Single nucleotide polymorphisms associated with osteochondrosis dissecans in Warmblood horses at different stages of training
D. Lewczuk A E , M. Hecold B , A. Ruść C , M. Frąszczak D , A. Bereznowski B , A. Korwin-Kossakowska A , S. Kamiński C and J. Szyda D
+ Author Affiliations
- Author Affiliations
A Institute of Genetics and Animal Breeding PAS, ul.Postępu 36A, 05-552 Magdalenka, Poland.
B Warsaw University of Life Science-SGGW, ul.Nowoursynowska 166, 02-787 Warsaw, Poland.
C University of Warmia and Mazury, ul.Oczapowskiego 5, 10-719 Olsztyn, Poland.
D University of Environmental and Life Science, ul.CK Norwida25/27, 50-375 Wrocław, Poland.
E Corresponding author. Email: d.lewczuk@ighz.pl
Animal Production Science 57(4) 608-613 https://doi.org/10.1071/AN15450
Submitted: 13 August 2015 Accepted: 14 January 2017 Published: 2 March 2017
Journal Compilation © CSIRO Publishing 2017 Open Access CC BY-NC-ND
Abstract
The genetic background of osteochondrosis dissecans (OCD) has been studied for years, but the compatibility of the position of markers has not been reached between results, probably because of unknown additional effects that may influence the results, such as definition of the trait, gene–environmental interactions and the dynamics of trait development. The aim of the study was to identify single nucleotide polymorphisms (SNP) associated with the occurrence of OCD in Polish Warmblood sport breed horses in two different stages of training. Warmblood horses (87 stallions and 114 mares) were phenotyped and genotyped. Horses were X-rayed twice, at the beginning and at the end of the tests (100 days for stallions and 60 days for mares). Ten images per horse were collected using digital equipment for the fetlocks, stifles and hocks. The DNA was genotyped using the Illumina Neogen Equine Array. Statistical analysis included the Cochran–Armitage test and logistic regression assuming an additive model of inheritance. The Monte Carlo Markov Chain method was also applied to determine heritability coefficients. Nineteen and twenty SNP were identified that were significantly associated with OCD using logistic regression at the first and second stage of training, respectively. Four SNP were significant for both stages of training. The estimation of the heritability of a horse’s OCD status does not achieve the same level at different stages of training. The study on the genetic background of horse OCD should include as much detailed information on their training as possible.
Additional keywords: disease, orthopeadic, selection, SNP.
References
Andersson LS, Larhammar M, Memic F, Wootz H, Schwochow D, Rubin CJ, Patra K, Arnason T, Wellbring L, Hjälm G, Imsland F, Petersen JL, McCue ME, Mickelson JR, Cothran G, Ahituv N, Roepstorff L, Mikko S, Vallstedt A, Lindgren G, Andersson L, Kullander K (2012) Mutations in DMRT3 affect locomotion in horses and spinal circuit function in mice. Nature 488, 642–646.| Mutations in DMRT3 affect locomotion in horses and spinal circuit function in mice.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38Xht1ynt7zM&md5=8fca8dbe8aaf0d51cdd6108be9571873CAS |
Bannasch D (2008) Genetic testing and the future of equine genomics. Journal of Equine Veterinary Science 28, 645–649.
| Genetic testing and the future of equine genomics.Crossref | GoogleScholarGoogle Scholar |
Barrey E (2010) Genetics and genomics in equine exercise physiology: an overview of the new applications of molecular biology as positive and negative markers of performance and health. Equine Veterinary Journal 42, 561–568.
| Genetics and genomics in equine exercise physiology: an overview of the new applications of molecular biology as positive and negative markers of performance and health.Crossref | GoogleScholarGoogle Scholar |
Betin VMS, Singleton BK, Parson SF, Austee DJ, Lane JD (2013) Autophagy facilitates organelle clearance during differentiation of human erythoblasts. Autophagy 9, 881–893.
| Autophagy facilitates organelle clearance during differentiation of human erythoblasts.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXhvVOnsr%2FN&md5=dd20fe16feb0d7c4f8acbea1abaf398fCAS |
Brosnahan MM, Brooks SA, Antczak DF (2010) Equine clinical genomics: A clinician’s primer. Equine Veterinary Journal 42, 658–670.
| Equine clinical genomics: A clinician’s primer.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BC3cfisFyisQ%3D%3D&md5=2ed9159d49713d69e8ed4f691fe46746CAS |
Castle K (2012) The investigating the genetic and genomic basis of osteochondrosis in Thoroughbred horses from Australia and New Zealand. PhD Thesis, The University of Sydney, Sydney, Australia. Available at http://ses.library.usyd.edu.au/handle/2123/9369 [Verified 16 February 2017]
Corbin LJ, Blott SC, Swinburne JE, Vaudin M, Bishop SC, Woolliams JA (2010) Linkage disequilibrium and historical effective population size in the Thoroughbred horse. Animal Genetics 41, 8–15.
| Linkage disequilibrium and historical effective population size in the Thoroughbred horse.Crossref | GoogleScholarGoogle Scholar |
Corbin LJ, Blott SC, Swinburne JE, Sibbons C, Fox-Clipsham LY, Helwegen M, Parkin TDH, Newton JR, Bramlage LR, McIlwaith CW, Bishop SC, Woolliams JA, Vaudin M (2012) A genome-wide association study of osteochondritis dissecans in the Thoroughbred. Mammalian Genome 23, 294–303.
| A genome-wide association study of osteochondritis dissecans in the Thoroughbred.Crossref | GoogleScholarGoogle Scholar |
Corbin LJ, Kranis S, Blott SC, Swinburne JE, Vaudin M, Bishop SC, Woolliams JA (2014) The utility of low-density genotyping for imputation in the Thoroughbred horse. Genetics, Selection, Evolution. 46, 9
| The utility of low-density genotyping for imputation in the Thoroughbred horse.Crossref | GoogleScholarGoogle Scholar |
Denoix J.-M., Jeffcott L.B., McIlwraith C.W., van Weeren P.R. (2013) A review of terminology for equine juvenile osteochondral conditions (JOCC) based on anatomical and functional considerations. Veterinary Journal (London, England) 197, 29–35.
| A review of terminology for equine juvenile osteochondral conditions (JOCC) based on anatomical and functional considerations.Crossref | GoogleScholarGoogle Scholar |
Distl O (2013) The genetics of equine osteochondrosis. Veterinary Journal (London, England) 197, 13–18.
| The genetics of equine osteochondrosis.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXhtVOjurfO&md5=53d01db59911fecf7ed36a59964ff1d6CAS |
Falconer DS, Mackay TFC (1996) Threshold characters. In ‘Introduction to quantitative genetics’. (Eds DS Falconer, TFC Mackay) pp. 299–311. (Pearson: Harlow, UK)
Finno CJ, Bannasch DL (2014) Applied equine genetics. Equine Veterinary Journal 46, 538–544.
| Applied equine genetics.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BC2cjgt1eqsA%3D%3D&md5=e8d4b601c17c7f864d25fe330d409c32CAS |
Gallagher PC, Morrison S, Bernoco D, Bailey E (2003) Measurement of back curvature in American Saddlebred horses: structural and genetic basis for early-onset lordosis. Journal of Equine Veterinary Science 23, 71–76.
| Measurement of back curvature in American Saddlebred horses: structural and genetic basis for early-onset lordosis.Crossref | GoogleScholarGoogle Scholar |
Hadfield J (2010) MCMC methods for multi-response GLMM: the MCMCglmm R package. Journal of Statistical Software 33, 1–22.
| MCMC methods for multi-response GLMM: the MCMCglmm R package.Crossref | GoogleScholarGoogle Scholar |
Halper J, Kim B, Khan A, Yoon JH, Mueller POE (2006) Degenerative suspensory ligament desmitis as a systemic disorder characterized by proteoglycan accumulation. BMC Veterinary Research 2,
| Degenerative suspensory ligament desmitis as a systemic disorder characterized by proteoglycan accumulation.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XksVSqu7k%3D&md5=276ab273a2af64215c4a7ca72c2763c7CAS |
Hikiji H, Eudo D, Horie K, Harayama T, Akahoshi N, Igarashi H, Kihara Y, Yanagida K, Takeda J, Takehiko K, Shimizu T, Ishii S (2014) TDA8 activation inhibits osteoclastic bone resorption. The FASEB Journal 28, 871–879.
| TDA8 activation inhibits osteoclastic bone resorption.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2cXisVGrurc%3D&md5=7f450a6959edb942093419c9d98f8f4aCAS |
Kawai T, Nomura F, Hoshino K, Copeland NG, Gilbert DJ, Jenkins NA, Akira S (1999) Death-associated protein kinase 2 is a new calcium/calmodulin-dependent protein kinase that signals apoptosis through its catalytic activity. Oncogene 18, 3471–3480.
Lampe V, Komm K, Lichtner P, Meitinger T, Distl O (2010) Genome wide association analysis for osteochondrosis in Hanoverian warmblood horses using a SNP assay. Chapter 5. In PhD Thesis Komm K: Fine mapping of quantitative trait loci (QTL) for osteochondrosis in Hanoverian warmblood horses. University of Veterinary Medicine Hannover, Hannover, Germany.
Lewczuk D, Korwin-Kossakowska A (2013) Genetic background of osteochondrosis in horses. Animal Science Papers and Reports 30, 205–218.
Lykkjen S, Dolvik NI, McCue ME, Rendahl AK, Mickelson JR, Røed KH (2010) Genome-wide association analysis of osteochondrosis of the tibiotarsal joint in Norwegian Standardbred trotters. Animal Genetics 41, 111–120.
| Genome-wide association analysis of osteochondrosis of the tibiotarsal joint in Norwegian Standardbred trotters.Crossref | GoogleScholarGoogle Scholar |
Lykkjen S, Dolvik NI, McCue ME, Rendahl AK, Mickelson JR, Røed KH (2013) Equine developmental orthopaedic diseases - a genome-wide association study of first phalanx plantar osteochondral fragments in Standardbred trotters. Animal Genetics 44, 766–769.
| Equine developmental orthopaedic diseases - a genome-wide association study of first phalanx plantar osteochondral fragments in Standardbred trotters.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXhs12mtbvP&md5=3bf4452fff91b2ad6be9d02cca5fcd7fCAS |
O’Shea PJ, Kim DW, Logan JG, Davis S, Walker RL, Meltzer PS, Cheng SY, Williams GR (2012) Advanced bone formation in mice with a dominant-negative mutation in the thyroid hormone receptor β gene due to activation of Wnt/β - catenin protein signaling. The Journal of Biological Chemistry 287, 17812–17822.
| Advanced bone formation in mice with a dominant-negative mutation in the thyroid hormone receptor β gene due to activation of Wnt/β - catenin protein signaling.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38XntFSms7c%3D&md5=136c155747bdcacc555353b5f139e56fCAS |
Okuda T, Hirai H, Valentine VA, Shurtleff SA, Kidd VJ, Lahti JM, Sherr CJ, Downing JR (1995) Molecular cloning, expression pattern, and chromosomal localization of human CDKN2D/INK4d, an inhibitor of cyclin D-dependent kinases. Genomics 29, 623–630.
Oki H, Miyake T, Kasashima Y, Sasaki Y (2008) Estimation of heritability for superficial digital flexor tendon injury by Gibbs sampling in the Thoroughbred racehorse. Journal of Animal Breeding and Genetics 125, 413–416.
| Estimation of heritability for superficial digital flexor tendon injury by Gibbs sampling in the Thoroughbred racehorse.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD1M%2FmtlOqtg%3D%3D&md5=7872d9a05bab19481555445c9920121eCAS |
Orr N, Hill EW, Gu J, Goviandarajan P, Conroy J, van Grevenhof EM, Ducro BJ, Arendonk JAM, Knaap JH, van Weeren PR, MacHugh DE, Ennis S, Brama PAJ (2013) Genome-wide association study of osteochondrosis in the tarsacrural joint of Dutch Warmblood horses identifies susceptibility loci on chromosome 3 and 10. Animal Genetics 44, 408–412.
| Genome-wide association study of osteochondrosis in the tarsacrural joint of Dutch Warmblood horses identifies susceptibility loci on chromosome 3 and 10.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXhtVGksLbK&md5=313c85e99f9f03267247b615fae876a2CAS |
Teyssèdre S, Dupuis MC, Elsen JM, Guérin G, Schibler L, Denoix JM, Ricard A (2010) Genome-wide SNP association study identifies region of interest associated with osteochondrosis in French Trotters. In Proceedings of the 9th world congress on genetics applied to livestock production, Leipzig, Germany, 1–6 August 2010. Available at http://www.kongressband.de/wcgalp2010/ [Verified 12 August 2015]
Teyssèdre S, Dupuis MC, Guérin G, Schibler L, Denoix JM, Elsen JM, Ricard A (2012) Genome-wide SNP association studies for osteochondrosis in French Trotters. Journal of Animal Science 90, 45–53.
| Genome-wide SNP association studies for osteochondrosis in French Trotters.Crossref | GoogleScholarGoogle Scholar |
van Weeren PR (2006) Etiology, diagnosis and treatment of OC(D). Clinical Techniques in Equine Practice 5, 248–258.
| Etiology, diagnosis and treatment of OC(D).Crossref | GoogleScholarGoogle Scholar |
Villemereuil P (2012) Estimation of a biological trait heritability using the animal model. Available at devillemereuil.legtux.org/wp-content/uploads/2012/12/tuto_en.pdf [Verified 12 August 2015]
