Register      Login
Animal Production Science Animal Production Science Society
Food, fibre and pharmaceuticals from animals
Shopping Cart: ( empty )
You are here: Home > Journals > AN > EA06337
RESEARCH ARTICLE
Contents Vol 47(9)

Variation among hexaploid Paspalum dilatatum Poir. regenerants from tissue culture

B. C. Venuto A B G , S. S. Croughan C , W. D. Pitman D , R. W. Jessup E , K. Renganayaki F and B. L. Burson E
+ Author Affiliations
- Author Affiliations

A Louisiana State University Agricultural Center, Agronomy Department, Baton Rouge, LA 70803, USA.

B Present address: USDA, Agricultural Research Service, El Reno, OK 73036, USA.

C Louisiana State University Agricultural Center, Rice Research Station, Crowley, LA 70527, USA.

D Louisiana State University Agricultural Center, Rosepine Research Station, Rosepine, LA 70659, USA.

E United States Department of Agriculture, Agricultural Research Service, Crop Germplasm Research Unit, College Station, TX 77843, USA.

F Texas A&M University, Department of Soil and Crop Sciences, College Station, TX 77843, USA.

G Corresponding author. Email: bvenuto@grl.ars.usda.gov

Australian Journal of Experimental Agriculture 47(9) 1109-1116 https://doi.org/10.1071/EA06337
Submitted: 2 January 2007  Accepted: 22 May 2007   Published: 6 August 2007

Abstract

The common biotype of Paspalum dilatatum Poir. (dallisgrass) is a pentaploid obligate apomict and efforts to improve the grass have not been successful because of its asexual reproduction and irregular meiosis. An apomictic hexaploid biotype, known as Uruguayan dallisgrass, is a new source of genetic variation that may be useful in improving dallisgrass. As with common dallisgrass, improvement of this biotype via conventional breeding methods is difficult because of its apomictic reproduction. However, the use of tissue culture to produce somaclonal variation in the Uruguayan biotype has not been reported, and may offer a means for improving the species. The objectives of this research were to: (i) regenerate plants of Uruguayan dallisgrass through tissue culture, (ii) screen the regenerants for useful agronomic variation and evaluate their forage potential and nutritive value, and (iii) determine the genetic relatedness of the regenerants and their explant sources. In total, 178 plants, selected from 2372 regenerants in preliminary screening, were evaluated for forage nutritive value. Thirty-seven of these were planted into replicated field plot trials at two locations. None of these regenerants were superior to the Uruguayan biotype for forage nutritive value. However, two regenerants, 3440 and 3441, produced more forage than either the Uruguayan or common biotypes in evaluation tests for 3 years at one of the two locations. Data from AFLP analyses indicate genetic variation between two of the Uruguayan accessions and these two regenerants. This variation could account for the differences in forage yield between 3440 and 3441 and the Uruguayan accessions.


References


Akashi R, Adachi T (1992) Somatic embryogenesis and plant regeneration from immature inflorescences of apomictic dallisgrass (Paspalum dilatatum Poir.) Plant Science 82, 213–218.
| Crossref | GoogleScholarGoogle Scholar | open url image1

AOAC (1990) ‘Official methods of analysis.’ 15th edn. (Association of Official and Analytical Chemists: Washington, DC)

Bashaw EC, Forbes I (1958) Chromosome numbers and microsporogenesis in dallisgrass Paspalum dilatatum Poir. Agronomy Journal 50, 441–445. open url image1

Bashaw EC, Holt EC (1958) Megasporogenesis, embryo sac development and embryogenesis in dallisgrass, Paspalum dilatatum Poir. Agronomy Journal 50, 753–756. open url image1

Brown HB, Ranck EM (1915) Forage poisoning due to Claviceps paspali on Paspalum. Mississippi Agricultural Experiment Station, Agricultural College, Technical Bulletin No. 6.

Bryan WW (1970) Changes in botanical composition in some sub-tropical zone pastures. In ‘Proceedings of the XI international grassland congress, Surfers Paradise, Queensland’. (Ed. MJT Norman) pp. 636–639. (University of Queensland Press: St. Lucia, Qld)

Burson BL (1978) Genome relations between Paspalum conspersum and two diploid Paspalum species. Canadian Journal of Genetics and Cytology 20, 365–372. open url image1

Burson BL (1983) Phylogenetic investigations of Paspalum dilatatum and related species. In ‘Proceedings of the XIV international grassland congress, Lexington, Kentucky’. (Eds JA Smith, VW Hays) pp. 170–173. (Westview Press: Boulder, CO)

Burson BL (1991) Genome relationships between tetraploid and hexaploid biotypes of dallisgrass, Paspalum dilatatum. Botanical Gazette (Chicago, Ill.) [Chicago] 152, 219–223.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Burson BL, Tischler CR (1993) Regeneration and somaclonal variation in apomictic Paspalum dilatatum Poir. Euphytica 67, 71–78.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Burson BL, Watson VH (1995) Bahiagrass, dallisgrass and other Paspalum species. In ‘Forages, Vol I: an introduction to grassland agriculture’. (Eds RF Barnes, DA Miller, CJ Nelson) pp. 431–440. (Iowa State University Press: Ames, IA)

Burson BL, Voigt PW, Evers GW (1991) Cytology, reproductive behavior, and forage potential of hexaploid dallisgrass biotypes. Crop Science 31, 636–641. open url image1

Callow MN, Fulkerson WJ, Donaghy DJ, Morris RJ, Sweeney G, Upjohn B (2005) Responses of perennial ryegrass (Lolium perenne) to renovation in Australian dairy pastures. Australian Journal of Experimental Agriculture 45, 1559–1565.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Campbell LRV (1999) Paspalum dilatatum and Axonopus affinis in Australia. In ‘Forage seed production Vol. 2: tropical and subtropical species’. (Eds DS Loch, JE Ferguson) pp. 325–333. (CABI Publishing: Wallingford, UK)

Casa AM, Mitchell S, Lopes CR, Valls JFM (2002) RAPD analyses reveals genetic variability among sexual and apomitic Paspalum dilatatum Poiret biotypes. The Journal of Heredity 93, 300–302.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Chase A (1929) North American species of Paspalum. Contributions to the U.S. National Herbarium 28(1), 1–310.

Davies LJ, Cohen D (1992) Phenotypic variation in somaclones of Paspalum dilatatum and their seedling offspring. Canadian Journal of Plant Science 72, 773–784. open url image1

Davies LJ, Cohen D, Mullins KV (1986) Somaclonal variation in the apomictic pasture grass Paspalum dilatatum. New Zealand Agronomy Society, Special Publication No. 5.

Evers GW, Burson BL (2004) Dallisgrass and other Paspalum species. In ‘Warm-season (C4) grasses, Agronomy Monograph No. 45’. (Eds LE Moser, BL Burson, LE Sollenberger) pp. 681–713. (American Society of Agronomy, Crop Science Society of America, Soil Science Society of America, Inc.: Madison, WI)

Goering HK, Van Soest PJ (1970) ‘Forage fiber analysis (apparatus, reagents, procedures, and some applications). Agriculture Handbook 379.’ (U.S. Government Printing Office, Washington, DC)

Holt EC (1956) Dallisgrass. Texas Agricultural Experiment Station, Bulletin 829.

Lawson AR, Kelly KB (2007) Responses to renovation of an irrigated perennial pasture in northern Victoria. 1. Pasture consumption and plant tiller densities. Australian Journal of Experimental Agriculture 47, 149–158.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Matthes M, Singh R, Cheah S-C, Karp A (2001) Variation in oil palm (Elaeis guineensis Jacq.) tissue culture-derived regenerants revealed by AFLPs with methylation-sensitive enzymes. Theoretical and Applied Genetics 102, 971–979.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Mian MAR, Zwonitzer JC, Chen Y, Saha MC, Hopkins AA (2005) AFLP diversity within and among hardinggrass populations. Crop Science 45, 2591–2597.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Miz RB, Souza-Chies TT (2006) Genetic relationships and variation among biotypes of dallisgrass (Paspalum dilatatum Poir.) and related species using random amplified polymorphic DNA markers. Genetic Resources and Crop Evolution 53, 541–552.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15, 473–497.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Pejic I, Ajmone-Marsan P, Morgante M, Kozumplick V, Castiglioni P, Taramino G, Motto M (1998) Comparative analysis of genetic similarity among maize inbred lines detected by RFLPs, RAPDs, SSRs, and AFLPs. Theoretical and Applied Genetics 97, 1248–1255.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Pitman WD, Croughan SS, Nash JL, Venuto BC (2005) Somaclonal variation in seed germination of dallisgrass biotypes. Plant Genetic Resources 3, 414–420. open url image1

Powell W, Morgante M, Andre C, Hanafey M, Vogel J, Tingey S, Rafalsky A (1996) The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis. Molecular Breeding 2, 225–238.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Renganayaki K, Read JC, Fritz AK (2001) Genetic diversity among Texas bluegrass genotypes (Poa arachnifera Torr.) revealed by AFLP and RAPD markers. Theoretical and Applied Genetics 102, 1037–1045.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Rohlf FJ (1997) ‘NTSYS-pc numerical taxonomy and multi-variate analysis system, Version 2.1.’ (Exeter Software: Setauket, NY)

Russell JR, Fuller JD, Macaulay M, Hatz BG, Jahoor A, Powell W, Waugh R (1997) Direct comparison of levels of genetic variation among barley accessions detected by RFLPs, AFLPs, SSRs and RAPDs. Theoretical and Applied Genetics 95, 714–722.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Shaw NH, Elich TW, Haydock KP, Waite RB (1965) A comparison of seventeen introductions of Paspalum species and naturalized P. dilatatum under cutting at Samford, south-eastern Queensland. Australian Journal of Experimental Agriculture and Animal Husbandry 5, 423–432.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Shenk JS, Westerhaus MO (1991) Population structuring of near-infrared spectra and modified partial least squares regression. Crop Science 31, 1548–1555. open url image1

Tischler CR, Burson BL (1999) Seed dormancy and germination of dallisgrass, Paspalum dilatatum, stored under differing conditions. Seed Science and Technology 27, 263–271. open url image1

Tischler CR, Burson BL, Jordan WR (1993) Physiological variation in tissue culture regenerated apomictic Paspalum dilatatum. Journal of Plant Physiology 141, 482–486. open url image1

Ubi EB, Kolliker R, Fujimori M, Komatsu T (2003) Genetic diversity in diploid cultivars of rhodesgrass determined on the basis of amplified fragment length polymorphism markers. Crop Science 43, 1516–1522. open url image1

Van Soest PJ, Robertson JB (1980) Systems of analysis for evaluating fibrous feeds. In ‘Proceedings of an international workshop on standardization of analytical methods for feeds, Ottawa, Canada’. (Eds WJ Pigden, CC Balch, M Graham) pp. 49–60. (Unipub: New York)

Venuto BC, Burson BL, Hussey MA, Redfearn DD, Wyatt WE, Brown LP (2003) Forage yield, nutritive value, and grazing tolerance of dallisgrass biotypes. Crop Science 43, 295–301. open url image1

Vos P, Hogers R, Bleeker M, Reijans M, Lee T , et al. (1995) A new technique for DNA fingerprinting. Nucleic Acids Research 23, 4407–4414.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Wales WJ, Dellow DW, Doyle PT (2000) Protein supplementation of cows grazing limited amounts of paspalum (Paspalum dilatatum Poir.)-dominant irrigated pastures in mid lactation. Australian Journal of Experimental Agriculture 40, 923–929.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Walker GP, Stockdale CR, Wales WJ, Doyle PT, Dellow DW (2001) Effect of level of grain supplementation on milk production responses of dairy cows in mid-late lactation when grazing irrigated pastures high in paspalum (Paspalum dilatatum Poir.). Australian Journal of Experimental Agriculture 41, 1–11.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Whiteman PC (1980) ‘Tropical pasture science.’ (Oxford University Press: Oxford, UK)

Williams CE, Ronald PC (1994) PCR template-DNA isolated quickly from monocot and dicot leaves without tissue homogenization. Nucleic Acids Research 22, 1917–1918.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1









1 Mention of a trademark or proprietary product does not constitute a guarantee or warranty of the product by the US Department of Agriculture and does not imply its approval to the exclusion of other products that may also be available.