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RESEARCH ARTICLE
Contents Vol 49(3)

Influence of dicyandiamide on nitrogen transformation and losses in cow-urine-amended soil cores from grazed pasture

Jagrati Singh A B D , S. Saggar A and N. S. Bolan B C
+ Author Affiliations
- Author Affiliations

A Landcare Research, Private Bag 11052, Palmerston North 4442, New Zealand.

B Formerly at Institute of Natural Resources, Massey University, Palmerston North, New Zealand.

C Centre for Environmental Risk Assessment and Remediation, University of South Australia, North Terrace, Adelaide, SA 5000, Australia.

D Corresponding author. Email: Singhj@landcareresearch.co.nz

Animal Production Science 49(3) 253-261 https://doi.org/10.1071/EA08200
Submitted: 17 July 2008  Accepted: 12 November 2008   Published: 2 March 2009

Abstract

In New Zealand, urine deposited by grazing animals represents the largest source of nitrogen (N) losses, as gaseous emissions of ammonia (NH3) and nitrous oxide (N2O), and leaching of nitrate (NO3−).We determined the effect of dicyandiamide (DCD) on gaseous emissions from pasture with increasing rates of urine-N application, mineral N transformations and potential leaching of N using undisturbed soil cores of Manawatu sandy loam at field capacity. The treatments included four levels of urine-N applied at 0 (control), 14.4, 29.0 and 57.0 g N/m2 with and without DCD at 2.5 g/m2. Results showed a significant (P < 0.05) increase in NH3 and N2O-N emissions as urine application was increased. The addition of DCD to corresponding urine treatments reduced N2O emissions by 33, 56 and 80%, respectively. The addition of DCD with urine to the intact soil cores at field capacity moisture content resulted in a significant increase in the soil ammonium-N (NH4+-N) concentration but little change in NH3 emissions. Addition of DCD to urine reduced potential NO3−-N leaching by 60–65% but potential NH4+-N leaching increased by 2–3.5 times. There was no difference in pasture dry matter production with and without DCD treatments.

Additional keyword: nitrification inhibitor.


Acknowledgements

We would like to thank Dr Kevin Tate for his valuable comments and Pete McGregor for editorial suggestions.


References


Amberger A (1989) Research on dicyandiamide as a nitrification inhibitor and future outlook. Communications in Soil Science and Plant Analysis 20, 1933–1955.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Ambus P, Petersen SO, Soussana JF (2007) Short-term carbon and nitrogen cycling in urine patches assessed by combined carbon-13 and nitrogen-15 labelling. Agriculture Ecosystems & Environment 121, 84–92.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Anger M, Hoffmann C, Kuhbauch W (2003) Nitrous oxide emissions from artificial urine patches applied to different N-fertilized swards and estimated annual N2O emissions for differently fertilized pastures in an upland location in Germany. Soil Use and Management 19, 104–111.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Aulakh MS, Rennie DA, Paul EA (1984) Acetylene and N-serve effects upon N2O emissions from NH4 + and NO3 −-treated soils under aerobic and anaerobic conditions. Soil Biology & Biochemistry 16, 351–356.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Blakemore LC , Searle PL , Daly BK (1987) Methods for chemical analysis of soils. New Zealand Soil Bureau Science Report No. 80. Lower Hutt.

Bolan NS, Saggar S, Luo JF, Bhandral R, Singh J (2004) Gaseous emissions of nitrogen from grazed pastures: processes, measurements and modelling, environmental implications and mitigation. Advances in Agronomy 84, 37–120.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Bouwman AF (1990) Exchange of greenhouse gases between terrestrial ecosystems and the atmosphere. In ‘Soils and the greenhouse effect’. (Ed. AF Bouwman) pp. 61–127. (John Wiley and Sons Ltd: Chichester)

Bremner JM , Tabatabai MA (1971) Use of automated combustion techniques for total carbon, total nitrogen, and total sulphur analysis of soils. In ‘Instrumental methods for analysis of soils and plant tissue’. (Ed. LM Walsh) pp. 1–14. (Soil Science Society of America: Madison, WI)

Bremner JM, Blackmer AM, Waring SA (1980) Formation of nitrous-oxide and dinitrogen by chemical decomposition of hydroxylamine in soils. Soil Biology & Biochemistry 12, 263–269.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Bronson KF, Mosier AR, Bishnoi SR (1992) Nitrous-oxide emissions in irrigated corn as affected by nitrification inhibitors. Soil Science Society of America Journal 56, 161–165.
|  CAS |
open url image1

Bronson KF, Sparling GP, Fillery IRP (1999) Short-term N dynamics following application of 15N-labeled urine to a sandy soil in summer. Soil Biology & Biochemistry 31, 1049–1057.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Clark H , Pinares C , de Klien CAM (2005) Methane and nitrous oxide emissions from grazed grasslands. In ‘Grassland: a global resource’. (Ed. DA McGilloway) pp. 279–294. (Wageningen Academic Publishers: Wageningen, The Netherlands)

Clough TJ, Ledgard SF, Sprosen MS, Kear MJ (1998) Fate of 15N labelled urine on four soil types. Plant and Soil 199, 195–203.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Clough TJ, Sherlock RR, Mautner MN, Milligan DB, Wilson PF, Freeman CG, McEwan MJ (2003) Emission of nitrogen oxides and ammonia from varying rates of applied synthetic urine and correlations with soil chemistry. Australian Journal of Soil Research 41, 421–438.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Cookson WR, Cornforth IS (2002) Dicyandiamide slows nitrification in dairy cattle urine patches: effects on soil solution composition, soil pH and pasture yield. Soil Biology & Biochemistry 34, 1461–1465.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Davies DM, Williams PJ (1995) The effect of the nitrification inhibitor dicyandiamide on nitrate leaching and ammonia volatilization – a UK nitrate sensitive areas perspective. Journal of Environmental Management 45, 263–272.
| Crossref | GoogleScholarGoogle Scholar | open url image1

de Klein CAM, Barton L, Sherlock RR, Zheng L, Littlejohn RP (2003) Estimating a nitrous oxide emission factor for animal urine from some New Zealand pastoral soils. Australian Journal of Soil Research 41, 381–399.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Di HJ, Cameron KC (2002a) Nitrate leaching in temperate agroecosystems: sources, factors and mitigating strategies. Nutrient Cycling in Agroecosystems 64, 237–256.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Di HJ, Cameron KC (2002b) The use of a nitrification inhibitor, dicyandiamide (DCD), to decrease nitrate leaching and nitrous oxide emissions in a simulated grazed and irrigated grassland. Soil Use and Management 18, 395–403.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Di HJ, Cameron KC (2003) Mitigation of nitrous oxide emissions in spray-irrigated grazed grassland by treating the soil with dicyandiamide, a nitrification inhibitor. Soil Use and Management 19, 284–290.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Di HJ, Cameron KC (2004) Treating grazed pasture soil with a nitrification inhibitor, eco-n (TM), to decrease nitrate leaching in a deep sandy soil under spray irrigation – a lysimeter study. New Zealand Journal of Agricultural Research 47, 351–361.
|  CAS |
open url image1

Di HJ, Cameron KC (2005) Reducing environmental impacts of agriculture by using a fine particle suspension nitrification inhibitor to decrease nitrate leaching from grazed pastures. Agriculture Ecosystems & Environment 109, 202–212.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Di HJ, Cameron KC (2007a) Comparison of the effectiveness of a nitrification inhibitor, dicyandiamide, in reducing nitrous oxide emissions in four different soils under different climatic and management conditions. Soil Use and Management 23, 1–9.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Di HJ, Cameron KC (2007b) Nitrate leaching losses and pasture yields as affected by different rates of animal urine nitrogen returns and application of a nitrification inhibitor – a lysimeter study. Nutrient Cycling in Agroecosystems 79, 281–290.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Doak BW (1952) Some chemical changes in the nitrogenous constituents of urine when voided on pasture. Journal of Agriculture Science, Cambridge 42, 162–171.
|  CAS |
open url image1

Ebina J, Tsutsui T, Shirai T (1983) Simultaneous determination of total nitrogen and total phosphorus in water using peroxodisulfate oxidation. Water Research 17, 1721–1726.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Firestone MK , Davidson EA (1989) Microbiological basis of NO and N2O production and consumption in soil. In ‘Exchange of trace gasses between terrestrial ecosystems and the atmosphere’. (Eds MO Andreae, DS Schimel) pp. 7–21. (John Wiley: New York)

Gioacchini P, Nastri A, Marzadori C, Giovannini C, Antisari LV, Gessa C (2002) Influence of urease and nitrification inhibitors on N losses from soils fertilized with urea. Biology and Fertility of Soils 36, 129–135.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Hedley CB, Saggar S, Tate KR (2006) Procedure for fast simultaneous analysis of the greenhouse gases: methane, carbon dioxide, and nitrous oxide in air samples. Communications in Soil Science and Plant Analysis 37, 1501–1510.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Hewitt AE (1998) ‘New Zealand soil classification.’ 2nd edn. (Manaaki Whenua Press: Lincoln, New Zealand)

Holmes W (1968) The use of nitrogen in the management of pasture for cattle. Herbage Abstracts 38, 265–277. open url image1

Hoogendoorn CJ, de Klein CAM, Rutherford AJ, Letica S, Devantier BP (2008) The effect of increasing rates of nitrogen fertiliser and a nitrification inhibitor on nitrous oxide emissions from urine patches on sheep grazed hill country pasture. Australian Journal of Experimental Agriculture 48, 147–151.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Jarvis SC, Hatch DJ, Pain BF, Klarenbeek JV (1994) Denitrification and the evolution of nitrous oxide after the application of cattle slurry to a peat soil. Plant and Soil 166, 231–241.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Kelliher FM, Sedcole JR, Minchin RF, Wan Y, Condron LM, Clough TJ, Bol R (2005) Soil microbial respiration responses to repeated urea applications in three grasslands. Australian Journal of Soil Research 43, 905–913.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Kelly KB, Phillips FA, Baigent R (2008) Impact of dicyandiamide application on nitrous oxide emissions from urine patches in northern Victoria. Australian Journal of Experimental Agriculture 48, 156–159.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Linn DM, Doran JW (1984) Effect of water filled pore space on carbon dioxide and nitrous oxide production in tilled and nontilled soils. Soil Science Society of America Journal 48, 1267–1272.
|  CAS |
open url image1

Lockyer DR, Whitehead DC (1990) Volatilization of ammonia from cattle urine applied to grassland. Soil Biology & Biochemistry 22, 1137–1142.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

MAF (2003) A short-term financial and physical forecast reflecting farmer, farm consultant and industry perceptions of farming trends and issues, production and financial figures. Dairy monitoring report. Ministry of Agriculture and Forestry, Wellington, New Zealand. p. 58.

McKenzie HA, Wallace HS (1954) The Kjeldahl determination of nitrogen: a critical study of digestion conditions-temperature, catalyst and oxidising agent. Australian Journal of Chemistry 7, 55–70.
|  CAS |
open url image1

Merino P, Estavillo JM, Graciolli LA, Pinto M, Lacuesta M, Munoz-Rueda A, Gonzalez-Murua C (2002) Mitigation of N2O emissions from grassland by nitrification inhibitor and Actilith F2 applied with fertilizer and cattle slurry. Soil Use and Management 18, 135–141. open url image1

MfE (2007) New Zealand’s Greenhouse Gas Inventory 1990–2005. The National Inventory Report and Common Reporting Format, July 2007. Ministry for the Environment, Wellington, New Zealand. p. 158.

Monaghan RM, Barraclough D (1992) Some chemical and physical factors affecting the rate and dynamics of nitrification in urine-affected soil. Plant and Soil 143, 11–18.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Oenema O, Velthof GL, Yamulki S, Jarvis SC (1997) Nitrous oxide emissions from grazed grassland. Soil Use and Management 13, 288–295.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Puttanna K, Gowda NMN, Rao EVSP (2001) Regulation of nitrification by benzotriazole, o-Nitrophenol, m-Nitroaniline and dicyandiamide and pattern of NH3 emissions from citronella field fertilized with urea. Water, Air, and Soil Pollution 131, 11–17.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Quin BF (1977) The fate of sheep urine-nitrogen on surface irrigated pasture in Canterbury. In ‘New Zealand Soil News.’ Suppl. 25, No. 4.

Rao EVSP, Puttanna K (1987) Nitrification and ammonia volatilization losses from urea and dicyandiamide-treated urea in a sandy loam soil. Plant and Soil 97, 201–206.
|  CAS | Crossref |
open url image1

Richards IR, Wolton KM (1975) A note on urine scorch caused by grazing animals. Journal of the British Grassland Society 30, 187–188. open url image1

Rodgers GA (1983) Effect of dicyandiamide on ammonia volatilisation from urea in soil. Fertilizer Research 4, 361–367.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Saggar S, Andrew RM, Tate KR, Hedley CB, Rodda NJ, Townsend JA (2004a) Modelling nitrous oxide emissions from dairy-grazed pastures. Nutrient Cycling in Agroecosystems 68, 243–255.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Saggar S, Bolan NS, Bhandral R, Hedley CB, Luo J (2004b) A review of emissions of methane, ammonia and nitrous oxide from animal excreta deposition and farm effluent application in grazed pastures. New Zealand Journal of Agricultural Research 47, 513–544.
|  CAS |
open url image1

Sheppard SC , Bittman S , Tait J (2005) Air quality – ammonia. In ‘Environmental sustainability of Canadian agriculture: Agri-Environmental Indicator Report Series – Report #2’. (Eds A Lefebvre, W Eilers, B Chunn) pp. 149–152. (Agriculture and Agri-Food Canada: Ottawa, Ontario)

Singh J , Bolan N , Saggar S (2003) A method for simultaneous measurement of ammonia volatilization and nitrous oxide emissions from intact soil cores. In ‘Tools for nutrient and pollutant management: applications to agriculture and environmental quality, Palmerston North’. (Eds LD Currie, JA Hanly) pp. 224–231. (Fertilizer and Lime Research Centre, Massey University: Palmerston North, New Zealand)

Smith LC, Monaghan RM, Ledgard SF, Catto WD (2005) The effectiveness of different nitrification inhibitor formulations in limiting accumulation in a Southland pastoral soil. New Zealand Journal of Agricultural Research 48, 517–529.
|  CAS |
open url image1

Smith LC, de Klein CAM, Monaghan RM, Catto WD (2008) The effectiveness of dicyandiamide in reducing nitrous oxide emissions from a cattle-grazed, winter forage crop in Southland, New Zealand. Australian Journal of Experimental Agriculture 48, 160–164.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Stevens RJ, Laughlin RJ (1998) Measurement of nitrous oxide and di-nitrogen emissions from agricultural soils. Nutrient Cycling in Agroecosystems 52, 131–139.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Tietema A, Wessel WW (1992) Gross nitrogen transformations in the organic layer of acid forest ecosystems subjected to increased atmospheric nitrogen input. Soil Biology & Biochemistry 24, 943–950.
| Crossref | GoogleScholarGoogle Scholar | open url image1

van Groenigen JW, Kuikman PJ, De Groot WJM, Velthof GL (2005) Nitrous oxide emission from urine-treated soil as influenced by urine composition and soil physical conditions. Soil Biology & Biochemistry 37, 463–473.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Wadman WP, Neeteson JJ, Wijnen GJ (1993) Field experiments with slurry and dicyandiamide – response of potatoes and effects on soil mineral nitrogen. Netherlands Journal of Agricultural Science 41, 95–109.
|  CAS |
open url image1

Whitehead DC (1995) ‘Grassland nitrogen.’ (CAB International: Wallingford, UK)

Whitehead DC, Bristow AW (1990) Transformations of nitrogen following the application of 15N-labelled cattle urine to an established grass sward. Journal of Applied Ecology 27, 667–678.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Williams DL, Ineson P, Coward PA (1999) Temporal variations in nitrous oxide fluxes from urine-affected grassland. Soil Biology & Biochemistry 31, 779–788.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Williamson JC, Taylor MD, Torrens RS, Vojvodic-Vukovic M (1998) Reducing nitrogen leaching from dairy farm effluent-irrigated pasture using dicyandiamide: a lysimeter study. Agriculture Ecosystems & Environment 69, 81–88.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Wulf S, Maeting M, Bergmann S, Clemens J (2001) Simultaneous measurement of NH3, N2O and CH4 to assess efficiency of trace gas emission abatement after slurry application. Phyton (Austria) 41, 131–142.
|  CAS |
open url image1