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RESEARCH ARTICLE

A comparison of the effects of post-mortem aging on breast meat from Cobb 500 and Hubbard ISA broilers

J. L. M. Mello A C , R. A. Souza A B , G. C. Paschoalin A , F. B. Ferrari A , B. M. Machado A , A. Giampietro-Ganeco A , P. A. Souza A and H. Borba A
+ Author Affiliations
- Author Affiliations

A São Paulo State University – UNESP, Department of Technology, Via de Acesso Professor Paulo Donato Castellane, s/n, Zona Rural, 14884-900, Jaboticabal, São Paulo, Brazil.

B University of São Paulo – USP, School of Animal Science and Food Engineering, Avenuenida Duque de Caxias Norte, No. 225, 13635-900, Pirassununga, São Paulo, Brazil.

C Corresponding author. Email: julianalolli@zootecnista.com.br

Animal Production Science 58(10) 1922-1931 https://doi.org/10.1071/AN16603
Submitted: 7 September 2016  Accepted: 24 March 2017   Published: 22 May 2017

Abstract

We compared the physical and chemical characteristics of the pectoralis major muscle from Cobb 500 and Hubbard ISA broilers and evaluated the effect of aging process for up to 7 days on meat quality. We used breast samples from male Cobb 500 (42 days of age; n = 60) and Hubbard ISA (85 days of age; n = 60) broilers. Twenty samples of each genotype were analysed 4 h post-slaughter (Control group). Another 20 samples of each genotype were aged in an incubator (2 ± 0.5°C) for 3 and for 7 days. Breast fillets were 23% heavier in the Cobb 500 group (306 g vs 248 g; P = 0.0009). Before aging, meat from Hubbard ISA broilers had higher (P < 0.001) water-holding capacity, which promotes the production of less exudate and possibly reduces nutritional losses during storage, which may be beneficial to the poultry industry. Despite being initially less tender, breast meat from Hubbard ISA broilers showed, during the aging process, a reduction (P < 0.05) of shear force (48.46–15.04 N), total collagen amount (6.0–4.8 g/kg) and myofibrillar fragmentation index (150.17–97.42) and had the same (P = 0.134) fat concentration (0.87 g/100 g) as that of breast meat from Cobb 500 broilers (0.93 g/100 g, respectively). Breast meat from Hubbard ISA broilers had a higher (P < 0.001) polyunsaturated fatty acid concentration, especially docosahexaenoic acid, which is beneficial to human health. Aging breast fillets for 3 days at 2°C is sufficient to tenderise the meat without reducing its juiciness, which suggests that the aging process can add value to free-range meat.

Additional keywords: chicken, free-range poultry, meat quality, PUFA, tenderness.


References

Aguda AH, Panwar P, Du X, Nguyen NT, Brayer GD, Bromme D (2014) Structural basis of collagen fiber degradation by cathepsin K. Proceedings of the National Academy of Sciences of the United States of America 111, 17474–17479.
Structural basis of collagen fiber degradation by cathepsin K.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2cXhvFKmu7rM&md5=8bdefab770a5d34d0c993719a836dda8CAS |

AOAC (2005) ‘Official methods of analysis.’ 18th edn. (Association of Analytical Chemists: Washington, DC)

Bailey AJ, Sims TJ (1977) Meat tenderness: distribution of molecular species of collagen in bovine muscle. Journal of the Science of Food and Agriculture 28, 565–570.
Meat tenderness: distribution of molecular species of collagen in bovine muscle.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaE2sXltlantr0%3D&md5=03be6586fa2044d3e818725480626100CAS |

Baldwin DE (2012) Sous vide cooking: a review. International Journal of Gastronomy and Food Science 1, 15–30.

Berri C, Wacrenier N, Millet NLE, Bihan-Duval E (2001) Effect of selection for improved body composition on muscle and meat characteristics of broilers from experimental and commercial lines. Poultry Science 80, 833–838.
Effect of selection for improved body composition on muscle and meat characteristics of broilers from experimental and commercial lines.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXovFKlu7g%3D&md5=c3481c6d2513eef85a160cced5aec5a6CAS |

Berri C, Le Bihan-Duval E, Debut M, Sante-Lhoutellier V, Baeza E, Gigaud V, Jego Y, Duclos MJ (2007) Consequence of muscle hypertrophy on characteristics of pectoralis major muscle and breast meat quality of broiler chickens. Journal of Animal Science 85, 2005–2011.
Consequence of muscle hypertrophy on characteristics of pectoralis major muscle and breast meat quality of broiler chickens.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXot1Ohtbg%3D&md5=084a8f6fe981f9ff7fbe32bff8e4eafbCAS |

Bligh GE, Dyer JW (1959) A rapid method of total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology 37, 911–917.
A rapid method of total lipid extraction and purification.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaG1MXhtVSgt70%3D&md5=4e60a5c754d3f3b9f9701b07dd6a3f60CAS |

Brazilian Association of Animal Protein (ABPA) (2016) Annual report 2016. Available at http://abpa-br.com.br/setores/avicultura/publicacoes/relatorios-anuais [Verified 25 November 2016]

Brenes A, Viveros A, Gon I, Centeno C, Sa’yago-Ayerdy SG, Arija I (2008) Effect of grape pomace concentrate and vitamin E on digestibility of polyphenols and antioxidant activity in chickens. Poultry Science 87, 307–316.
Effect of grape pomace concentrate and vitamin E on digestibility of polyphenols and antioxidant activity in chickens.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXitFWls7s%3D&md5=49a6b0bf920cb95433ca041fdb2d5d1aCAS |

Brewer S, Novakofski J (2008) Consumer sensory evaluations of aging effects on beef quality. Journal of Food Science 73, S78–S82.
Consumer sensory evaluations of aging effects on beef quality.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXhtlWnurc%3D&md5=2918a555e7da8686ff487e00550fea31CAS |

Castellini C, Mugnai CAND, Dal Bosco A (2002) Effect of organic production system on broiler carcass and meat quality. Meat Science 60, 219–225.
Effect of organic production system on broiler carcass and meat quality.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BC3MbnsFOhsg%3D%3D&md5=f7c000682b584ec021726e97eb73931dCAS |

Castellini C, Dal Bosco A, Mugnai C, Pedrazzoli M (2006) Comparison of two chicken genotypes organically reared: oxidative stability and other qualitative traits of the meat. Italian Journal of Animal Science 5, 29–42.
Comparison of two chicken genotypes organically reared: oxidative stability and other qualitative traits of the meat.Crossref | GoogleScholarGoogle Scholar |

Castellini C, Berri C, Le Bihan-Duval E, Martino G (2008) Qualitative attributes and consumer perception of organic and free-range poultry meat. World’s Poultry Science Journal 64, 500–512.
Qualitative attributes and consumer perception of organic and free-range poultry meat.Crossref | GoogleScholarGoogle Scholar |

Cortinas L, Barroeta A, Villaverde C, Galobart J, Guardiola F, Baucells MD (2005) Influence of the dietary polyunsaturation level on chicken meat quality: lipid oxidation. Poultry Science 84, 48–55.
Influence of the dietary polyunsaturation level on chicken meat quality: lipid oxidation.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD2M%2FlslGgsw%3D%3D&md5=80078b70788c8977b9d6d5e85bab7b5cCAS |

Culler RD, Parrish FC, Smith GC, Cross HR (1978) Relationship of myofibril fragmentation index to certain chemical, physical and sensory characteristics of bovine Longissimus muscle. Journal of Food Science 43, 1177–1180.
Relationship of myofibril fragmentation index to certain chemical, physical and sensory characteristics of bovine Longissimus muscle.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaE1cXlt1Oqs7s%3D&md5=307fd26bc0e9c2b267c254a089183a50CAS |

Dabes AC (2001) Propriedades da carne fresca. Revista Nacional da Carne 25, 32–40.

de la Torre CAL, Conte-Júnior CA, Canto ACVCS, Monteiro MLG, Lima BRCC, Mársico ET, Mano SB, Franco RM (2012) Biochemical changes in alternative poultry meat during refrigerated storage. Revista Brasileira de Ciência Veterinária 19, 195–200.
Biochemical changes in alternative poultry meat during refrigerated storage.Crossref | GoogleScholarGoogle Scholar |

Debut M, Berri C, Baeza E, Sellier N, Arnould C, Guemene D, Jehl N, Boutten B, Jego Y, Beaumont C, Le Bihan-Duval E (2003) Variation of chicken technological meat quality in relation to genotype and preslaughter stress conditions. Poultry Science 82, 1829–1838.
Variation of chicken technological meat quality in relation to genotype and preslaughter stress conditions.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD2c%2FitVantA%3D%3D&md5=14c9c073faec101a498ba4620d2fa3cdCAS |

Dugan MER, Vahmani P, Turner TD, Mapiye C, Juárez M, Prieto N, Beaulieu AD, Zijlstra RT, Patience JF, Aalhus JL (2015) Pork as a source of omega-3 (n-3) fatty acids. Journal of Clinical Medicine 4, 1999–2011.
Pork as a source of omega-3 (n-3) fatty acids.Crossref | GoogleScholarGoogle Scholar |

Fanatico AC, Cavitt LC, Pillai PB, Emmert JL, Owens CM (2005) Evaluation of slower-growing broiler genotypes grown with and without outdoor access: Meat Quality. Poultry Science 84, 1785–1790.
Evaluation of slower-growing broiler genotypes grown with and without outdoor access: Meat Quality.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD28%2FnvVajsg%3D%3D&md5=7cd4a26442f5197a72591b7fe866c5d3CAS |

Fanatico AC, Pillai PB, Cavitt LC, Emmert JL, Meullenet JF, Owens CM (2006) Evaluation of slower-growing broiler genotypes grown with and without outdoor access: sensory attributes. Poultry Science 85, 337–343.
Evaluation of slower-growing broiler genotypes grown with and without outdoor access: sensory attributes.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD287js1aksQ%3D%3D&md5=3472d1d41ff12b0e0bae3febd450131fCAS |

Fanatico AC, Pillai PB, Emmert JL, Owens CM (2007) Meat quality of slow-growing chicken genotypes fed low-nutrient or standard diets and raised indoor or with outdoor access. Poultry Science 86, 2245–2255.
Meat quality of slow-growing chicken genotypes fed low-nutrient or standard diets and raised indoor or with outdoor access.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXhtF2hsL%2FL&md5=af865fd4d27ac0f5b5aa2e318e7f6e9dCAS |

Farmer LJ, Perry GC, Lewis PD, Nute GR, Piggott JR, Patterson RLS (1997) Responses of two genotypes of chicken to the diets and stocking densities of conventional UK and label rouge production systems. II. Sensory attributes. Meat Science 47, 77–93.
Responses of two genotypes of chicken to the diets and stocking densities of conventional UK and label rouge production systems. II. Sensory attributes.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BC3MbnsFSqsw%3D%3D&md5=fccbe3b9ee982a290784f57372430328CAS |

Fernandez X, Sante V, Baeza E, Le Bihan Duval E, Berri C, Remignon H, Babile R, Le Pottier G, Millet N, Berge P, Astruc T (2001) Post mortem muscle metabolism and meat quality in three genetic types of turkey. British Poultry Science 42, 462–469.
Post mortem muscle metabolism and meat quality in three genetic types of turkey.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXnsFSisrk%3D&md5=06a2820456cadeb6fbd004a0f6343336CAS |

Funaro A, Cardenia V, Petracci M, Rimini S, Rodriguez-Estrada MT, Cavani C (2014) Comparison of meat quality characteristics and oxidative stability between conventional and free-range chickens. Poultry Science 93, 1511–1522.
Comparison of meat quality characteristics and oxidative stability between conventional and free-range chickens.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2cXpt1Wjs7w%3D&md5=a7d836c9a1400f4bf1357cc536205cafCAS |

Gordon SH, Charles DR (2002) ‘Niche and organic chicken products.’ (Nottingham University Press: Nottingham, UK)

Gornall AG, Bardawill CJ, David MM (1949) Determination of serum protein by means of the biuret reaction. The Journal of Biological Chemistry 177, 751–766.

Grunert KG, Bredahl L, Brunso K (2004) Consumer perception of meat quality and implications for product development in the meat sector – a review. Meat Science 66, 259–272.
Consumer perception of meat quality and implications for product development in the meat sector – a review.Crossref | GoogleScholarGoogle Scholar |

Hamm R (1961) Biochemistry of meat hydratation. Advances in Food Research 10, 355–463.
Biochemistry of meat hydratation.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaF3MXltVagsQ%3D%3D&md5=8bc9c2dd2a90c6e8c362c2b538c4d980CAS |

Hartree EF (1972) Determination of protein: a modification of the Lowry method that gives a linear photometric response. Analytical Biochemistry 48, 422–427.
Determination of protein: a modification of the Lowry method that gives a linear photometric response.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaE38XksVGjur0%3D&md5=df18a08a68c89db1aa8f80dca94c95b5CAS |

Honikel KO (1987) The water binding of meat. Fleischwirttsch 67, 1098–1102.

Howe P, Meyer B, Record S, Baghurst K (2006) Dietary intake of long-chain omega-3 polyunsaturated fatty acids: Contribution of meat sources. Nutrition (Burbank, Los Angeles County, Calif.) 22, 47–53.
Dietary intake of long-chain omega-3 polyunsaturated fatty acids: Contribution of meat sources.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XhtFWiurs%3D&md5=b550629e345d0b8b5d7e7b3b4c0ca18fCAS |

Koohmaraie M, Babiker AS, Schroeder AL, Merkel RA, Dutson TR (1988) Acceleration of postmortem tenderization in ovine carcasses through activation of Ca2-dependent proteases. Journal of Food Science 53, 1638–1641.
Acceleration of postmortem tenderization in ovine carcasses through activation of Ca2-dependent proteases.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL1MXls1agsg%3D%3D&md5=9a719acde45849865beb6e577b11aa8eCAS |

Koohmaraie M, Whipple G, Crouse JD (1990) Acceleration of postmortem tenderization in lamb and Brahman cross beef carcasses through infusion of calcium chloride. Journal of Animal Science 68, 1268–1278.

Koohmaraie M, Kent MP, Shackelford SD, Veiseth E, Wheeler TL (2002) Meat tenderness and muscle growth: is there any relationship? Meat Science 62, 345–352.
Meat tenderness and muscle growth: is there any relationship?Crossref | GoogleScholarGoogle Scholar |

Lawrie RA (1985) ‘Meat science.’ 4th edn. (Pergamon Press: Oxford, New York, Toronto, Sydney, Paris, Frankfurt)

Le-Bihan Duval E, Debut M, Berri CM, Sellier N, Santé-Lhoutellier V, Jégo Y, Beaumont C (2008) Chicken meat quality: genetic variability and relationship with growth and muscle characteristics. BMC Genetics 9, 53
Chicken meat quality: genetic variability and relationship with growth and muscle characteristics.Crossref | GoogleScholarGoogle Scholar |

Ledward DA (1992) Colour of raw and cooked meat. In ‘The chemistry of muscle-based foods’. (Eds DE Johnson, MK Knight, DA Ledward) pp. 128–144. (Royal Society of Chemistry: Cambridge)

Li P, Wang T, Mao Y, Zhang Y, Niu L, Liang R, Zhu L, Luo X (2014) Effect of ultimate pH on postmortem myofibrillar protein degradation and meat quality characteristics of Chinese yellow crossbreed cattle. The Scientific World Journal 2014, 174253
Effect of ultimate pH on postmortem myofibrillar protein degradation and meat quality characteristics of Chinese yellow crossbreed cattle.Crossref | GoogleScholarGoogle Scholar |

Listrat A, Lebret B, Louveau I, Astruc T, Bonnet M, Lefaucheur L, Picard B, Bugeon J (2016) How muscle structure and composition influence meat and flesh quality. The Scientific World Journal 2016, 3182746
How muscle structure and composition influence meat and flesh quality.Crossref | GoogleScholarGoogle Scholar |

Lonergan SM, Deeb N, Fedler CA, Lamont SJ (2003) Breast meat quality and composition in unique chicken populations. Poultry Science 82, 1990–1994.
Breast meat quality and composition in unique chicken populations.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD2c%2FitValtA%3D%3D&md5=24e42b4bcb42440be0dc5b30c1b23075CAS |

Lyon CE, Lyon BG, Dickens JA (1998) Effects of carcass stimulation, deboning time, and marination on color and texture of broiler breast meat. Journal of Applied Poultry Research 7, 53–60.
Effects of carcass stimulation, deboning time, and marination on color and texture of broiler breast meat.Crossref | GoogleScholarGoogle Scholar |

Maia EL, Rodriguez-Amaya DB (1993) Avaliação de um método simples e econômico para metilação de ácidos graxos de lipídeos de diversas espécies de peixes. Revista do Instituto Adolfo Lutz 53, 27–35.

Marino R, Albenzio M, Della Malva A, Caroprese M, Santillo A, Sevi A (2014) Changes in meat quality traits and sarcoplasmic proteins during aging in three different cattle breeds. Meat Science 98, 178–186.
Changes in meat quality traits and sarcoplasmic proteins during aging in three different cattle breeds.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2cXhtFKjsr3O&md5=3272b3e4300c46127a94332b55c1536aCAS |

Monahan FJ, Skibsted LH, Andersen ML (2005) Mechanism of oxymyoglobin oxidation in the presence of oxidizing lipids in bovine muscle. Journal of Agricultural and Food Chemistry 53, 5734–5738.
Mechanism of oxymyoglobin oxidation in the presence of oxidizing lipids in bovine muscle.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXltlKjtbg%3D&md5=aeaf93363db11951acf310e3e2f0ad02CAS |

Morgan JB, Wheeler TL, Koohmaraie M, Savell JW, Crouse JD (1993) Meat tenderness and the calpain proteolytic system in Longissimus muscle of young bulls and steers. Journal of Animal Science 71, 1471–1476.

Moura JWF, Medeiros FM, Alves MGM, Batista ASM (2015) Fatores influenciadores na qualidade da carne suína. Revista Científica de Produção Animal 17, 18–29.
Fatores influenciadores na qualidade da carne suína.Crossref | GoogleScholarGoogle Scholar |

Nishimura T (2015) Role of extracellular matrix in development of skeletal muscle and postmortem aging of meat. Meat Science 109, 48–55.
Role of extracellular matrix in development of skeletal muscle and postmortem aging of meat.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2MXhtFajs7fK&md5=c354813184cbb0e182e831d3e9d621dfCAS |

Nkukwana TT, Muchenje V, Masika PJ, Hoffman LC, Dzama K, Descalzo AM (2014) Fatty acid composition and oxidative stability of breast meat from broiler chickens supplemented with Moringa oleifera leaf meal over a period of refrigeration. Food Chemistry 142, 255–261.
Fatty acid composition and oxidative stability of breast meat from broiler chickens supplemented with Moringa oleifera leaf meal over a period of refrigeration.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXhtlGlsL7L&md5=59d0ec0fb21c1416cc9dc27e75f7e296CAS |

Oliveira FR, Boari CA, Pires AV, Mognato JC, Carvalho RMS, Santos MA, Mattioli CC (2015) Pre slaughter fasting and free-range broilers meat quality. Revista Brasileira de Saúde e Produção Animal 16, 667–677.
Pre slaughter fasting and free-range broilers meat quality.Crossref | GoogleScholarGoogle Scholar |

Pearce KL, Rosenvold K, Andersen HJ, Hopkins DL (2011) Water distribution and mobility in meat during the conversion of muscle to meat and ageing and the impacts on fresh meat quality attributes – a review. Meat Science 89, 111–124.
Water distribution and mobility in meat during the conversion of muscle to meat and ageing and the impacts on fresh meat quality attributes – a review.Crossref | GoogleScholarGoogle Scholar |

Pérez-Vendrell AM, Hernández M, Llauradó L, Schierle J, Brufau J (2001) Influence of source and ratio of xantophyll pigments on broiler chicken pigmentation and performance. Poultry Science 80, 320–326.
Influence of source and ratio of xantophyll pigments on broiler chicken pigmentation and performance.Crossref | GoogleScholarGoogle Scholar |

Santos HC, Brandelli A, Ayub MAZ (2004) Influence of post-mortem aging in tenderness of chicken breast fillets. Ciência Rural 34, 905–910.
Influence of post-mortem aging in tenderness of chicken breast fillets.Crossref | GoogleScholarGoogle Scholar |

SAS Institute Inc. (2002–2003) ‘SAS version 9.1.’ (SAS Institute Inc.: Cary, NC)

Sirri F, Castellini C, Bianchi M, Petracci M, Meluzzi A, Franchini A (2011) Effect of fast-, medium- and slow-growing strains on meat quality of chickens reared under the organic farming method. Animal 5, 312–319.
Effect of fast-, medium- and slow-growing strains on meat quality of chickens reared under the organic farming method.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BC38vovFagug%3D%3D&md5=9760c86dee5c65a199d815ba20661f6aCAS |

Taylor RG, Geesink GH, Thompson VF, Koohmaraie M, Goll DE (1995) Is Z-disk degradation responsible for postmortem tenderization? Journal of Animal Science 73, 1351–1367.
Is Z-disk degradation responsible for postmortem tenderization?Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2MXlsFSjtb0%3D&md5=add3dc4e6e48bdde56cd06b397592469CAS |

Vyncke W (1970) Direct determination of the thiobarbituric acid value in trichloracetic acid extracts of fish as a measure of oxidative rancidity. Fette, Seifen, Anstrichmittel 72, 1084–1087.
Direct determination of the thiobarbituric acid value in trichloracetic acid extracts of fish as a measure of oxidative rancidity.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaE3MXnsFantg%3D%3D&md5=abd4407e9b4723da1e28ff78ae5bf7bdCAS |

Wang KH, Shi SR, Dou TC, Sun HJ (2009) Effect of a free-range raising system on growth performance, carcass yield, and meat quality of slow-growing chicken. Poultry Science 88, 2219–2223.
Effect of a free-range raising system on growth performance, carcass yield, and meat quality of slow-growing chicken.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD1Mnjt1Sruw%3D%3D&md5=7236ec640155a37179ed3df10aa4d8f4CAS |

Watanabe A, Daly CC, Devine CE (1996) The effects of the ultimate pH of meat on tenderness changes during ageing. Meat Science 42, 67–78.
The effects of the ultimate pH of meat on tenderness changes during ageing.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BC3Mbntlelsg%3D%3D&md5=85ae0daa4bc1696ab3a599aed472b907CAS |

Wijendran V, Hayes KC (2004) Dietary n-6 and n-3 fatty acid balance and cardiovascular health. Annual Review of Nutrition 24, 597–615.
Dietary n-6 and n-3 fatty acid balance and cardiovascular health.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2cXlvFKksLg%3D&md5=6433e929875b9c4a1b11440821a27f4dCAS |

Wood JD, Enser M, Fisher AV, Nute GR, Sheard PR, Richardson RI, Hughes SI, Whittington FM (2008) Fat deposition, fatty acid composition and meat quality: a review. Meat Science 78, 343–358.
Fat deposition, fatty acid composition and meat quality: a review.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXhsVCls7s%3D&md5=5087ec4dbce501875a943f79ddaa74f8CAS |