Comparison of rumen in vitro fermentation of temperate pastures using different batch culture systems
Juan Pablo Keim A D , Robert Berthiaume B , David Pacheco C and Stefan Muetzel C
+ Author Affiliations
- Author Affiliations
A Animal Production Institute, Faculty of Agricultural Sciences, Universidad Austral de Chile, PO Box 567, Valdivia, Chile.
B Valacta, Dairy Centre of Expertise, 555 Blvd des anciens-combattants, Ste-Anne-de-Bellevue, QC H9X 3R4, Canada.
C Animal Science Group, Grasslands Research Centre, AgResearch Limited, Private Bag 11008, Palmerston North, New Zealand.
D Corresponding author. Email: juan.keim@uach.cl
Animal Production Science 57(4) 690-696 https://doi.org/10.1071/AN15190
Submitted: 15 April 2015 Accepted: 12 January 2016 Published: 3 May 2016
Abstract
In vitro batch culture systems are popular because they are relatively inexpensive and allow the screening and testing of large amounts of samples in a short time. Most of the batch culture systems have been designed for the evaluation of gas produced during fermentation of substrates and different designs have been compared between laboratories, but very little work is published where methane production or volatile fatty acid production is compared. The aim of this study was to determine the degree of agreement between two different in vitro batch culture systems, from different laboratories when measuring in vitro fermentation kinetics and end products using pasture samples as substrates. The two systems were a manual and a fully automated pressure-based system. Duplicates of pasture samples were incubated in three consecutive runs. Concordance correlation coefficients between systems and estimates of variance components (pasture, incubation run and random error) for each system were determined for all measured variables. There were poor correlations between systems for most of the variables except for time to produce half of the asymptotic gas production and acetate molar proportion of volatile fatty acids. However, for both systems most of variance was due to pasture sample and then incubation run. The poor agreement between systems might be explained by the different laboratory protocols. Therefore, comparisons of absolute values from different batch culture systems or experiments must be done carefully. Alternatively, more standardisation in terms of sample preparation and incubation procedure may be needed to compare in vitro fermentation products among systems.
Additional keywords: gas production, methane, volatile fatty acids.
References
Attwood GT, Klieve AV, Ouwerkerk D, Patel BKC (1998) Ammonia-hyperproducing bacteria from New Zealand ruminants. Applied and Environmental Microbiology 64, 1796–1804.Benchaar C, Greathead H (2011) Essential oils and opportunities to mitigate enteric methane emissions from ruminants. Animal Feed Science and Technology 166–167, 338–355.
| Essential oils and opportunities to mitigate enteric methane emissions from ruminants.Crossref | GoogleScholarGoogle Scholar |
Blakeney AB, Mutton LL (1980) A simple colorimetric method for the determination of sugar in fruit and vegetable. Journal of the Science of Food and Agriculture 31, 889–897.
| A simple colorimetric method for the determination of sugar in fruit and vegetable.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL3MXhtVWnsb8%3D&md5=92259cd32bbb5df36b98ce67948cc179CAS |
Blümmel M, Steingass H, Becker K (1997) The relationship, between in vitro gas production, in vitro microbial biomass yield and 15Nincorporation and its implications for the prediction of voluntary feed intake of roughages. British Journal of Nutrition 77, 911–921.
| The relationship, between in vitro gas production, in vitro microbial biomass yield and 15Nincorporation and its implications for the prediction of voluntary feed intake of roughages.Crossref | GoogleScholarGoogle Scholar | 9227188PubMed |
Boguhn J, Zuber T, Rodehutscord M (2013) Effect of donor animals and their diet on in vitro nutrient degradation and microbial protein synthesis using grass and corn silages. Journal of Animal Physiology and Animal Nutrition 97, 547–557.
| Effect of donor animals and their diet on in vitro nutrient degradation and microbial protein synthesis using grass and corn silages.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXhtVGiu7vN&md5=3fb17200c7a8893da2d3e51636a7641cCAS | 22487195PubMed |
Cattani M, Tagliapietra F, Maccarana L, Hansen HH, Bailoni L, Schiavon S (2014) Technical note: In vitro total gas and methane production measurements from closed or vented rumen batch culture systems. Journal of Dairy Science 97, 1736–1741.
| Technical note: In vitro total gas and methane production measurements from closed or vented rumen batch culture systems.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2cXktFKhtw%3D%3D&md5=4acab78a86b849c54f9bd7066e2254c7CAS | 24393177PubMed |
Cone JW, Van Gelder AH, Visscher GJW, Oudshoorn L (1996) Influence of rumen fluid and substrate concentration on fermentation kinetics measured with a fully automated time related gas production apparatus. Animal Feed Science and Technology 61, 113–128.
| Influence of rumen fluid and substrate concentration on fermentation kinetics measured with a fully automated time related gas production apparatus.Crossref | GoogleScholarGoogle Scholar |
Cornou C, Storm IMLD, Hindrichsen IK, Worgan H, Bakewell E, Ruiz DRY, Abecia L, Tagliapietra F, Cattani M, Ritz C, Hansen HH (2013) A ring test of a wireless in vitro gas production system. Animal Production Science 53, 585–592.
| A ring test of a wireless in vitro gas production system.Crossref | GoogleScholarGoogle Scholar |
Davies ZS, Mason D, Brooks AE, Griffith GW, Merry RJ, Theodorou MK (2000) An automated system for measuring gas production from forages inoculated with rumen fluid and its use in determining the effect of enzymes on grass silage. Animal Feed Science and Technology 83, 205–221.
| An automated system for measuring gas production from forages inoculated with rumen fluid and its use in determining the effect of enzymes on grass silage.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3cXhslentbY%3D&md5=cd1f80fcb784c56750e3a95d9ca0e957CAS |
Dijkstra J, Kebreab E, Bannink A, France J, López S (2005) Application of the gas production technique to feed evaluation systems for ruminants. Animal Feed Science and Technology 123–124, 561–578.
| Application of the gas production technique to feed evaluation systems for ruminants.Crossref | GoogleScholarGoogle Scholar |
France J, Dijkstra J, Dhanoa MS, Lopez S, Bannink A (2000) Estimating the extent of degradation of ruminant feeds from a description of their gas production profiles observed in vitro: derivation of models and other mathematical considerations. British Journal of Nutrition 83, 143–150.
| Estimating the extent of degradation of ruminant feeds from a description of their gas production profiles observed in vitro: derivation of models and other mathematical considerations.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3cXhsVymtbY%3D&md5=ea3b4276bff65a900515c6e00b16049cCAS | 10743493PubMed |
Johnson KA, Johnson DE (1995) Methane emissions from cattle. Journal of Animal Science 73, 2483–2492.
Jouany JP, Lassalas B (2002) Gas pressure inside a rumen in vitro fermentation system stimulates the use of hydrogen. Reproduction, Nutrition, Development 42, S64
Keim JP, López IF, Berthiaume R (2014) Nutritive value, in vitro fermentation and methane production of perennial pastures as affected by botanical composition over a growing season in the south of Chile. Animal Production Science 54, 598–607.
| Nutritive value, in vitro fermentation and methane production of perennial pastures as affected by botanical composition over a growing season in the south of Chile.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2cXls1ehtrs%3D&md5=9af0f13533d496265f92fc3feb3f8236CAS |
Landis JR, Koch GG (1977) The measurement of observer agreement for categorical data. Biometrics 33, 159–174.
| The measurement of observer agreement for categorical data.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DyaE2s7jsFWqtA%3D%3D&md5=e53a843e4a494a8c3db727d852d5cec9CAS | 843571PubMed |
Lin II (1989) A concordance correlation coefficient to evaluate reproducibility. Biometrics 45, 255–268.
| A concordance correlation coefficient to evaluate reproducibility.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DyaL1M3kslKrtg%3D%3D&md5=6100493ec2dcf9a849539a4ef221b80fCAS |
Lovett DK, Bortolozzo A, Conaghan P, O’Kiely P, O’Mara FP (2004) In vitro total and methane gas production as influenced by rate of nitrogen application, season of harvest and perennial ryegrass cultivar. Grass and Forage Science 59, 227–232.
| In vitro total and methane gas production as influenced by rate of nitrogen application, season of harvest and perennial ryegrass cultivar.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2cXptVensLs%3D&md5=2cce0380f264aff4fc2efa4c405b238bCAS |
Lovett DK, McGilloway D, Bortolozzo A, Hawkins M, Callan J, Flynn B, O’Mara FP (2006) In vitro fermentation patterns and methane production as influenced by cultivar and season of harvest of Lolium perenne L. Grass and Forage Science 61, 9–21.
| In vitro fermentation patterns and methane production as influenced by cultivar and season of harvest of Lolium perenne L.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XjslWhu7g%3D&md5=e9259f5b069597300c3091fc116755f7CAS |
Martínez ME, Ranilla MJ, Tejido ML, Saro C, Carro MD (2010) The effect of the diet fed to donor sheep on in vitro methane production and ruminal fermentation of diets of variable composition. Animal Feed Science and Technology 158, 126–135.
| The effect of the diet fed to donor sheep on in vitro methane production and ruminal fermentation of diets of variable composition.Crossref | GoogleScholarGoogle Scholar |
Menke KH, Steingass H (1988) Estimation of the energetic feed value obtained from chemical analyses and in vitro gas production using rumen fluid. Animal Research and Development 28, 7–55.
Mould FL, Morgan R, Kliem KE, Krystallidou E (2005) A review and simplification of the in vitro incubation medium. Animal Feed Science and Technology 123–124, 155–172.
| A review and simplification of the in vitro incubation medium.Crossref | GoogleScholarGoogle Scholar |
Muetzel S, Hunt C, Tavendale MH (2014) A fully automated incubation system for the measurement of gas production and gas composition. Animal Feed Science and Technology 196, 1–11.
| A fully automated incubation system for the measurement of gas production and gas composition.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2cXhtlamsb3I&md5=38be41c59512b6ff82ec66c2fec4e89eCAS |
Patra AK, Yu Z (2013) Effects of gas composition in headspace and bicarbonate concentrations in media on gas and methane production, degradability, and rumen fermentation using in vitro gas production techniques. Journal of Dairy Science 96, 4592–4600.
| Effects of gas composition in headspace and bicarbonate concentrations in media on gas and methane production, degradability, and rumen fermentation using in vitro gas production techniques.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXnslCgsb0%3D&md5=23002155c959521db619f0a7e5052e5fCAS | 23684023PubMed |
Pelletier S, Tremblay GF, Bertrand A, Belanger G, Castonguay Y, Michaud R (2010) Drying procedures affect non-structural carbohydrates and other nutritive value attributes in forage samples. Animal Feed Science and Technology 157, 139–150.
| Drying procedures affect non-structural carbohydrates and other nutritive value attributes in forage samples.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXkvFKrsL8%3D&md5=acf259c25a5ffb2bc51194b65e6af124CAS |
Pellikaan WF, Hendriks WH, Uwimana G, Bongers LJGM, Becker PM, Cone JW (2011) A novel method to determine simultaneously methane production during in vitro gas production using fully automated equipment. Animal Feed Science and Technology 168, 196–205.
| A novel method to determine simultaneously methane production during in vitro gas production using fully automated equipment.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXhtVKksLbK&md5=8a6929646a59aee490e9f9f94c0df0bdCAS |
Purcell PJ, O’Brien M, Navarro-Villa A, Boland TM, McEvoy M, Grogan D, O’Kiely P (2012a) In vitro rumen methane output of perennial ryegrass varieties and perennial grass species harvested throughout the growing season. Grass and Forage Science 67, 280–298.
| In vitro rumen methane output of perennial ryegrass varieties and perennial grass species harvested throughout the growing season.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38XhtVantrrF&md5=4348aaf47fe9139759ad79597eaeb3feCAS |
Purcell PJ, Grant J, Boland TM, Grogan D, O’Kiely P (2012b) The in vitro rumen methane output of perennial grass species and white clover varieties, and associative effects for their binary mixtures, evaluated using a batch-culture technique. Animal Production Science 52, 1077–1088.
| The in vitro rumen methane output of perennial grass species and white clover varieties, and associative effects for their binary mixtures, evaluated using a batch-culture technique.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38Xhs1Wju73N&md5=948e29be2f83771fcf2df3c73d16c697CAS |
Rymer C, Huntington JA, Givens DI (1999) Effects of inoculum preparation method and concentration, method of inoculation and pre-soaking the substrate on the gas production profile of high temperature dried grass. Animal Feed Science and Technology 78, 199–213.
| Effects of inoculum preparation method and concentration, method of inoculation and pre-soaking the substrate on the gas production profile of high temperature dried grass.Crossref | GoogleScholarGoogle Scholar |
Rymer C, Huntington JA, Williams BA, Givens DI (2005) In vitro cumulative gas production techniques: History, methodological considerations and challenges. Animal Feed Science and Technology 123–124, 9–30.
| In vitro cumulative gas production techniques: History, methodological considerations and challenges.Crossref | GoogleScholarGoogle Scholar |
SAS Institute (2007) ‘SAS user’s guide: basics.’ (SAS Institute Inc.: Cary, NC)
Sun XZ, Hoskin SO, Muetzel S, Molano G, Clark H (2011) Effects of forage chicory (Cichorium intybus) and perennial ryegrass (Lolium perenne) on methane emissions in vitro and from sheep. Animal Feed Science and Technology 166–167, 391–397.
| Effects of forage chicory (Cichorium intybus) and perennial ryegrass (Lolium perenne) on methane emissions in vitro and from sheep.Crossref | GoogleScholarGoogle Scholar |
Tagliapietra F, Cattani M, Bailoni L, Schiavon S (2010) In vitro rumen fermentation: effect of headspace pressure on the gas production kinetics of corn meal and meadow hay. Animal Feed Science and Technology 158, 197–201.
| In vitro rumen fermentation: effect of headspace pressure on the gas production kinetics of corn meal and meadow hay.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXmvVSrs7w%3D&md5=381a89a9e02498d1bdfffe130997b6c9CAS |
Tavendale MH, Meagher LP, Pacheco D, Walker N, Attwood GT, Subathira S (2005) Methane production from in vitro rumen incubations with Lotus pedunculatus and Medicago sativa, and effects of extractable condensed tannin fractions on methanogenesis. Animal Feed Science and Technology 123–124, 403–419.
| Methane production from in vitro rumen incubations with Lotus pedunculatus and Medicago sativa, and effects of extractable condensed tannin fractions on methanogenesis.Crossref | GoogleScholarGoogle Scholar |
Theodorou MK, Williams BA, Dhanoa MS, McAllan AB, France J (1994) A simple gas production method using a pressure transducer to determine the fermentation kinetics of ruminant feeds. Animal Feed Science and Technology 48, 185–197.
| A simple gas production method using a pressure transducer to determine the fermentation kinetics of ruminant feeds.Crossref | GoogleScholarGoogle Scholar |
Van Soest PJ, Robertson JB, Lewis BA (1991) Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science 74, 3583–3597.
| Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DyaK38%2FnvVCltA%3D%3D&md5=5ac74045d70ec4fdb1b477d24fb39c1dCAS | 1660498PubMed |
Xu M, Rinker M, McLeod KR, Harmon DL (2010) Yucca schidigera extract decreases in vitro methane production in a variety of forages and diets. Animal Feed Science and Technology 159, 18–26.
| Yucca schidigera extract decreases in vitro methane production in a variety of forages and diets.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXosVGntr0%3D&md5=6c0e8991e91680c76edc77b936f51ac7CAS |
Zhou Z, Yu Z, Meng Q (2012) Effects of nitrate on methane production, fermentation, and microbial populations in in vitro ruminal cultures. Bioresource Technology 103, 173–179.
| Effects of nitrate on methane production, fermentation, and microbial populations in in vitro ruminal cultures.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXhsFSqtrvM&md5=7632e4515f2a78c524eac5a8065c29a7CAS | 22047657PubMed |
