthur PF (1995) Double muscling in cattle: a review. Australian Journal of Agricultural Research 46, 1493–1515.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Arthur PF, Makarechian M, Price MA, Berg RT (1989a) Heterosis, maternal and direct effects in double-muscled and normal cattle: I. Reproduction and growth traits. Journal of Animal Science 67, 902–910.
|  CAS | PubMed |
open url image1

Arthur PF, Makarechian M, Price MA, Berg RT (1989b) Heterosis, maternal and direct effects in double-muscled and normal cattle: II. Carcass traits of young bulls. Journal of Animal Science 67, 911–919.
|  CAS | PubMed |
open url image1

AUS-MEAT (1998) ‘Handbook of Australian meat.’ (AUS-MEAT Ltd: Brisbane)

Bailey AJ, Enser MB, Dransfield E, Restall DJ, Avery NC (1982) ‘Muscle and adipose tissue from normal and double muscled cattle: collagen types, muscle fibre diameter, fat cell size and fatty acid composition and organoleptic properties.’ (Martinus Nijhoff Publishers: The Hague)

Cappucio I, Martichelli C, Serracchioli A, Nardone A, Filippini F, Ajmone-Marsan P, Valentini A (1998) A G-T transversion introduces a stop codon at the mh locus in hypertrophic Marchigiana beef subjects. Animal Genetics 29(Suppl.), 51. open url image1

Casas E, Keele JW, Shackelford SD, Koohmaraie M, Sonstegard TS, Smith TP, Kappes SM, Stone RT (1998) Association of the muscle hypertrophy locus with carcass traits in beef cattle. Journal of Animal Science 76, 468–473.
|  CAS | PubMed |
open url image1

Casas E, Keele JW, Fahrenkrug SC, Smith TP, Cundiff LV, Stone RT (1999) Quantitative analysis of birth, weaning, and yearling weights and calving difficulty in Piedmontese crossbreds segregating an inactive myostatin allele. Journal of Animal Science 77, 1686–1692.
|  CAS | PubMed |
open url image1

Casas E, Bennett GL, Smith TP, Cundiff LV (2004) Association of myostatin on early calf mortality, growth, and carcass composition traits in crossbred cattle. Journal of Animal Science 82, 2913–2918.
|  CAS | PubMed |
open url image1

Clop A, Marcq F, Takeda H, Pirottin D, Tordoir X, Bibé B, Bouix J, Caiment F, Elsen J-M, Eychenne F, Larzul C, Laville E, Meish F, Milenkovic D, Tobin J, Charlier C, Georges M (2006) A mutation creating a potential illegitimate microRNA target site in the myostatin gene affects muscularity in sheep. Nature Genetics 38, 813–818.
| Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1

Crisa A, Marchitelli C, Savarese MC, Valentini A (2003) Sequence analysis of myostatin promoter in cattle. Cytogenetic and Genome Research 102, 48–52.
| Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1

Dennis JA, Healy PJ (2001) Genotyping Shorthorn cattle for generalised glycogenosis. Australian Veterinary Journal 79, 773–775.
| Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1

Dunner S, Miranda ME, Amigues Y, Canon J, Georges M, Hanset R, Williams J, Menissier F (2003) Haplotype diversity of the myostatin gene among beef cattle breeds. Genetics, Selection, Evolution 35, 103–118.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Gill JL, Bishop SC, McCorquodale C, Williams JL, Wiener P (2009) Associations between the 11-bp deletion in the myostatin gene and carcass quality in Angus-sired cattle. Animal Genetics 40, 97–100.
|  CAS | Crossref | PubMed |
open url image1

Grobet L, Martin LJ, Poncelet D, Pirottin D, Brouwers B, Riquet J, Schoeberlein A, Dunner S, Ménissier F, Massabanda J, Fries R, Hanset R, Georges M (1997) A deletion in the bovine myostatin gene causes the double-muscled phenotype in cattle. Nature Genetics 17, 71–74.
| Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1

Grobet L, Poncelet D, Royo LJ, Brouwers B, Pirottin D, Michaux C, Ménissier F, Zanotti M, Dunner S, Geroges M (1998) Molecular definition of an allelic series of mutations disrupting the myostatin function and causing double-muscling in cattle. Mammalian Genome 9, 210–213.
| Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1

Hanset R (1991) ‘The major gene of muscular hypertrophy in the Belgian Blue cattle breed.’ (CAB International: Wallingford, UK)

Kambadur R, Sharma M, Smith TP, Bass JJ (1997) Mutations in myostatin (GDF8) in double-muscled Belgian Blue and Piedmontese cattle. Genome Research 7, 910–916.
|  CAS | PubMed |
open url image1

Levéziel H, Hocquette JF, Lepetit J, Denoyelle C, Dodelin V, Payet N, Malafosse A, Renand G (2006) Q204X myostatin mutation effects on carcass and meat quality traits in heterozygous Charolais young bulls. In ‘52nd international congress of meat science and technology’. pp. 65–66. (Meat Science)

McKiernan WA (1990) New developments in live animal appraisal of meat quantity in beef cattle. Proceedings of the Australian Association of Animal Breeding and Genetics 9, 447–450. open url image1

McPherron AC, Lee SJ (1997) Double muscling in cattle due to mutations in the myostatin gene. Proceedings of the National Academy of Sciences of the United States of America 94, 12 457–12 461.
| Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1

McPherron AC, Lawler AM, Lee SJ (1997) Regulation of skeletal muscle mass in mice by a new TGF-beta superfamily member. Nature 387, 83–90.
| Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1

Mosher DS, Quignon P, Bustamante CD, Sutter NB, Mellersh CS, Parker HG, Ostrander EA (2007) A mutation in the myostatin gene increases muscle mass and enhances racing performance in heterozygote dogs. PLOS Genetics 3, e79.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Payne RW, Harding SA, et al (2007) ‘GENSTAT release 10. Reference manual. Part 1: summary.’ (VSN International: Hemel Hempstead, UK)

Perry D, Shorthose WR, Ferguson DM, Thompson JM (2001) Methods used in the CRC program for the determination of carcass yield and beef quality Australian Journal of Experimental Agriculture 41, 953–957.
| Crossref | GoogleScholarGoogle Scholar | open url image1

SAS Institute (2001) ‘SAS user’s guide. Version 9.1.’ (SAS Institute Inc.: Cary, NC)

Schuelke M, Wagner KR, Stolz LE, Hubner C, Riebel T, Komen W, Braun T, Tobin JF, Lee SJ (2004) Myostatin mutation associated with gross muscle hypertrophy in a child. The New England Journal of Medicine 350, 2682–2688.
| Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1

Sellick GS, Pitchford WS, Morris CA, Cullen NG, Crawford AM, Raadsma HW, Bottema CD (2007) Effect of myostatin F94L on carcass yield in cattle. Animal Genetics 38, 440–446.
| Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1

Short RE, MacNeil MD, Grosz MD, Gerrard DE, Grings EE (2002) Pleitropic effects in Hereford, Limousin, and Piedmontese F2 crossbred calves of genes controlling muscularity including Piedmontese myostatin allele. Journal of Animal Science 80, 1–11.
|  CAS | PubMed |
open url image1

Smith JA, Lewis AM, Wiener P, Williams JL (2000) Genetic variation in the bovine myostatin gene in UK beef cattle: allele frequencies and haplotype analysis in the South Devon. Animal Genetics 31, 306–309.
| Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1

Stephens M, Scheet P (2005) Accounting for decay of linkage disequilibrium in haplotype inference and missing-data imputation. American Journal of Human Genetics 76, 449–462.
| Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1

Stephens M, Smith NJ, Donnelly P (2001) A new statistical method for haplotype reconstruction from population data. American Journal of Human Genetics 68, 978–989.
| Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1

Swatland HJ, Kieffer NM (1974) Fetal development of the double muscled condition in cattle. Journal of Animal Science 38, 752–757.
|  CAS | PubMed |
open url image1

Tatum JD, Gronewald KW, Seideman SC, Lamm WD (1990) Composition and quality of beef from steers sired by Piedmontese, Gelbvieh and Red Angus bulls. Journal of Animal Science 68, 1049–1060. open url image1

Uytterhaegen L, Claeys E, Demeyer D (1994) Effects of exogenous protease effectors on beef tenderness development and myofibrillar degradation and solubility. Journal of Animal Science 72, 1209–1223.
|  CAS | PubMed |
open url image1

Vissac B, Ménissier F, Perreau B (1973) Etude du caractère culard. VII. Croissance et musculature des femelles déséquilibre morphologique an vêlage. Annales de Genetique et de Selection Animale 5, 23–38.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Wheeler TL, Shackelford SD, Casas E, Cundiff LV, Koohmaraie M (2001) The effects of Piedmontese inheritance and myostatin genotype on the palatability of longissimus thoracis, gluteus medius, semimembranosus, and biceps femoris. Journal of Animal Science 79, 3069–3074.
|  CAS | PubMed |
open url image1

Wiener P, Smith JA, Lewis AM, Woolliams JA, Williams JL (2002) Muscle-related traits in cattle: the role of the myostatin gene in the South Devon breed. Genetics, Selection, Evolution 34, 221–232.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1