Register      Login
Animal Production Science Animal Production Science Society
Food, fibre and pharmaceuticals from animals
RESEARCH ARTICLE

Replacement of rice straw with cassava-top silage on rumen ecology, fermentation and nutrient digestibilities in dairy steers

Bounnaxay Viennasay A , Metha Wanapat A C , Khampanat Phesatcha B , Burarat Phesatcha A and Thiwakorn Ampapon A
+ Author Affiliations
- Author Affiliations

A Tropical Feed Resources Research and Development Centre (TROFREC), Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, Thailand.

B Department of Animal Science, Faculty of Agriculture and Technology, Nakhon Phanom University, Nakhon Phanom 48000, Thailand.

C Corresponding author. Email: metha@kku.ac.th

Animal Production Science 59(5) 906-913 https://doi.org/10.1071/AN17477
Submitted: 17 July 2017  Accepted: 22 April 2018   Published: 6 June 2018

Abstract

Conserving good source of roughage for dry-season feeding of ruminants is of the utmost importance. Therefore, cassava-top silage (CTS) was prepared and was studied for its feeding level and nutritive value in dairy steers. Four rumen-fistulated dairy steers (75% Holstein Friesian × 25% Thai Native), ~3 years old with 209 ± 50 kg liveweight, were randomly assigned according to a 4 × 4 Latin square design to study the effect of CTS on feed intake, nutrient digestibility and rumen fermentation efficiency. The treatments were four different feeding ratios of CTS to rice straw, as follows: 0 : 100, 30 : 70, 60 : 40 and 100 : 0 respectively. Increasing the ratio of CTS to rice straw in the feed remarkably reduced the rumen protozoal population (P < 0.01), thus subsequently decreasing rumen methane production (P < 0.01), whereas other rumen microorganisms remained similar among treatments. Correspondingly, rumen propionate production was enhanced (P < 0.01) by increasing the CTS to rice ratio in the feed, and the acetate : propionate ratio decreased (P < 0.01). Furthermore, rumen ammonia nitrogen and blood urea nitrogen increased (P < 0.01) with an increasing proportion of CTS in the feed, whereas nitrogen retention was highest when the proportion of CTS in the feed was 100%. Although the feed DM intakes were similar, apparent digestibilities of DM, organic matter, crude protein as well as neutral detergent fibre and acid detergent fibre were significantly improved by the increase in the percentage of CTS in the feed. It could be concluded that including CTS in the feed at a level of 60–100% improved the rumen fermentation, nitrogen balance and nutrient digestibilities, and is highly recommended for use in the ruminant production in the tropics, as it is a practically simple-to-prepare on-farm feeding intervention.

Additional keywords: feed resource, feeding, manipulation.


References

Ali M, Cone JW, Van Duinkerken G, Klop A, Blok MC, Bruinenberg M, Hendriks WH (2016) Variation between individual cows in in situ rumen degradation characteristics of maize and grass silages. NJAS Wageningen Journal of Life Sciences 78, 167–173.
Variation between individual cows in in situ rumen degradation characteristics of maize and grass silages.Crossref | GoogleScholarGoogle Scholar |

Anantasook N, Wanapat M (2012) Influence of rain tree pod meal supplementation on rice straw based diets using in vitro gas fermentation technique. Asian-Australasian Journal of Animal Sciences 25, 325–334.
Influence of rain tree pod meal supplementation on rice straw based diets using in vitro gas fermentation technique.Crossref | GoogleScholarGoogle Scholar |

AOAC (2012) ‘Official methods of analysis.’ 19th edn. (Association of Official Analytical Chemists: Gaithersburg, MD)

Bolhuis GG (1954) The toxicity of cassava root. Netherlands Journal of Agricultural Science 2, 176–186.

Brien GM, Taylor AJ, Poulter NH (1991) Improved enzymic assay for cyanogens in fresh and processed cassava. Journal of the Science of Food and Agriculture 56, 277–289.
Improved enzymic assay for cyanogens in fresh and processed cassava.Crossref | GoogleScholarGoogle Scholar |

Cherdthong A, Wanapat M, Wachirapakorn C (2011) Influence of urea calcium mixture supplementation on ruminal fermentation characteristics of beef cattle fed on concentrates containing high levels of cassava chips and rice straw. Animal Feed Science and Technology 163, 43–51.
Influence of urea calcium mixture supplementation on ruminal fermentation characteristics of beef cattle fed on concentrates containing high levels of cassava chips and rice straw.Crossref | GoogleScholarGoogle Scholar |

Crocker CL (1967) Rapid determination of urea nitrogen in serum or plasma without deproteinization. The American Journal of Medical Technology 33, 361–365.

Denman SE, McSweeney CS (2006) Development of a real-time PCR assay for monitoring anaerobic fungal and cellulolytic bacterial populations within the rumen. FEMS Microbiology Ecology 58, 572–582.
Development of a real-time PCR assay for monitoring anaerobic fungal and cellulolytic bacterial populations within the rumen.Crossref | GoogleScholarGoogle Scholar |

Erdman RA, Proctor GH, Vandersall JH (1986) Effect of rumen ammonia concentration on in situ rate and extent of digestion of feedstuffs. Journal of Dairy Science 69, 2312–2320.
Effect of rumen ammonia concentration on in situ rate and extent of digestion of feedstuffs.Crossref | GoogleScholarGoogle Scholar |

Galyean M (1989) ‘Laboratory procedure in animal nutrition research.’ (Department of Animal and Range Science, New Mexico State University: Las Cruces, New Mexico)

Giang NTT, Wanapat M, Phesatcha K, Kang S (2016) Level of Leucaena leucocephala silage feeding on intake, rumen fermentation, and nutrient digestibility in dairy steers. Tropical Animal Health and Production 48, 1057–1064.
Level of Leucaena leucocephala silage feeding on intake, rumen fermentation, and nutrient digestibility in dairy steers.Crossref | GoogleScholarGoogle Scholar |

Hammond AC (1983) Effect of dietary protein level, ruminal protein solubility and time after feeding on plasma urea nitrogen and the relationship of plasma urea nitrogen to other ruminal and plasma parameters. Journal of Animal Science 57, 435

Hennessy DW, Nolan JV (1988) Nitrogen kinetics in cattle fed a mature subtropical grass hay with and without protein meal supplementation. Crop and Pasture Science 39, 1135–1150.
Nitrogen kinetics in cattle fed a mature subtropical grass hay with and without protein meal supplementation.Crossref | GoogleScholarGoogle Scholar |

Hobson PN (1965) Continuous culture of some anaerobic and facutatively anaerobic rumen bacteria. Journal of General Microbiology 38, 80–167.
Continuous culture of some anaerobic and facutatively anaerobic rumen bacteria.Crossref | GoogleScholarGoogle Scholar |

Hobson PN, Stewart CS (1997) ‘The rumen microbial ecosystem.’ (Chapman & Hall: New York)

Hungate RE (1966) ‘The rumen and its microbes.’ (Academic Press, Inc.: New York)

Hungate RE (1969) A role tube method for cultivation of strict anaerobes. In ‘Method in microbiology’. (Eds JR Norris, DW Ribbons) p. 313. (Academic Press: New York)

Kamra DN (2005) Rumen microbial ecosystem. Current Science 89, 124–135.

Kazemi-Bonchenari M, Rezayazdi K, Nikkhah A, Kohram H, Dehghan-Banadaky M (2010) The effects of different levels of sodium caseinate on rumen fermentation pattern, digestibility and microbial protein synthesis of Holstein dairy cows. African Journal of Biotechnology 9, 1990–1998.
The effects of different levels of sodium caseinate on rumen fermentation pattern, digestibility and microbial protein synthesis of Holstein dairy cows.Crossref | GoogleScholarGoogle Scholar |

Khang DN, Wiktorsson H (2004) Effects of fresh cassava tops on rumen environment parameters, thyroid gland hormones and liver enzymes of local yellow cattle fed urea-treated fresh rice straw. Tropical Animal Health and Production 36, 751–762.
Effects of fresh cassava tops on rumen environment parameters, thyroid gland hormones and liver enzymes of local yellow cattle fed urea-treated fresh rice straw.Crossref | GoogleScholarGoogle Scholar |

Kobawila SC, Louembe D, Keleke S, Traore AS (2003) Aspects physico-chimiques et biochimiques de l fermentation des feuilles de manioc en ntoba mbodi. Procédés Biologique et Alimentaire 1, 106–119.

Koike S, Kobayashi Y (2001) Development and use of competitive PCR assays for the rumen cellulolytic bacteria: Fibrobacter succinogenes, Ruminococcus albus and Ruminococcus flavefaciens. FEMS Microbiology Letters 204, 361–366.
Development and use of competitive PCR assays for the rumen cellulolytic bacteria: Fibrobacter succinogenes, Ruminococcus albus and Ruminococcus flavefaciens.Crossref | GoogleScholarGoogle Scholar |

Lapierre H, Lobley GE (2001) Nitrogen recycling in the ruminant: a review. Journal of Dairy Science 84, E223–E236.
Nitrogen recycling in the ruminant: a review.Crossref | GoogleScholarGoogle Scholar |

Limon Loeza RL (1992) Ensilage of cassava products and their use as animal feed. Paper 95. In ‘Roots, tubers, plantains and bananas in animal feeding. Animal Production and Health Publication’. (Eds D Machin, AW Speedy) pp. 99–110. (FAO: Rome, Italy)

Louembe D, Malonga M, Kobawila SC, Mavoungou O (1997) Evolution de la teneur en composes cyanés des tubercules de manioc au cours du rouissage-activité linamarasique de bactéries lactiques. MAN Microbiologie, aliments, nutrition 15, 53–60.

Makkar HPS, Blümmel M, Becker K (1995) In vitro effects of and interactions between tannins and saponins and fate of tannins in the rumen. Journal of the Science of Food and Agriculture 69, 481–493.
In vitro effects of and interactions between tannins and saponins and fate of tannins in the rumen.Crossref | GoogleScholarGoogle Scholar |

McAllister TA, Newbold CJ (2008) Redirecting rumen fermentation to reduce methanogenesis. Animal Production Science 48, 7–13.
Redirecting rumen fermentation to reduce methanogenesis.Crossref | GoogleScholarGoogle Scholar |

Moss R, Jouany IP, Newbold J (2000) Methane production by ruminants: its contribution to global warming. Annales de Zootechnie 49, 231–253.
Methane production by ruminants: its contribution to global warming.Crossref | GoogleScholarGoogle Scholar |

Oni AO, Sowande OS, Oni OO, Aderinboye RY, Dele PA, Ojo VOA, Onwuka CFI (2014) Effect of additives on fermentation of cassava leaf silage and ruminal fluid of West African dwarf goats. Archivos de Zootecnia 63, 449–459.
Effect of additives on fermentation of cassava leaf silage and ruminal fluid of West African dwarf goats.Crossref | GoogleScholarGoogle Scholar |

Petterson K (1988) Ensiling of forages. Factors affecting silage fermentation and quality. Dissertation, Swedish University of Agricultural Sciences. Report 179. Uppsala, Sweden.

Ravindran V (1992) Preparation of cassava leaf products and their use as animal feed. In ‘Roots, tubers, plantains and bananas in animal feeding. FAO Animal Production and Health. Publication 95’. (Eds D Machin, AW Speedy) pp. 111–126. (FAO: Rome, Italy)

Ravindran V, Blair R (1992) Feed resources for poultry production in Asia and the Pacific. II. Plant protein sources. World’s Poultry Science Journal 48, 205–231.
Feed resources for poultry production in Asia and the Pacific. II. Plant protein sources.Crossref | GoogleScholarGoogle Scholar |

Samuel M, Sagathevan S, Thomas J, Mathen G (1997) An HPLC method for estimation of volatile fatty acids in ruminal fluid. The Indian Journal of Animal Sciences 67, 805–807.

Santiago AMF, Campos JMDS, Oliveira ASD, Valadares Filho SDC, Santos SA, Souza SMD, Santiago IF (2013) Urea in sugarcane-based diets for dairy cows. Revista Brasileira de Zootecnia 42, 456–462.
Urea in sugarcane-based diets for dairy cows.Crossref | GoogleScholarGoogle Scholar |

SAS (Statistical Analysis System) (2013) ‘User’s guide: statistics. Version 9.’ 4th edn. (SAS Institute Inc.: Cary, NC)

Steel RGD, Torrie JH (1980) ‘Principles and procedures of statistics: a biometrical approach.’ 2nd edn. (McGraw-Hill Book Company: New York)

Suksombat W, Mernkrathoke P (2005) Feeding of whole sugar cane to dairy cattle during the dry season. Asian-Australasian Journal of Animal Sciences 18, 345–349.
Feeding of whole sugar cane to dairy cattle during the dry season.Crossref | GoogleScholarGoogle Scholar |

Tavendale MH, Meagher LP, Pacheco D, Walker N, Attwood GT, Sivakumaran S (2005) Methane production from in vitro rumen incubations with Lotus pedunculatus and Medicago sativa, and effects of extractable condensed tannin fractions on methanogenesis. Animal Feed Science and Technology 123–124, 403–419.
Methane production from in vitro rumen incubations with Lotus pedunculatus and Medicago sativa, and effects of extractable condensed tannin fractions on methanogenesis.Crossref | GoogleScholarGoogle Scholar |

Tewe OO (1991) Detoxification of cassava products and effects of residue toxins on consuming animals. In ‘Roots, tubers, plantains and bananas in animal feeding. FAO Animal Production and Health Publication 95’. (Eds D Machin, S Nyvold) pp. 85–91. (FAO: Rome, Italy)

Thornton RF (1970) Factors affecting the urinary excretion of urea nitrogen in cattle. II. The plasma urea nitrogen concentration. Crop and Pasture Science 21, 145–152.
Factors affecting the urinary excretion of urea nitrogen in cattle. II. The plasma urea nitrogen concentration.Crossref | GoogleScholarGoogle Scholar |

Van Man N, Wiktorsson H (2001) Cassava tops ensiled with or without molasses as additive effects on quality, feed intake and digestibility by heifers. Asian-Australasian Journal of Animal Sciences 14, 624–630.
Cassava tops ensiled with or without molasses as additive effects on quality, feed intake and digestibility by heifers.Crossref | GoogleScholarGoogle Scholar |

Van Man N, Wiktorsson H (2002) Effect of molasses on nutritional quality of cassava and gliricidia tops silage. Asian-Australasian Journal of Animal Sciences 15, 1294–1299.
Effect of molasses on nutritional quality of cassava and gliricidia tops silage.Crossref | GoogleScholarGoogle Scholar |

Van Soest PJ (1994) ‘Nutritional ecology of the ruminant.’ (Cornell University Press: New York)

Van Soest PJ, Robertson JB, Lewis BA (1991) Methods for dietary fiber neutral detergent fiber and non-starch polysaccharides in relation to animal nutrition. Journal of Dairy Science 74, 3583–3597.
Methods for dietary fiber neutral detergent fiber and non-starch polysaccharides in relation to animal nutrition.Crossref | GoogleScholarGoogle Scholar |

Wanapat M (2000) Rumen manipulation to increase the efficient use of local feed resources and productivity of ruminants in the tropics. Asian-Australasian Journal of Animal Sciences 13, 59–67.

Wanapat M (2002) The role of cassava hay as animal feed. In ‘Research and development in Asia: exploring new opportunities for an ancient crop. Proceedings of the 7th regional cassava workshop’. (Ed. RH Howeler) (CIAT)

Wanapat M, Cherdthong A (2009) Use of real-time PCR technique in studying rumen cellulolytic bacteria population as affected by level of roughage in swamp buffalo. Current Microbiology 58, 294–299.
Use of real-time PCR technique in studying rumen cellulolytic bacteria population as affected by level of roughage in swamp buffalo.Crossref | GoogleScholarGoogle Scholar |

Wanapat M, Pimpa O (1999) Effect of ruminal NH3-N levels on ruminal fermentation, purine derivatives, digestibility and rice straw intake in swamp buffaloes. Asian-Australasian Journal of Animal Sciences 12, 904–907.
Effect of ruminal NH3-N levels on ruminal fermentation, purine derivatives, digestibility and rice straw intake in swamp buffaloes.Crossref | GoogleScholarGoogle Scholar |

Wanapat M, Petlum A, Pimpa O (2000) Supplementation of cassava hay to replace concentrate use in lactating Holstein Friesian crossbreds. Asian-Australasian Journal of Animal Sciences 13, 600–604.
Supplementation of cassava hay to replace concentrate use in lactating Holstein Friesian crossbreds.Crossref | GoogleScholarGoogle Scholar |

Wanapat M, Kang S, Polyorach S (2013a) Development of feeding systems and strategies of supplementation to enhance rumen fermentation and ruminant production in the tropics. Journal of Animal Science and Biotechnology 4, 32
Development of feeding systems and strategies of supplementation to enhance rumen fermentation and ruminant production in the tropics.Crossref | GoogleScholarGoogle Scholar |

Wanapat M, Anantasook N, Rowlinson P, Pilajun R, Gunun P (2013b) Effect of carbohydrate sources and levels of cotton seed meal in concentrate on feed intake, nutrient digestibility, rumen fermentation and microbial protein synthesis in young dairy bulls. Asian-Australasian Journal of Animal Sciences 26, 529–536.
Effect of carbohydrate sources and levels of cotton seed meal in concentrate on feed intake, nutrient digestibility, rumen fermentation and microbial protein synthesis in young dairy bulls.Crossref | GoogleScholarGoogle Scholar |