Free Standard AU & NZ Shipping For All Book Orders Over $80!
Register      Login
Animal Production Science Animal Production Science Society
Food, fibre and pharmaceuticals from animals
RESEARCH ARTICLE

Effect of varying the energy density on growth performance, meat quality, caecum fermentation and microbiota of growing Rex rabbits

S. J. Wu A , L. Liu A , Y. L. Zhu A , C. Y. Wang A and F. C. Li A B
+ Author Affiliations
- Author Affiliations

A College of Animal Science and Technology, Shandong Agricultural University, Tai’an 271018, China.

B Corresponding author. Email: chlf@sdau.edu.cn

Animal Production Science 57(1) 90-95 https://doi.org/10.1071/AN14933
Submitted: 8 November 2014  Accepted: 31 August 2015   Published: 22 January 2016

Abstract

An experiment was conducted to determine the effects of varying the digestible energy (DE) density on growth performance, meat quality, caecum fermentation and microbiota in 3–5-month-old Rex rabbits. One-hundred and eighty 3-month-old Rex rabbits were allocated in individual cages to five treatments with diets having DE levels (MJ/kg as-fed basis) of 9.46, 9.97, 10.46, 10.94 and 11.43, respectively. The results showed as follows: with increasing DE concentration the average daily gain, muscle pH value of 45 min post-mortem, caecal pH value and NH3-N concentration increased (P < 0.05). Bacterial denaturing gradient gel electrophoresis (denaturing gradient gel electrophoresis) of partial 16S rRNA genes profiles were clearly different among different treatments, which suggests that the population of microorganism in the caecum was influenced by the dietary DE density.

Additional keywords: caecum fermentation, digestible energy, growth performance, meat quality, microbiota, Rex rabbits.


References

Abecia L, Fondevila M, Balcells J, Edwards JE, Newbold CJ, McEwan NR (2005) Molecular profiling of bacterial species in the rabbit caecum. FEMS Microbiology Letters 244, 111–115.
Molecular profiling of bacterial species in the rabbit caecum.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXhsVOnsLs%3D&md5=b3b1574e6917970616740926c991ffe9CAS | 15727829PubMed |

Bennegadi N, Fonty G, Millet L, Gidenne T, Licois D (2003) Effects of age and dietary fibre level on caecal microbial communities of conventional and specific pathogen-free rabbits. Microbial Ecology in Health and Disease 15, 23–32.
Effects of age and dietary fibre level on caecal microbial communities of conventional and specific pathogen-free rabbits.Crossref | GoogleScholarGoogle Scholar |

Bianchi M, Petracci M, Cavani C (2006) Effects of dietary inclusion of dehydrated lucerne and whole linseed on rabbit meat quality. World Rabbit Science 14, 247–258.

Bianchi M, Petracci M, Cavani C (2009) The influence of linseed on rabbit meat quality. World Rabbit Science 17, 97–107.

Blas E, Gidenne T (1998) Digestion of starch and sugars. In ‘The nutrition of the rabbit’. (Eds C de Blas, J Wiseman) pp. 17–38. (CABI Publishing: Wallingford, UK)

Blasco A, Ouhayoun J (1993) Harmonization of criteria and terminology in rabbit meat research. Revised proposal. World Rabbit Science 4, 93–99.

Boulahrouf A, Fonty G, Gouet P (1991) Establishment, counts, and identification of the fibrolytic microflora in the digestive tract of rabbit. Influence of feed cellulose content. Current Microbiology 22, 21–25.
Establishment, counts, and identification of the fibrolytic microflora in the digestive tract of rabbit. Influence of feed cellulose content.Crossref | GoogleScholarGoogle Scholar |

Chao H, Li FC (2008) Effect of level of fibre on performance and digestion traits in growing rabbits. Animal Feed Science and Technology 144, 279–291.
Effect of level of fibre on performance and digestion traits in growing rabbits.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXnvVKjs7o%3D&md5=c48fae00d5a76e2fd143379771bbfde5CAS |

Cheeke PR (1994) Nutrition and nutritional diseases. The biology of the laboratory rabbit 2, 321–323.

Committee on Animal Nutrition, National Research Council (1977) Nutrient requirements of rabbits, 2nd revised edn. The National Academies Press. Available at: http://www.nap.edu/catalog/35/nutrient-requirements-of-rabbits-second-revised-edition-1977 [verified 7 September 2015] .

Costello E, Stagaman KK, Dethlefsen L, Bohannan BJ, Relman DA (2012) The application of ecological theory toward an understanding of the human microbiome. Science 336, 1255–1262.
The application of ecological theory toward an understanding of the human microbiome.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38XnvFekt7k%3D&md5=8e9bccfb99fe2b77144c0c0a145199f5CAS | 22674335PubMed |

de Blas C, Mateos G (1998) Feed formulation. In ‘The nutrition of the rabbit’. (Eds C de Blas, J Wiseman) pp. 241–253. (CABI Publishing: Wallingford, UK)

Fonty G, Gouet P, Riou Y (1979) Effect of milk composition on the gastrointestinal microflora of artificially reared young rabbits. Annual Biology Animal Biochemistry and Biophysics 19, 567–571.
Effect of milk composition on the gastrointestinal microflora of artificially reared young rabbits.Crossref | GoogleScholarGoogle Scholar |

Gheisari A, Saraeian O, Toghiani M (2005) Response of male broiler breeder chicks to diets containing different levels of energy, protein and sulfur amino acids. JWSS-Isfahan University of Technology 9, 185–195.

Gidenne T (1996) Nutritional and ontogenic factors affecting rabbit caeco-colic digestive physiology. In ‘Proceedings of the 6th World rabbit congress’. (Ed. F Lebas) pp. 1, 13–28. (World Rabbit Science Association: Toulouse)

Gidenne T, Jehl N, Segura M, Michalet-Doreau B (2002) Microbial activity in the caecum of the rabbit around weaning: impact of a dietary fibre deficiency and of intake level. Animal Feed Science and Technology 99, 107–118.
Microbial activity in the caecum of the rabbit around weaning: impact of a dietary fibre deficiency and of intake level.Crossref | GoogleScholarGoogle Scholar |

Gong JH, Si WD, Forster RJ, Huang RJ, Yu H, Yin YL, Yang CB, Han YM (2007) 16S rRNA gene-based analysis of mucosa-associated bacterial community and phylogeny in the chicken gastrointestinal tracts: from crops to ceca. FEMS Microbiology Ecology 59, 147–157.
16S rRNA gene-based analysis of mucosa-associated bacterial community and phylogeny in the chicken gastrointestinal tracts: from crops to ceca.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXht1egtrc%3D&md5=c90503da6fa6ffc51560cc789f602bf3CAS |

Gouet Ph, Fonty G (1979) Changes in the digestive microflora of holoxenic* rabbits from birth until adulthood. Annales de Biologie Animale, Biochimie, Biophysique 19, 553–566.
Changes in the digestive microflora of holoxenic* rabbits from birth until adulthood.Crossref | GoogleScholarGoogle Scholar |

Konstantinov SR, Zhu WY, Williams BA, Tamminga S, de Vos WM, Akkermans ADL (2003) Effects of fermentable carbohydrates on piglet faecal bacterial communities as revealed by denaturing gradient gel electorphoresis analysis of 16 S ribosomal DNA. FEMS Microbiology Ecology 43, 225–235.
Effects of fermentable carbohydrates on piglet faecal bacterial communities as revealed by denaturing gradient gel electorphoresis analysis of 16 S ribosomal DNA.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXht1Kmtrk%3D&md5=dbba62f21e0c6727b18975b20bd5785dCAS | 19719683PubMed |

Li M, Gong JH, Cottrill M, Yu H, de Lange V, Burton J, Topp E (2003) Evaluation of QIAamp® DNA Stool Mini Kit for ecological studies of gut microbiota. Journal of Microbiological Methods 54, 13–20.
Evaluation of QIAamp® DNA Stool Mini Kit for ecological studies of gut microbiota.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXjsVWmt7w%3D&md5=7e3977d920fcd2e96dc9a4c806c47641CAS | 12732417PubMed |

Mendes AA, Moreira J, Oliveira E, Garcia EA, Almeida M, Garcia RG (2004) Effect of dietary energy on performance, carcass yield and abdominal fat of broiler chickens. Revista Brasileira de Zootecnia 33, 2300–2307.
Effect of dietary energy on performance, carcass yield and abdominal fat of broiler chickens.Crossref | GoogleScholarGoogle Scholar |

Michelland RJ, Combes S, Monteils V, Cauquil L, Gidenne T, Fortun-Lamothe L (2010) Molecular analysis of the bacterial community in digestive tract of rabbit. Anaerobe 16, 61–65.
Molecular analysis of the bacterial community in digestive tract of rabbit.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXkvFKms7o%3D&md5=ab579bb832c0bd82dc1cff913556942fCAS | 19460451PubMed |

Muyzer G, Smalla K (1998) Application of denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology. Antonie van Leeuwenhoek 73, 127–141.
Application of denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1cXjsVaqtbs%3D&md5=c0d6f20c655dd065d31ba89dc7444a12CAS | 9602286PubMed |

Muyzer G, Waal EC, Uitterlinden AG (1993) Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Applied and Environmental Microbiology 59, 695–700.

Nizza A, Stanco G, Di Meo C, Marongiu ML, Taranto S, Cutrignelli ML, Juliano L (2002) Effect of pre-weaning solid feed and milk intake on caecal content characteristics and performance of rabbits around weaning. Italian Journal of Animal Science 1, 95–102.
Effect of pre-weaning solid feed and milk intake on caecal content characteristics and performance of rabbits around weaning.Crossref | GoogleScholarGoogle Scholar |

Nyachoti C, Omogbenigun F, Rademacher M, Blank G (2006) Performance responses and indicators of gastrointestinal health in early-weaned pigs fed low-protein amino acid-supplemented diets. Journal of Animal Science 84, 125–134.

Oliver MA, Guerrero L, Diaz I, Gispert M, Pla M, Blasco A (1997) The effect of fat-enriched diets on the perirenal fat quality and sensory characteristics of meat from rabbit. Meat Science 47, 95–103.
The effect of fat-enriched diets on the perirenal fat quality and sensory characteristics of meat from rabbit.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2sXns1yitbs%3D&md5=78936613c3d6a63b792d3c6575cab0d9CAS | 22062620PubMed |

Panda A, Niranjan M, Reddy B, Sharma R (2006) Influence of dietary energy on growth, immunecompetence and carcass characteristics of coloured broiler chickens. Animal Nutrition and Feed Technology 6, 115–121.

Peiretti PG, Meineri G (2008) Effects on growth performance, carcass characteristics, and the fat and meat fatty acid profile of rabbits fed diets with chia (Salvia hispanica L.) seed supplements. Meat Science 80, 1116–1121.
Effects on growth performance, carcass characteristics, and the fat and meat fatty acid profile of rabbits fed diets with chia (Salvia hispanica L.) seed supplements.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXhtFOqsrbM&md5=686c1196fb0a7209f51fc75dfdee0e2aCAS | 22063845PubMed |

Prohászka L, Szemerédi G (1984) Regulation of the metabolism of volatile fatty acids in the caecum of rabbits. Zentralblatt fur Veterinarmedizin. Reihe B. 31, 358–366.
Regulation of the metabolism of volatile fatty acids in the caecum of rabbits.Crossref | GoogleScholarGoogle Scholar |

Qiao Y, Sun J, Ding Y, Le G, Shi Y (2013) Alterations of the gut microbiota in high-fat diet mice is strongly linked to oxidative stress. Applied Microbiology and Biotechnology 97, 1689–1697.
Alterations of the gut microbiota in high-fat diet mice is strongly linked to oxidative stress.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXhvFGhtbY%3D&md5=27c201650a8b5b00aae080831b2b8003CAS | 22948953PubMed |

Sanguinetti C, Simpson A (1994) Rapid silver staining and recovery of PCR products separated on polyacrylamide gels. BioTechniques 17, 914–921.

SAS (1985) ‘SAS/STAT users guide: basics.’ (SAS Institute Inc.: Cary, NC)

Simpson JM, McCracken VJ, Gaskins HR, Mackie RI (2000) Denaturing gradient gel electrophoresis analysis of 16S ribosomal DNA amplicons to monitor changes in fecal bacterial populations of weaning pigs after introduction of Lactobacillus reuteri strain MM53. Applied and Environmental Microbiology 66, 4705–4714.
Denaturing gradient gel electrophoresis analysis of 16S ribosomal DNA amplicons to monitor changes in fecal bacterial populations of weaning pigs after introduction of Lactobacillus reuteri strain MM53.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3cXnvFyrtr4%3D&md5=55f2c2120fa55f6ebf95375529896ca8CAS | 11055913PubMed |

Tannock GW, Munro K, Harmsen HJ, Welling GW, Smart J, Gopal PK (2000) Analysis of the fecal microflora of human subjects consuming a probiotic product containing Lactobacillus rhamnosus DR20. Applied and Environmental Microbiology 66, 2578–2588.
Analysis of the fecal microflora of human subjects consuming a probiotic product containing Lactobacillus rhamnosus DR20.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3cXjvFWnsLo%3D&md5=50a13d8e09902c36ffd1256caa297b04CAS | 10831441PubMed |

Weatherburn M (1967) Phenol-hypochlorite reaction for determination of ammonia. Analytical Chemistry 39, 971–974.
Phenol-hypochlorite reaction for determination of ammonia.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaF2sXksFSqtLY%3D&md5=1a9df262eaae8b60e7a9f9bfc7fa91e3CAS |

Xiong BH, Pang ZH, Luo QY (2009) Tables of feed composition and nutritive values in China. Chinese Feed 21, 28–37.

Zhou M, Hernandez-Sanabria E (2010) Characterization of variation in rumen methanogenic communities under different dietary and host feed efficiency conditions, as determined by PCR-denaturing gradient gel electrophoresis analysis. Applied and Environmental Microbiology 76, 3776–3786.
Characterization of variation in rumen methanogenic communities under different dietary and host feed efficiency conditions, as determined by PCR-denaturing gradient gel electrophoresis analysis.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXptVenurY%3D&md5=2ff4bec4c2c1bb47ec3bc0d403338ff8CAS | 20418436PubMed |

Zoetendal EG, Akkermans AD, De Vos WM (1998) Temperature gradient gel electrophoresis analysis of 16S rRNA from human fecal samples reveals stable and host-specific communities of active bacteria. Applied and Environmental Microbiology 64, 3854–3859.