Effects of supplementary bee pollen and its polysaccharides on nutrient digestibility and serum biochemical parameters in Holstein calves
Yan Tu A , Guo-Feng Zhang A , Kai-Dong Deng B , Nai-Feng Zhang A and Qi-Yu Diao A C
+ Author Affiliations
- Author Affiliations
A Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
B College of Animal Science, Jinling Institute of Technology, Nanjing, Jiangsu 210038, China.
C Corresponding author. Email: diaoqiyu@caas.cn
Animal Production Science 55(10) 1318-1323 https://doi.org/10.1071/AN14684
Submitted: 19 February 2014 Accepted: 8 August 2014 Published: 28 October 2014
Abstract
This study investigated the effects of supplementing the diets of 14–70-day calves with dietary bee pollen (BP) or its polysaccharides (PS) on growth performance, serum biochemical parameters, and apparent nutrient digestibility. Twenty-five newborn Holstein female calves were randomly offered one of the five diets (five calves per diet): a milk replacer supplemented with 0 (Control), 10 (10BP), 25 (25BP), or 50 g/day (50BP) bee pollen, or 5 g/day (5PS) bee pollen polysaccharides. The trial lasted 56 days, during which growth performance, apparent nutrient digestibility, and serum biochemical parameters were measured. Calves fed diets with BP had a greater bodyweight gain. Supplementation with BP or PS had significant effects on the apparent digestibility of DM, Ca and P in calves (P = 0.025, 0.012, and 0.076, respectively). The apparent DM digestibility of calves from group 25BP or 5PS was increased by 9.7% or 8.2% (P = 0.007 or 0.019), respectively, compared with that in the Control group. No significant differences were observed in serum concentrations of alkaline phosphatase, urea nitrogen, glucose, total protein, albumin, total cholesterol and triglycerides. These results demonstrated that dietary supplementation of BP at 25 g/day·calf or PS at 5 g/day·calf improved nutrient digestibility in pre-ruminant calves.
Additional keywords: bee pollen, dairy calf, pollen polysaccharide, serum biochemical parameters.
References
Almaraz-Abarca N, Campos MG, Ávila-Reyes JA, Naranjo-Jiménez N, Herrera-Corral J, González-Valdez LS (2004) Variability of antioxidant activity among honeybee-collected pollen of different botanical origin. Interciencia 29, 574–578.AOAC (1990) ‘Official methods of analysis.’ 15th edn. (Association of Official Analytical Chemists: Arlington, VA)
Brecker L, Wicklein D, Hermann M, Fuchs EC, Becker W-M, Petersen A (2005) Structural and immunological properties of arabinogalactan polysaccharides from pollen of timothy grass (Phleum pratense L.). Carbohydrate Research 340, 657–663.
| Structural and immunological properties of arabinogalactan polysaccharides from pollen of timothy grass (Phleum pratense L.).Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXhs1aiurs%3D&md5=593971c4c518b4e329de2c939ae4b427CAS | 15721337PubMed |
Campos MG, Webby RF, Markham KR, Mitchell KA, DaCunha AP (2003) Age-induced diminution of free radical scavenging capacity in bee pollens and contribution of constituent flavonoids. Journal of Agricultural and Food Chemistry 51, 742–745.
| Age-induced diminution of free radical scavenging capacity in bee pollens and contribution of constituent flavonoids.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXhs1On&md5=072ab2c6972549325b0e6e04c8ec537cCAS | 12537451PubMed |
Cannon SJ, Fahey GC, Murphy MR, Dikeman CL, Miller BL, Drackley JK (2010) Inclusion of psyllium in milk replacer for neonatal calves. 1. Effects on growth, digesta viscosity, rate of passage, nutrient digestibilities, and metabolites in blood. Journal of Dairy Science 93, 3652–3660.
| Inclusion of psyllium in milk replacer for neonatal calves. 1. Effects on growth, digesta viscosity, rate of passage, nutrient digestibilities, and metabolites in blood.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXht1GgtbfF&md5=26b09af08efc520ea341c62f5597399bCAS | 20655435PubMed |
Dai J, Wu Y, Chen SW, Zhu S, Yin HP, Wang M, Tang J (2010) Sugar compositional determination of polysaccharides from Dunaliella salina by modified RP-HPLC method of precolumn derivatization with 1-phenyl-3-methyl-5-pyrazolone. Carbohydrate Polymers 82, 629–635.
| Sugar compositional determination of polysaccharides from Dunaliella salina by modified RP-HPLC method of precolumn derivatization with 1-phenyl-3-methyl-5-pyrazolone.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXhtVeks7vM&md5=e445ef2bcc93b09fd082158f6a69361fCAS |
Haro A, López-Aliaga I, Lisbona F, Barrionuevo M, Alférez MJ, Campos MS (2000) Beneficial effect of pollen and/or propolis on the metabolism of iron, calcium, phosphorus, and magnesium in rats with nutritional ferropenic anemia. Journal of Agricultural and Food Chemistry 48, 5715–5722.
| Beneficial effect of pollen and/or propolis on the metabolism of iron, calcium, phosphorus, and magnesium in rats with nutritional ferropenic anemia.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3cXntVOmtbw%3D&md5=4701a932847e2e7689615b3ec4e5fd5bCAS | 11087544PubMed |
Huai WH, Wang ZC, Wang QF (2005) Effects of bee pollen on growth performance and meat quality of growing-finishing swine. Modern Agricultural Science and Technology 12, 64–65. [In Chinese]
Kroyer G, Hegedus N (2001) Evaluation of bioactive properties of pollen extracts as functional dietary food supplement. Innovative Food Science & Emerging Technologies 2, 171–174.
| Evaluation of bioactive properties of pollen extracts as functional dietary food supplement.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXmtF2qtbk%3D&md5=29298781d3b1b21298b971cf73ed46ceCAS |
Li CY, Wang DZ (2005) Effects of bee pollen on feeding lactating dairy cows. Journal of Traditional Chinese Veterinary Medicine 4, 57 [In Chinese]
Li SP, Zhao KJ, Ji ZN, Song ZH, Dong TT, Lo CK, Cheung JK, Zhu SQ, Tsim KW (2003) A polysaccharide isolated from Cordyceps sinensis, a traditional Chinese medicine, protects PC12 cells against hydrogen peroxide-induced injury. Life Sciences 73, 2503–2513.
| A polysaccharide isolated from Cordyceps sinensis, a traditional Chinese medicine, protects PC12 cells against hydrogen peroxide-induced injury.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXmvV2rtLY%3D&md5=0fe83a46f02d27aae65da7c6d22d1206CAS | 12954458PubMed |
Li F, Yuan QP, Rashid F (2009) Isolation, purification and immunobiological activity of a new water-soluble bee pollen polysaccharide form Crataegus pinnatifida Bge. Carbohydrate Polymers 78, 80–88.
| Isolation, purification and immunobiological activity of a new water-soluble bee pollen polysaccharide form Crataegus pinnatifida Bge.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXosVGisrc%3D&md5=cbf11bd35bbc0895d3496e5506b5b303CAS |
Liebelt RA, Calcagnetti D (1999) Effects of a bee pollen diet on the growth of the laboratory rat. American Bee Journal 139, 390–395.
Linskens HF, Jorde W (1997) Pollen as food and medicine. A review. Economic Botany 51, 78–86.
| Pollen as food and medicine. A review.Crossref | GoogleScholarGoogle Scholar |
Morais M, Moreira L, Feás X, Estevinho LM (2011) Honeybee-collected pollen from five Portuguese Natural Parks: Palynological origin, phenolic content, antioxidant properties and antimicrobial activity. Food and Chemical Toxicology 49, 1096–1101.
| Honeybee-collected pollen from five Portuguese Natural Parks: Palynological origin, phenolic content, antioxidant properties and antimicrobial activity.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXkt12js7c%3D&md5=a1a0c25abb462e268fdd35a7a3d3667aCAS | 21291944PubMed |
Orzáez Villanueva MT, Díaz Marquina A, Bravo Serrano R, Blazquez Abellán G (2002) The importance of bee-collected pollen in the diet: a study of its composition. International Journal of Food Sciences and Nutrition 53, 217–224.
| The importance of bee-collected pollen in the diet: a study of its composition.Crossref | GoogleScholarGoogle Scholar | 11951585PubMed |
Pan K, Sun H, Gao ZH (2006) Effects of bee pollen polysaccharide on growth performance and chemical compositions of muscle on broilers. Journal of Feed Industry 27, 39–41. [In Chinese]
Roulston TH, Cane JH (2000) Pollen nutritional content and digestibility for animals. Plant Systematics and Evolution 222, 187–209.
| Pollen nutritional content and digestibility for animals.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3cXls1Ohu7Y%3D&md5=3544787ec0370de1f8c57dba11ad4bc1CAS |
Song YF, Wang J, Li SH, Shang HF (2005) Effects of bee pollen on the development of digestive gland of broilers. Animal Husbandry & Veterinary Medicine 37, 14–17. [In Chinese]
Terré M, Calvo MA, Adelantado C, Kocherd A, Bach A (2007) Effects of mannan oligosaccharides on performance and microorganism fecal counts of calves following an enhanced-growth feeding program. Animal Feed Science and Technology 137, 115–125.
| Effects of mannan oligosaccharides on performance and microorganism fecal counts of calves following an enhanced-growth feeding program.Crossref | GoogleScholarGoogle Scholar |
Tu ZC, Li M, Liu GX, Dou YX, Zhang B (2007) Study on determination of rape pollen polysaccharide content by phenol-sulfuric acid method. Sciences and Technology of Food Industry 28, 219–221. [In Chinese]
Wang QF, Cheng QF (2005) Effect of pollen on duodenum configuration and structure of growing-finishing swine. Journal of HuaiHai Institute of Technology 14, 59–62. [Natural Sciences Edition]
Wang J, Wang QF, Li SH (2004) Effect of bee pollen on the organizational structure of porcine small intestine of growing-finishing pigs. Journal of Cereal & Feed Industry 8, 41–42. [In Chinese]
Wang LG, Lu SM, Liu SF, Pan K, Wu HX, Yan WY, Zeng ZHJ (2005) Effects of honeybee pollen polysaccharide on productive and immune performance in meat fowl. Acta Agricultural Universitatis Jiangxiensis 27, 450–453. [In Chinese]
Wang J, Gu YF, Li SH, Fang ZS, Feng BM (2006) Effect of the bee pollen on histological structure of digestive organs of layer. Journal of AnHui Sciences and Technology University 20, 1–6. [In Chinese]
Wei K, Sun ZH, Yan ZG, Tan YL, Wang H, Zhu XL (2011) Effects of Taishan Pinus massoniana pollen polysaccharide on immune response of rabbit haemorrhagic disease tissue inactivated vaccine and on production performance of Rex rabbits. Vaccine 29, 2530–2536.
| Effects of Taishan Pinus massoniana pollen polysaccharide on immune response of rabbit haemorrhagic disease tissue inactivated vaccine and on production performance of Rex rabbits.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXjs1SisLs%3D&md5=65fec4d1e7b6583978ad219f0ba1cfa0CAS | 21295100PubMed |
Zeng ZJ, Liu SF, Pan K, Wu HX, Yang KJ (2004) Effects of pollen and propolis on productive and immune performance in meat fowls. Scientia Agricultura Sinica 5, 751–755. [In Chinese]
Zhang J, Wang ZW (2011) Arabinoxylan from Canna edulis Ker by-product and its enzymatic activities. Carbohydrate Polymers 84, 656–661.
| Arabinoxylan from Canna edulis Ker by-product and its enzymatic activities.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXhtVeis7Y%3D&md5=a94e4397cb3c730d872d91c64b32a5d9CAS |
Zhang GF, Diao QY, Tu Y (2009) Research on extraction condition of bee pollen polysaccharide of water lily. Journal of Feed Industry 30, 4–7. [In Chinese]
