Methane production and in vitro digestibility of low quality forages treated with a protease or a cellulase
J. M. Cantet A C , D. Colombatto A B and G. Jaurena A
+ Author Affiliations
- Author Affiliations
A Department of Animal Production, School of Agronomy, University of Buenos Aires, Av. San Martín 4453, C1417DSE, Buenos Aires, Argentina.
B National Scientific and Technical Research Council (CONICET) of Argentina, Av. Rivadavia 1917, C1033AAJ, Buenos Aires, Argentina.
C Corresponding author. Email: jcantet@agro.uba.ar
Animal Production Science 56(10) 1700-1706 https://doi.org/10.1071/AN14988
Submitted: 5 December 2014 Accepted: 24 March 2015 Published: 16 June 2015
Abstract
The objective was to assess the impact of application of two enzyme mixtures on the in vitro dry matter digestibility, neutral detergent fibre digestibility, net cumulative gas production and methane production after 24 h of incubation of Milium coloratum (formely Panicum coloratum) and a Patagonian meadow grassland. A protease (Protex 6-L) and a fibrolytic enzyme (Rovabio) were assessed at three application rates (30, 60 and 90 mg/100 mL of distiller water) on the substrates. Meadow samples were higher to Milium ones (P < 0.05) for in vitro dry matter digestibility and net cumulative gas production at 24 h. Nevertheless, Milium was ~11% higher than meadow (P < 0.05) for methane when expressed as a proportion of digested dry matter (g/kg). Rovabio did not induce differences in any variable, but the addition of Protex reduced (P < 0.05) in vitro dry matter digestibility in both substrates without bringing about differences in methane production. Collectively, the addition of these enzymes did not benefit in vitro ruminal fermentation of low quality forages.
Additional keywords: cell wall hydrolysis, enzymes, greenhouses gases, in vitro gas production, ruminant.
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