Chemical composition and production of ethanol and other volatile organic compounds in sugarcane silage treated with chemical and microbial additives
Lucas Ladeira Cardoso A , Karina Guimarães Ribeiro A C , Marcos Inácio Marcondes A , Odilon Gomes Pereira A and Kirsten Weiβ BA Department of Animal Science, Universidade Federal de Viçosa, 36570-900, Viçosa, Brazil.
B Humboldt Universität zu Berlin, 10115, Berlin, Germany.
C Corresponding author. Email: karinaribeiro@ufv.br
Animal Production Science 59(4) 721-728 https://doi.org/10.1071/AN16204
Submitted: 3 April 2016 Accepted: 12 February 2018 Published: 17 May 2018
Abstract
Sugarcane silage can be used in animal production systems; however, it is important to apply additives to improve its chemical composition and fermentative quality. We evaluated the effect of chemical (urea and calcium oxide (CaO)) and microbial (Lactobacillus buchneri (LB), Lactobacillus plantarum, Pediococcus pentosaceus, and Propionibacterium acidipropionici) additives on chemical composition, fermentation profile, microorganism population, and production of ethanol and other volatile organic compounds in sugarcane silage. Treatments studied were silage without inoculant (SS), SS with LB, SS with Lactobacillus plantarum and Pediococcus pentosaceus, SS with Lactobacillus plantarum and Propionibacterium acidipropionici, SS with 5 g CaO/kg fresh material (FM) (5CaO), SS with 10 g CaO/kg FM (10CaO), SS with 5 g urea/kg FM (5urea), and SS with 10 g urea/kg FM (10urea). The highest crude protein content (P = 0.001) and the lowest N-linked to fibre content (P = 0.001) occurred when applying urea. None of the treatments reduced the presence of yeast (P = 0.054), but a trend was detected of treatments based on CaO as promising in this Control. The silages treated with CaO had lower ethyl ester and ethanol (average for CaO-based treatments of 0.012 g/kg dry matter and 0.695 g/kg dry matter, respectively), and silages treated with 10urea had less acetone (P = 0.001) and methanol (P = 0.001). The sugarcane silages treated with chemical additive CaO reduced ethanol production and ester formation. There was a high correlation (r = 0.984) between ethyl acetate + ethyl lactate and ethanol contents.
Additional keywords: calcium oxide, esters, Lactobacillus buchneri, organic acid, urea.
References
Alli I, Fairbairn R, Baker BE (1983) The effects of ammonia on the fermentation of chopped sugarcane. Animal Feed Science and Technology 9, 291–299.| The effects of ammonia on the fermentation of chopped sugarcane.Crossref | GoogleScholarGoogle Scholar |
AOAC (1990) ‘Official methods of analysis.’ 15th edn. (Association of Official Analytical Chemists: Washington, DC)
Ávila CLS, Carvalho BF, Pinto JC, Duarte WF, Schwan RF (2014) The use of Lactobacillus species as starter cultures for enhancing the quality of sugarcane silage. Journal of Dairy Science 97, 940–951.
| The use of Lactobacillus species as starter cultures for enhancing the quality of sugarcane silage.Crossref | GoogleScholarGoogle Scholar |
Balieiro Neto G, Siqueira GR, Reis RA, Nogueira JR, Roth MTP, Roth APTP (2007) Óxido de cálcio como aditivo na ensilagem de cana-de-açúcar. Revista Brasileira de Zootecnia 36, 1231–1239.
| Óxido de cálcio como aditivo na ensilagem de cana-de-açúcar.Crossref | GoogleScholarGoogle Scholar |
Bernardes TF, Reis RA, Amaral RC (2009) Chemical and microbiological changes and aerobic stability of Marandu grass silages after silo opening. Revista Brasileira de Zootecnia 38, 1–8.
| Chemical and microbiological changes and aerobic stability of Marandu grass silages after silo opening.Crossref | GoogleScholarGoogle Scholar |
Carvalho GGP, Pires AJV, Veloso CM, Magalhães AF, Freire MAL, Silva FF, Silva RR, Carvalho BMA (2006) Valor nutritivo do bagaço de cana-de-açúcar amonizado com quatro doses de ureia. Pesquisa Agropecuária Brasileira 41, 125–132.
| Valor nutritivo do bagaço de cana-de-açúcar amonizado com quatro doses de ureia.Crossref | GoogleScholarGoogle Scholar |
Cavali J, Pereira OG, Valadares Filho SC, Carvalho GGP, Santos MV, Porto MO, Rodrigues JFH (2010) Bromatological and microbiological characteristics of sugarcane silages treated with calcium oxide. Revista Brasileira de Zootecnia 39, 1398–1408.
| Bromatological and microbiological characteristics of sugarcane silages treated with calcium oxide.Crossref | GoogleScholarGoogle Scholar |
Custódio L, Morais G, Daniel JLP, Pauly T, Nussio LG (2016) Effects of chemical and microbial additives on clostridium development in sugarcane (Saccharum officinarum L.) ensiled with lime. Grassland Science 62, 135–143.
| Effects of chemical and microbial additives on clostridium development in sugarcane (Saccharum officinarum L.) ensiled with lime.Crossref | GoogleScholarGoogle Scholar |
Dias AM, Ítavo LCV, Ítavo CCBF, Blan LR, Gomes ENO, Soares CM, Leal ES, Nogueira E, Coelho EM (2014) Ureia e glicerina bruta como aditivos na ensilagem de cana-de-açúcar. Arquivo Brasileiro de Medicina Veterinária e Zootecnia 66, 1874–1882.
| Ureia e glicerina bruta como aditivos na ensilagem de cana-de-açúcar.Crossref | GoogleScholarGoogle Scholar |
Fortaleza APS, Silva LDF, Zackm E, Barbero RP, Ribeiro ELA, Pergoraro M, Santos LE, Mizubuti IY (2012) Composição química e degradabilidade ruminal de silagens da cana-de-açúcar tratada com aditivos químicos e bacteriano. Semina 33, 3341–3352.
Gentil RS, Pires AV, Susin I, Nussio LG, Mendes CQ, Mourão GB (2007) Digestibilidade aparente de dietas contendo silagem de cana-de-açúcar tratada com aditivo químico ou microbiano para cordeiros. Acta Scientiarum 29, 63–69.
Gerlach K, Ross F, Weiss K, Büscher W, Südekum KH (2013) Changes in maize silage fermentation products during aerobic deterioration and effects on dry matter intake by goats. Agricultural and Food Science 22, 168–181.
Jobim CC, Nussio LG, Reis RS, Schmidt P (2007) Avanços metodológicos na avaliação da qualidade da forragem conservada. Revista Brasileira de Zootecnia 36, 101–119.
| Avanços metodológicos na avaliação da qualidade da forragem conservada.Crossref | GoogleScholarGoogle Scholar |
Kononoff PJ, Heinrichs AJ, Buckmaster AD (2003) Modification of the Penn State forage and TMR separator and the effects of moisture content on its measurements. Journal of Dairy Science 86, 1858–1863.
| Modification of the Penn State forage and TMR separator and the effects of moisture content on its measurements.Crossref | GoogleScholarGoogle Scholar |
Kung L, Shaver R (2001) Interpretation and use of silage fermentation analysis reports. Focus on Forage 3, 1–5.
Kung L, Stokes M, Lin CJ (2003) Silage additives. Agronomy (Basel) 42, 305–360.
Licitra G, Hernandez TM, Van Soest PJ (1996) Standardization of procedures for nitrogen fractionation of ruminant feeds. Animal Feed Science and Technology 57, 347–358.
| Standardization of procedures for nitrogen fractionation of ruminant feeds.Crossref | GoogleScholarGoogle Scholar |
Mahanna B (1994) Proper management assures high-quality silage, grains. Feedstuffs 10, 12–18.
McDonald P, Henderson AR, Heron SJE (1991) ‘The biochemistry of silage.’ (Chalcombe Publications: Marlow Bottom, Buckinghamshire, UK)
Mertens DR (2002) Gravimetric determination of amylase treated neutral detergent fiber in feeds with refluxing in beakers or crucibles: collaborative study. Journal of AOAC International 85, 1217–1240.
Moraes KAK, Valadares Filho SC, Moraes EHBK, Pina DS (2015) Calcium oxide levels in sugarcane silage, fresh sugarcane or corn silage for feedlot nellore heifers. The Journal of Animal and Plant Sciences 25, 989–996.
Okuda H, Fujii S, Kawashima Y (1965) A direct colorimetric determination of blood ammonia. The Tokushima Journal of Experimental Medicine 12, 11–23.
Oude Elferink SJWH, Diehuis F, Krooneman J, Gottschal JC, Spoelstra SF (1999) Lactobacillus buchneri can improve the aerobic stability of silage via a novel fermentation pathway: the anaerobic degradation of lactic acid to acetic acid and 1,2 propanediol. In ‘Proceedings XII international silage conference’. pp. 266–267. (Ultuna, Uppsala, Sweden)
Oude Elferink SJWH, Krooneman J, Gottschal JC, Spoelstra SF, Faber F, Driehuis F (2001) Anaerobic conversion of lactic acid to acetic acid and 1.2-propanediol by Lactobacillus buchneri. Applied and Environmental Microbiology 67, 125–132.
| Anaerobic conversion of lactic acid to acetic acid and 1.2-propanediol by Lactobacillus buchneri.Crossref | GoogleScholarGoogle Scholar |
Pedroso AF, Nussio LG, Loures DRS, Paziani SF, Igarasi MS, Coelho RM, Horii J, Rodrigues AA (2007) Efeito do tratamento com aditivos químicos e inoculantes bacterianos nas perdas e na qualidade de silagens de cana-de-açúcar. Revista Brasileira de Zootecnia 36, 558–564.
| Efeito do tratamento com aditivos químicos e inoculantes bacterianos nas perdas e na qualidade de silagens de cana-de-açúcar.Crossref | GoogleScholarGoogle Scholar |
Playne MJ, McDonald P (1966) The buffering constituents of herbage and of silage. Journal of the Science of Food and Agriculture 17, 264–268.
| The buffering constituents of herbage and of silage.Crossref | GoogleScholarGoogle Scholar |
Rabelo CHS, Costa AP, Rezende AV, Harter CJ, Florentino LA, Rabelo FHS (2014) What is the best additive to use at the ensiling of sugarcane SP81–3250? Animal Production Science 54, 1682–1686.
| What is the best additive to use at the ensiling of sugarcane SP81–3250?Crossref | GoogleScholarGoogle Scholar |
Rooke JA, Hatfield RD (2003) Biochemistry of ensiling. Agronomy (Basel) 42, 95–139.
Santos MC, Nussio LG, Mourão GB, Schmidt P, Mari LJE, Ribeiro JL (2008) Influência da utilização de aditivos químicos no perfil da fermentação, no valor nutritivo e nas perdas de silagens de cana-de-açúcar. Revista Brasileira de Zootecnia 37, 1555–1563.
| Influência da utilização de aditivos químicos no perfil da fermentação, no valor nutritivo e nas perdas de silagens de cana-de-açúcar.Crossref | GoogleScholarGoogle Scholar |
Santos WCC, Nascimento ALR, Silva DKA, Silva WJCS, Santana AVS, Soares GSC (2015) Nutritive value, total losses of dry matter and aerobic stability of the silage from three varieties of sugarcane treated with commercial microbial additives. Animal Feed Science and Technology 204, 1–8.
| Nutritive value, total losses of dry matter and aerobic stability of the silage from three varieties of sugarcane treated with commercial microbial additives.Crossref | GoogleScholarGoogle Scholar |
SAS (2008) ‘SAS/STAT user’s guide.’ (SAS Institute: Cary, NC)
Schmidt P, Nussio LG, Queiroz OCM, Santos MC, Zopollatto M, Toledo Filho SG, Daniel JLP (2014) Effects of Lactobacillus buchneri on the nutritive value of sugarcane silage for finishing beef bulls. Revista Brasileira de Zootecnia 43, 8–13.
| Effects of Lactobacillus buchneri on the nutritive value of sugarcane silage for finishing beef bulls.Crossref | GoogleScholarGoogle Scholar |
Siqueira GR, Roth MTP, Moretti MH, Benatti JMB, Resende FD (2012) Uso da cana-de-açúcar na alimentação de ruminantes. Revista Brasileira de Saúde e Produção Animal 13, 991–1008.
| Uso da cana-de-açúcar na alimentação de ruminantes.Crossref | GoogleScholarGoogle Scholar |
Tomich TR, Pereira LGR, Gonçalves LC, Tomich RGP, Borges I (2003) ‘Características químicas para avaliação do processo fermentativo de silagens: uma proposta para qualificação da fermentação.’ Documento 57. (Embrapa Pantanal: Corumbá, MS, Brazil)
Van Soest PJ, Robertson JB (Ed.) (1985) ‘Analysis of forages and fibrous foods.’ (Cornell University Press: Ithaca, NY)
Weiß K, Auerbach H (2012) Occurrence of volatile organic compounds and ethanol in different types of silages. In ‘Proceedings of the XVI international silage conference MTT Agrifood Research Finland’. (Eds K Kuoppala, M Rinne, A Vanhatalo) pp. 128–129. (University of Helsinki: Hämeenlinna, Finland)
Weiß K, Kaiser E (1995) Milchsäurebestimmung in Silageextrakten mit Hilfe der HPLC. Das wirtschaftseigene Futter 41, 69–80.
Weiß K, Sommer G (2012) Bestimmung von Estern und anderen flüchtigen organischen Substanzen (VOC) in Silageextrakten mit Hilfe der Gaschromatographie. VDLUFA-Schriftenreihe 68, 561–569.
Weiß K, Kalzendorf C, Zittlau J, Auerbach H (2009) Novel results on the occurrence of volatile compounds in maize silages. In ‘Proceedings of the XVth international silage conference’. (Eds GA Broderick, AT Adesogan, LW Bocher, KK Bolsen, FE Contreras-Govea, JH Harrison, RE Muck) pp. 33–34. (Madison, Wisconsin, USA)
Weißbach F (2009) Correction of dry matter content of silages used as substrate for biogas production. In ‘Proceedings of XV international silage conference’. (Eds GA Broderick, AT Adesogan, LW Bocher, KK Bolsen, FE Contreras-Govea, JH Harrison, RE Muck) pp. 483–484. (Madison, Wisconsin, USA)
Woolford MK (1984) ‘The silage fermentation.’ (Marcel Dekker: New York)