Effect of surface application of benzylaminopurine before and during water deficit on herbage production and quality
J. M. Lee A E , K. Elborough B C , D. Whittaker B , D. J. Donaghy D and J. R. Roche DA DairyNZ Limited, Private Bag 3221, Hamilton 3240, New Zealand.
B ViaLactia BioSciences, PO Box 109185, Auckland 1061, New Zealand.
C Present address: HortResearch, 120 Mount Albert Road, Auckland 1025, New Zealand.
D University of Tasmania, PO Box 3253, Burnie, Tas. 7320, Australia.
E Corresponding author. Email: julia.lee@dairynz.co.nz
Australian Journal of Experimental Agriculture 48(9) 1232-1236 https://doi.org/10.1071/EA07075
Submitted: 15 March 2007 Accepted: 4 July 2008 Published: 7 August 2008
Abstract
Insufficient water availability is one of the most serious environmental stresses for a plant and can be a major limitation to herbage production in many regions. Plant hormones, such as cytokinins, have been reported to reduce the detrimental effects of low water availability in many different plant species. However, there is little research available concerning the effect on pasture grasses. The aim of the present study was to quantify the effect of surface applications of 6-benzylaminopurine (BAP) before and during water deficit on herbage growth and quality of perennial ryegrass–white clover pastures. Pastures fertilised with BAP were compared with unfertilised pastures and pastures receiving nitrogen. Exogenous BAP application did not significantly affect herbage production or quality relative to unfertilised pastures. Nitrogen fertiliser increased total herbage production, but had little effect on herbage quality compared with BAP. The failure of surface applications of exogenous BAP to improve herbage production or quality suggests that it is not an appropriate method to enhance plant tolerance to water deficit at the concentration applied in this study.
Acknowledgements
The authors would like to acknowledge the statistical expertise of Barbara Dow, the help afforded by Scott Farm staff and the technical expertise of Glenise Ferguson, Jacqueline Kimber and Talia and Jordan van der Wel. This project was funded by ViaLactia BioSciences.
Adedipe NO,
Hunt LA, Fletcher RA
(1971) Effects of benzyladenine on photosynthesis, growth and senescence of the bean plant. Physiologia Plantarum 25, 151–153.
| Crossref | GoogleScholarGoogle Scholar |
CAS |
Agarwal RK, Gupta SC
(1995) Plant growth substances as osmoregulants under salt stress in callus cultures of cowpea. Indian Journal of Plant Physiology 38, 325–327.
|
CAS |
Badenoch-Jones J,
Parker CW,
Letham DS, Singh S
(1996) Effect of cytokinins supplied via the xylem at multiples of endogenous concentrations on transpiration and senescence in derooted seedlings of oat and wheat. Plant, Cell & Environment 19, 504–516.
| Crossref | GoogleScholarGoogle Scholar |
CAS |
Chernyad’ev II
(2000) Ontogenetic changes in the photosynthetic apparatus and effects of cytokinins Applied Biochemistry and Microbiology 36, 527–528.
| Crossref | GoogleScholarGoogle Scholar |
Chernyad’ev II
(2005) Effect of water stress on the photosynthetic apparatus of plants and the protective role of cytokinins: a review. Applied Biochemistry and Microbiology 41, 115–128.
| Crossref | GoogleScholarGoogle Scholar |
CAS |
Chernyad’ev II, Monakhova OF
(2003) Effects of cytokinin preparations on the pools of pigments and proteins of wheat cultivars differing in their tolerance to water stress. Applied Biochemistry and Microbiology 39, 524–531.
| Crossref | GoogleScholarGoogle Scholar |
CAS |
Corson DC,
Waghorn GC,
Ulyatt MJ, Lee J
(1999) NIRS: forage analysis and livestock feeding. Proceedings of the New Zealand Grassland Association 61, 127–132.
David MM,
Coelho D,
Barrote I, Correia MJ
(1998) Leaf age effects on photosynthetic activity and sugar accumulation in droughted and rewatered Lupinus albus plants. Australian Journal of Plant Physiology 25, 299–306.
|
CAS |
Diettrich B,
Mertinat H, Luckner M
(1992) Reduction of water loss during ex vitro acclimatization of micropropagated Digitalis lanata clone plants. Biochemie und Physiologie der Pflanzen 188, 23–31.
Fulkerson WJ, Donaghy DJ
(2001) Plant-soluble carbohydrate reserves and senescence – key criteria for developing an effective grazing management system for ryegrass-based pastures: a review. Australian Journal of Experimental Agriculture 41, 261–275.
| Crossref | GoogleScholarGoogle Scholar |
CAS |
Karsten HD, MacAdam JW
(2001) Effect of drought on growth, carbohydrates, and soil water use by perennial ryegrass, tall fescue, and white clover. Crop Science 41, 156–166.
|
CAS |
Kemp CD
(1960) Methods of estimating the leaf area of grasses from linear measurements. Annals of Botany 24, 491–499.
Kumar B,
Pandey DM,
Goswami CL, Jain S
(2001) Effect of growth regulators on photosynthesis, transpiration and related parameters in water stressed cotton. Biologia Plantarum 44, 475–478.
| Crossref | GoogleScholarGoogle Scholar |
CAS |
Lara MEB,
Garcia MG,
Fatima T,
Ehneß R,
Lee TK,
Proels R,
Tanner W, Roitsch T
(2004) Extracellular invertase is an essential component of cytokinin-mediated delay of senescence. The Plant Cell 16, 1276–1287.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Leopold AC, Kawase M
(1964) Benzyladenine effects on bean leaf growth and senescence. American Journal of Botany 51, 294–298.
| Crossref | GoogleScholarGoogle Scholar |
CAS |
Letham DS, Palni LMS
(1983) The biosynthesis and metabolism of cytokinins. Annual Review of Plant Physiology 34, 163–197.
| Crossref | GoogleScholarGoogle Scholar |
CAS |
Monakhova OF, Chernyad’ev II
(2004) Effects of cytokinin preparations on the stability of the photosynthetic apparatus of two wheat cultivars experiencing water deficiency. Applied Biochemistry and Microbiology 40, 573–580.
| Crossref | GoogleScholarGoogle Scholar |
CAS |
Niedzwiedz-Siegeń I, Lewak S
(1992) Growth regulators differentially affect photosensitivity induced by low temperature and osmotic stresses in germinating white clover seeds. Plant Growth Regulation 11, 133–137.
| Crossref | GoogleScholarGoogle Scholar |
Pandey DM,
Goswami CL, Kumar B
(2003) Physiological effects of plant hormones in cotton under drought. Biologia Plantarum 47, 535–540.
| Crossref | GoogleScholarGoogle Scholar |
CAS |
Pospíšilová J
(2003) Participation of phytohormones in the stomatal regulation of gas exchange during water stress. Biologia Plantarum 46, 491–506.
| Crossref | GoogleScholarGoogle Scholar |
Pospíšilová J,
Synková H, Rulcová J
(2000) Cytokinins and water stress. Biologia Plantarum 43, 321–328.
| Crossref | GoogleScholarGoogle Scholar |
Privé JP, Janes D
(2003) Evaluation of plant and soil moisture sensors for the detection of drought stress in raspberry. Acta Horticulturae 618, 391–396.
Ron’zhina ES
(2003) Effect of 6-benzylaminopurine on the structure of the photosynthetic apparatus of faba bean (Vicia faba L.). Applied Biochemistry and Microbiology 39, 411–417.
| Crossref | GoogleScholarGoogle Scholar |
CAS |
Rulcová J, Pospíšilová J
(2001) Effect of benzylaminopurine on rehydration of bean plants after water stress. Biologia Plantarum 44, 75–81.
| Crossref | GoogleScholarGoogle Scholar |
Takegami T
(1975) A study on senescence in tobacco leaf disks. I. Inhibition by benzylaminopurine of decrease in protein levels. Plant & Cell Physiology 16, 407–416.
|
CAS |
Thomas JC,
McElwain EF, Bohnert HJ
(1992) Convergent induction of osmotic stress-responses. Plant Physiology 100, 416–423.
|
CAS |
PubMed |
Vogelmann TC,
Bornman CH, Nissen P
(1984) Uptake of benzyladenine in explants of Picea abies and Pinus sylvestris. Physiologia Plantarum 61, 513–517.
| Crossref | GoogleScholarGoogle Scholar |
CAS |
Yadav N,
Gupta V, Yadav VK
(1997) Role of benzyladenine and gibberellic acid in alleviating water-stress effect in gram (Cicer arietinum). Indian Journal of Agricultural Science 67, 381–387.
|
CAS |