Tropical alfalfa (Medicago sativa cv. Kacang Ratu BW) supplementation for reducing cholesterol and improving quality of carcass and meat of hybrid duck
Bambang Suwignyo
A * , Eprilia Aristia Rini B , Ukad Wahyudi A , Edi Suryanto A ,
+ Author Affiliations
- Author Affiliations
A Faculty of Animal Science, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia.
B Ministry of Agriculture Republic of Indonesia, South Jakarta, Indonesia.
Animal Production Science 63(5) 471-479 https://doi.org/10.1071/AN22018
Submitted: 25 January 2022 Accepted: 25 October 2022 Published: 18 November 2022
© 2023 The Author(s) (or their employer(s)). Published by CSIRO Publishing
Abstract
Context: Duck meat is a good source of protein (21.4%) but it is high in cholesterol, with a standard range of 125–200 mg/dL. Alfalfa is a legume that contains saponins, which have been shown to reduce the cholesterol content of meat.
Aim: We aimed to determine the effect of alfalfa on the weight of the internal organs and carcass, the physicochemical quality of meat, and the cholesterol of hybrid ducks in Indonesia.
Methods: Ninety 1-day-old Indonesian hybrid ducks were fed basal rations supplemented with 0% (control), 3%, or 6% fresh alfalfa (n = 5 per group), calculated on a dry matter basis. Five replicates were made for each dietary treatment. Dietary treatments were administered to the ducks from 8 to 35 days of age. The parameters measured were the internal organ weight, carcass quality (live weight, carcass weight, carcass percentage, and abdominal fat), physicochemical quality of meat (water content, crude protein, crude fat, pH, water holding capacity [WHC], cooking loss, and tenderness), and the cholesterol content of the liver, breast meat, thigh meat, and blood (including the high-density lipoprotein/HDL and low-density lipoprotein/LDL). Analysis of variance and Duncan’s multiple range tests determined the significance of results.
Key results: Supplementation of rations with up to 6% of alfalfa did not significantly affect the weight of the internal organs, but reduced the cholesterol content in the liver, meat, and blood. In addition, the physicochemical parameter of alfalfa supplementation in the rations, up to a level of 6%, had no effect on carcass quality, moisture content, crude protein or pH of hybrid duck meat. Moreover, supplementation with 6% of alfalfa significantly reduced the LDL levels while increasing the HDL and affecting crude fat, WHC, cooking loss, and tenderness of hybrid ducks.
Conclusions: Supplementation with up to 6% of alfalfa did not affect the weight of key internal digestive organs, but reduced the cholesterol content, and increased the HDL levels of blood. Alfalfa supplementation affected the physicochemical quality of hybrid duck meat. However, the optimum level (production parameters observation) of alfalfa supplementation in the diet was at 3%.
Implications: Hybrid duck meat exhibits lower cholesterol, higher HDL, and lower LDL when the diet is supplemented with alfalfa, whereas other parameters were largely unchanged.
Keywords: carcass, cholesterol, hybrid duck, internal organs, legume, meat quality, physicochemical, saponins.
References
Akoso BT (1993) ‘Manual kesehatan unggas.’ (Kanisius: Yogyakarta)Amrullah IK (2003) ‘Nutrisi Ayam Broiler.’ (Lembaga Satu Gunungbudi: Bogor)
AOAC (1990) ‘Official methods of analysis.’ (Association of Official Analytical Chemist)
Arslan C, Çitil M, Saatci M (2003) Effects of l-carnitine administration on growth performance, carcass traits, blood serum parameters and abdominal fatty acid composition of ducks. Archives of Animal Nutrition 57, 381–388.
| Effects of l-carnitine administration on growth performance, carcass traits, blood serum parameters and abdominal fatty acid composition of ducks.Crossref | GoogleScholarGoogle Scholar |
Astuti M (2007) ‘Pengantar ilmu statistik untuk peternakan dan kesehatan hewan.’ (Binasti: Bogor)
Badan Standardisasi Nasional (2009) ‘Mutu karkas dan daging ayam.’ (Badan Standardisasi Nasional: SNI)
Basmacioglu H, Ergü M (2005) Research on the factors affecting cholesterol content and some other characteristics of eggs in laying hens. Turkish Journal of Veterinary & Animal Sciences 29, 157–164.
Bora KS, Sharma A (2011) Phytochemical and pharmacological potential of Medicago sativa: a review. Pharmaceutical Biology 49, 211–220.
| Phytochemical and pharmacological potential of Medicago sativa: a review.Crossref | GoogleScholarGoogle Scholar |
Borin K, Lindberg JE, Ogle RB (2006) Digestibility and digestive organ development in indigenous and improved chickens and ducks fed diets with increasing inclusion levels of cassava leaf meal. Journal of Animal Physiology and Animal Nutrition 90, 230–237.
| Digestibility and digestive organ development in indigenous and improved chickens and ducks fed diets with increasing inclusion levels of cassava leaf meal.Crossref | GoogleScholarGoogle Scholar |
Buddington RK, Buddington KK, Sunvold GD (1999) Influence of fermentable fiber on small intestinal dimensions and transport of glucose and proline in dogs. American Journal of Veterinary Research 60, 354–358.
Cunniff Association of Official Analytical Chemists (1995) ‘Official methods of analysis of AOAC international.’ (Association of Official Analytical Chemists: Washington, DC)
Dewanti R, Irham M, dan Sudiyono (2013) Pengaruh penggunaan enceng gondok (Eichornia crassipes) terfermentasi dalam ransum terhadap persentase karkas, non-karkas, dan lemak abdominal itik lokal jantan umur delapan minggu. Buletin Peternakan 37, 19–25.
| Pengaruh penggunaan enceng gondok (Eichornia crassipes) terfermentasi dalam ransum terhadap persentase karkas, non-karkas, dan lemak abdominal itik lokal jantan umur delapan minggu.Crossref | GoogleScholarGoogle Scholar |
Fuller R (1997) ‘Probiotics 2: applications and practical aspects.’ (Springer Science & Business Media)
Hardiningsih R, Nurhidayat N (2006) The effect of consuming the hypercholesterolemia rationed food to the body weight of white rats wistar with administration of lactic acid bacteria. Biodiversitas Journal of Biological Diversity 7, 127–130.
| The effect of consuming the hypercholesterolemia rationed food to the body weight of white rats wistar with administration of lactic acid bacteria.Crossref | GoogleScholarGoogle Scholar |
Has H, Napirah A, Indi A (2014) Efek peningkatan serat kasar dengan penggunaan daun murbei dalam ransum broiler terhadap persentase bobot saluran pencernaan. Jurnal Ilmu dan Teknologi Peternakan Tropis 1, 63–69.
| Efek peningkatan serat kasar dengan penggunaan daun murbei dalam ransum broiler terhadap persentase bobot saluran pencernaan.Crossref | GoogleScholarGoogle Scholar |
Hatta U (2005) Performan hati dan ginjal ayam broiler yang diberi ransum menggunakan ubi kayu fermentasi dengan penambahan lysine. Jurnal Agroland 16, 85–90.
Hidayat C (2015) Penurunan deposit lemak abdominal pada ayam pedaging melalui manajemen pakan. Wartazoa 25, 125–134.
| Penurunan deposit lemak abdominal pada ayam pedaging melalui manajemen pakan.Crossref | GoogleScholarGoogle Scholar |
Hidayati NN, Yuniwarti EYW, Isdadiyanto S (2016) Perbandingan kualitas daging itik magelang, itik pengging dan itik tegal. Bioma: Berkala Ilmiah Biologi 18, 56–63.
| Perbandingan kualitas daging itik magelang, itik pengging dan itik tegal.Crossref | GoogleScholarGoogle Scholar |
Incharoen T (2013) Histological adaptations of the gastrointestinal tract of broilers fed diets containing insoluble fiber from rice hull meal. American Journal of Animal and Veterinary Sciences 8, 79–88.
| Histological adaptations of the gastrointestinal tract of broilers fed diets containing insoluble fiber from rice hull meal.Crossref | GoogleScholarGoogle Scholar |
Juniarti N, Ngitung R, Hiola SF (2019) Pengaruh pemberian tepung rumput laut pada ransum ayam broiler terhadap kadar lemak dan kolesterol. Bionature 20, 64–78.
| Pengaruh pemberian tepung rumput laut pada ransum ayam broiler terhadap kadar lemak dan kolesterol.Crossref | GoogleScholarGoogle Scholar |
Kleiner IS, Dotti LB (1962) Laboratory instructions in biochemistry. Academic Medicine 37, 1147
Kokoszynski D, Kotowicz M, Brudnicki A, Bernacki Z, Wasilewski PD, Wasilewski R (2017) Carcass composition and quality of meat from Pekin ducks finished on diets with varying levels of whole wheat grain. Animal Production Science 57, 2117–2124.
| Carcass composition and quality of meat from Pekin ducks finished on diets with varying levels of whole wheat grain.Crossref | GoogleScholarGoogle Scholar |
Kusumaningsih S (2001) Pemanfaatan kunyit (Curcuma domestica Val) dalam ransum broiler sebagai upaya menurunkan lemak abdominal dan kadar kolesterol. Thesis, Universitas Diponegoro, Semarang.
Lee S-O, Simons AL, Murphy PA, Hendrich S (2005) Soyasaponins lowered plasma cholesterol and increased fecal bile acids in female golden Syrian hamsters. Experimental Biology and Medicine 230, 472–478.
| Soyasaponins lowered plasma cholesterol and increased fecal bile acids in female golden Syrian hamsters.Crossref | GoogleScholarGoogle Scholar |
Maruyuni S, Wibowo E (2005) ‘Ilmu nutrisi unggas.’ (Gadjah Mada University Press. Yogyakarta)
Mazanowski A, Książkiewicz J (2004) Comprehensive evaluation of meat traits of ducks from two sire strains. Journal of Animal and Feed Sciences 13, 173–182.
| Comprehensive evaluation of meat traits of ducks from two sire strains.Crossref | GoogleScholarGoogle Scholar |
McReynolds JL, Genovese KJ, He H, Swaggerty CL, Byrd JA, Ricke SC, Nisbet DJ, Kogut MH (2009) Alfalfa as a nutritive modulator in maintaining the innate immune response during the molting process. Journal of Applied Poultry Research 18, 410–417.
| Alfalfa as a nutritive modulator in maintaining the innate immune response during the molting process.Crossref | GoogleScholarGoogle Scholar |
Montgomery R (1996) ‘Biochemistry: a case-oriented approach.’ (Mosby Incorporated)
Murray RK (2003) ‘Biokimia Harper. (Penerbit Buku Kedokteran EGC)’
Murwani R (2010) ‘Broiler modern.’ (CV. Widya Karya)
Naveen Z, Naik BR, Subramanyam B V, Reddy PM (2016) Studies on the quality of duck meat sausages during refrigeration. SpringerPlus 5, 2061
| Studies on the quality of duck meat sausages during refrigeration.Crossref | GoogleScholarGoogle Scholar |
NRC (1994) ‘Nutrient requirements of poultry: 1994.’ (National Academies Press)
Nurwantoro N, Mulyani S (2003) Dasar teknologi hasil ternak. Fakultas Peternakan Universitas Diponegoro.
Omar JMA (2015) Carcass composition and visceral organ mass of broiler chicks fed different levels of olive pulp. IUG Journal of Natural Studies 13, 175–184.
Pagala MA, Agustina D (2009) Kualitas kolesterol itik Tegal dengan pemberian seledri (Apium graviolens) dan dedak padi. Warta-Wiptek 17, 97–100.
Ponte PIP, Mendes I, Quaresma M, Aguiar MNM, Lemos JPC, Ferreira LMA, Soares MAC, Alfaia CM, Prates JAM, Fontes CMGA (2004) Cholesterol levels and sensory characteristics of meat from broilers consuming moderate to high levels of alfalfa. Poultry Science 83, 810–814.
| Cholesterol levels and sensory characteristics of meat from broilers consuming moderate to high levels of alfalfa.Crossref | GoogleScholarGoogle Scholar |
Purba M, Ketaren PP (2011) Konsumsi dan konversi pakan itik lokal jantan umur delapan minggu dengan penambahan santoquin dan vitamin E dalam pakan. JITV 16, 280–287.
Purbowati E, Sutrisno CI, Baliarti E, Budhi SPS, Lestariana W (2006) Komposisi kimia otot Longissimus dorsi dan Biceps femoris domba lokal jantan yang dipelihara di pedesaan pada bobot potong yang berbeda. Animal Production 8, 1–7.
Putra ARS, Warsito SH, Pramyrtha E (2016) Pengaruh penambahan tepung daun salam (Syzigium polianthum) dalam pakan terhadap kadar kolesterol daging itik hibrida jantan yang telah dilaserpuntkur. Jurnal Fakultas Kedokteran Hewan Universitas Airlangga 5, 1–2.
Rao AV, Agarwal S (2000) Role of antioxidant lycopene in cancer and heart disease. Journal of the American College of Nutrition 19, 563–569.
| Role of antioxidant lycopene in cancer and heart disease.Crossref | GoogleScholarGoogle Scholar |
Rhee KS, Dutson TR, Smith GC (1982) Effect of changes in intermuscular and subcutaneous fat levels on cholesterol content of raw and cooked beef steaks. Journal of Food Science 47, 1638–1642.
Risna YK (2012) Pengaruh pemberian tepung daun dan tepung buah mengkudu (Morinda citrifolia) dalam ransum terhadap kadar kolesterol daging itik. Lentera: Jurnal Ilmiah Sains Dan Teknologi 12, 152303
Rosadi I, Ismoyowati NI (2013) Kadar HDL (high density lipoprotein) dan LDL (low density lipoprotein) darah pada berbagai itik lokal betina yang pakannya disuplementasi dengan probiotik. J Ilmiah Peternakan1 (2) 597–605.
Sajimin ND, Purwantari RM (2011) Pengaruh jenis dan taraf pemberian pupuk organik pada produktivitas tanaman alfalfa (Medicago sativa L.) di Bogor Jawa Barat. In ‘Seminar nasional teknologi peternakan dan veteriner’. (Balai Penelitian Ternak: Bogor)
Slinkard K, Singleton VL (1977) Total phenol analysis: automation and comparison with manual methods. American Journal of Enology and Viticulture 28, 49–55.
SNI (2006) ‘Pakan meri (starter duck).’ (Badan Standardisasi Nasional: Indonesia)
Soeparno E (2005) ‘Ilmu dan teknologi daging.’ (Gadjah Mada University Press: Yogyakarta)
Srigandono B (2000) ‘Beternak itik pedaging.’ (PT. Trubus Agriwidya: Jakarta)
Sturkie PD (1976) ‘Avian physiology.’ 3th edn. (Spinger Verlag: New York, Heidelberg)
Subantoro R (2009) Mengenal karakter alfalfa (Medicago sativa L.). Mediagro Jurnal Ilmu Pertanian 5, 50–62.
Subhan A (2009) Pengaruh penggunaan sagu kukus (Metroxylon Spp) dan tepung keong mas (Pomacea Spp) sebagai pengganti jagung kuning dalam pakan terhadap penampilan itik jantan alabio, Mojosari dan hasil persilangannya. Thesis, Universitas Gadjah Mada, Yogyakarta.
Suciani KWP, Sumardani NLG, Bidura IGNG, Kayana IGN, Lindawati SA (2011) Penambahan multi enzim dan ragi tape dalam ransum berserat tinggi (pod-kakao) untuk menurunkan kolesterol daging broiler. Jurnal Veteriner 12, 69–76.
Suharti S, Banowati A, Hermana W, Wiryawan KG (2008) Komposisi dan kandungan kolesterol karkas ayam broiler diare yang diberi tepung daun salam (Syzygium polyanthum Wight) dalam Ransum. Media Peternakan 31, 138–145.
Sukada IK, Bidura IGNG, Warmadewi DA (2007) Pengaruh penggunaan pollard, kulit kacang kedelai, dan pod kakao terfermentasi dengan ragi tape terhadap karkas dan kadar kolesterol daging itik bali jantan. Majalah Ilmiah Peternakan 10, 1–15.
Sukmawati NMS, Wirawan IW, Trisnadewi AAAS, Yadnya TGB (2014) Lemak tubuh dan profil lipida darah itik bali jantan yang diberi ransum mengandung daun ubi jalar ungu (Ipomoea Batatas L) disuplementasi Starbio dan Pignox (Starpig). Majalah Ilmiah Peternakan 17, 164197
| Lemak tubuh dan profil lipida darah itik bali jantan yang diberi ransum mengandung daun ubi jalar ungu (Ipomoea Batatas L) disuplementasi Starbio dan Pignox (Starpig).Crossref | GoogleScholarGoogle Scholar |
Summers JD (2004) Broiler carcass composition. Poultry Industry Council for Research and Education Guelph.
Suwignyo B, Izzati F, Astuti A, Rini EA (2020) Nutrient content of alfalfa (Medicago sativa L.) regrowth in different fertilizers and lighting. In ‘IOP conference series: earth and environmental science’. pp. 12035. (IOP Publishing)
Suwignyo B, Rini EA, Fadli MK, Ariyadi B (2021a) Effects of alfalfa (Medicago sativa L.) supplementation in the diet on the growth, small intestinal histomorphology, and digestibility of hybrid ducks. Veterinary World 14, 2719–2726.
| Effects of alfalfa (Medicago sativa L.) supplementation in the diet on the growth, small intestinal histomorphology, and digestibility of hybrid ducks.Crossref | GoogleScholarGoogle Scholar |
Suwignyo B, Arifin L, Umami N, Muhlisin M, Suhartanto B (2021b) The performance and genetic variation of first and second generation tropical alfalfa (Medicago sativa). Biodiversitas Journal of Biological Diversity 22, 3265–3270.
Suwignyo B, Suhartanto B, Noviandi CT, Umami N, Suseno N, Prasetiyono BWHE (2017) Generative plant characteristics alfalfa (Medicago sativa L.) on different levels of dolomite and lighting duration. In ‘Proceeding of the 1st international conference on tropical agriculture’. pp. 353–361. (Springer) https://doi.org/10.1007/978-3-319-60363-6_34
Swatland HJ (1984) ‘Structure and development of meat animals.’ (Prentice Hall, Inc.)
Tugiyanti E, Heriyanto S, Syamsi AN (2016) Pengaruh tepung daun sirsak (Announa muricata L) terhadap karakteristik lemak darah dan daging itik Tegal jantan. Buletin Peternakan 40, 211–218.
| Pengaruh tepung daun sirsak (Announa muricata L) terhadap karakteristik lemak darah dan daging itik Tegal jantan.Crossref | GoogleScholarGoogle Scholar |
Widyamanda LP, Ismadi VDYB, Estiningdriati I (2013) Pengaruh penambahan bangle (Zingiber cas-sumunar) dalam ransum terhadap total lipid dan kolesterol hati pada ayam broiler. Animal Agriculture Journal 2, 183–190.
Wijaya VG, Ismoyowati SDM (2013) Kajian kadar kolesterol dan trigliserida darah berbagai itik lokal yang pakannya disuplementasi dengan probiotik. Jurnal Ilmu Peternakan 1, 661–668.
Wolever TM, Hegele RA, Connelly PW, Ransom TP, Story JA, Furumoto EJ, Jenkins DJ (1997) Long-term effect of soluble-fiber foods on postprandial fat metabolism in dyslipidemic subjects with apo E3 and apo E4 genotypes. The American Journal of Clinical Nutrition 66, 584–590.
| Long-term effect of soluble-fiber foods on postprandial fat metabolism in dyslipidemic subjects with apo E3 and apo E4 genotypes.Crossref | GoogleScholarGoogle Scholar |
Yovina S (2012) ‘Kolesterol.’ (Pinang Merah Publisher: Yogyakarta)
Zhu C, Jiang ZY, Jiang SQ, Zhou GL, Lin YC, Chen F, Hong P (2012) Maternal energy and protein affect subsequent growth performance, carcass yield, and meat color in Chinese Yellow broilers. Poultry Science 91, 1869–1878.
| Maternal energy and protein affect subsequent growth performance, carcass yield, and meat color in Chinese Yellow broilers.Crossref | GoogleScholarGoogle Scholar |
