Treating Bos indicus bulls with rumen transfaunation after 24 hours of transportation does not replete muscle glycogen
C. L. O. Leo-Penu A B , L. A. Fitzpatrick B , H. N. Zerby B C and A. J. Parker B DA Kupang State Agricultural Polytechnic, Timor, NTT, Indonesia.
B James Cook University, Discipline of Veterinary Science, Townsville, Qld 4812, Australia.
C Department of Animal Sciences, The Ohio State University, Columbus, OH 43210, USA.
D Corresponding author. Email: anthony.parker@jcu.edu.au
Animal Production Science 56(10) 1738-1744 https://doi.org/10.1071/AN14632
Submitted: 10 June 2014 Accepted: 25 March 2015 Published: 25 June 2015
Abstract
Twelve Bos indicus bulls were allocated to one of two treatment groups: rumen transfaunation (n = 6) given 10 kg of rumen fluid after transport or Control (n = 6), given 10 kg of deionised water after transport. Glycogen concentrations of the M. semimembranosus, M. semitendinosis and M. longisimus dorsi were measured before transport on Day –7 and after transport on Day 0, Day 1, Day 4 and Day 9. Feed intake, liveweight gain, plasma metabolites and electrolytes were recorded on the sampling days. Rumen transfaunation increased the dry matter intake of treated bulls compared with the bulls treated with deionised water. Rumen transfaunation had no effect on glycogen concentrations of the M. semimembranosus, M. semitendinosis and M. longisimus dorsi compared with the bulls treated with deionised water. The M. semimembranosus and the M. semitendinosis decreased in glycogen concentration immediately after transport on Day 0 but repleted to pre-transportation concentrations within 1 day. However, the M. longisimus dorsi demonstrated no change in glycogen concentration between the pre-transportation sample on Day –7 and Day 0 or Day 1 after transportation. The M. longisimus dorsi of the bulls increased in glycogen concentration between Day 1 and Day 4 after transportation. The bulls in this experiment demonstrated that muscle glycogen, as measured at the M. longissimus dorsi, cannot recover to sufficient concentrations (40–45 µmol of glycogen concentration per gram muscle) to ensure normal meat quality until between 1 and 4 days of rest when feeding Rhodes grass hay ad libitum.
Additional keywords: bacteria, digesta, energy, meat quality, microbes.
References
Bergman EN (1990) Energy contributions of volatile fatty-acids from the gastrointestinal-tract in various species. Physiological Reviews 70, 567–590.Cole NA, Hutcheson DP (1985) Influence of realimentation diet on recovery of rumen activity and feed intake in beef steers. Journal of Animal Science 61, 692–701.
Cottyn BG, Boucque CV (1968) Rapid method for the gas-chromatographic determination of volatile fatty acids in rumen fluid. Journal of Agricultural and Food Chemistry 16, 105–107.
| Rapid method for the gas-chromatographic determination of volatile fatty acids in rumen fluid.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaF1cXjs1Glsw%3D%3D&md5=fd25f03b460ce3b6f2ddc14a295790faCAS |
Crouse JD, Smith SB, Prior RL (1984) Bovine muscle glycogen as affected by fasting and refeeding. Journal of Animal Science 59, 384–387.
Dehority BA (1984) Evaluation of subsampling and fixation procedures used for counting rumen protozoa. Applied and Environmental Microbiology 48, 182–185.
Ezaki T, Li N, Kawamura Y (2006) The anaerobic Gram-positive cocci. The Prokaryotes Part 1, Section 1.2, 795–808.
Fluharty FL, Loerch SC, Dehority BA (1996) Effects of feed and water deprivation on ruminal characteristics and microbial population of newly weaned and feedlot-adapted calves. Journal of Animal Science 74, 465–474.
Galyean ML, Lee RW, Hubbert ME (1981) Influence of fasting and transit on ruminal and blood metabolites in beef steers. Journal of Animal Science 53, 7–18.
Gardner GE, McIntyre BL, Tudor GD, Pethick DW (2001) The impact of nutrition on bovine muscle glycogen metabolism following exercise. Australian Journal of Agricultural Research 52, 461–470.
| The impact of nutrition on bovine muscle glycogen metabolism following exercise.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXjsFShsrY%3D&md5=f039245801ce126ae8df21296068b52eCAS |
Goering HK, Van Soest PJ (1970) Forage fiber analyses (apparatus, reagents, procedures, and some applications), 101–564–920 (last edited on 2002/02/27 17:23:12 US/Mountain). (U.S. Agricultural Research Service: Washington).
Immonen K, Ruusunen M, Hissa K, Puolanne E (2000) Bovine muscle glycogen concentration in relation to finishing diet, slaughter and ultimate pH. Meat Science 55, 25–31.
Knee BW, Cummins LJ, Walker PJ, Kearney GA, Warner RD (2007) Reducing dark-cutting in pasture-fed beef steers by high-energy supplementation. Australian Journal of Experimental Agriculture 47, 1277–1283.
| Reducing dark-cutting in pasture-fed beef steers by high-energy supplementation.Crossref | GoogleScholarGoogle Scholar |
McVeigh JM, Tarrant PV (1982) Glycogen content and repletion rates in beef muscle, effect of feeding and fasting. The Journal of Nutrition 112, 1306–1314.
McVeigh JM, Tarrant PV, Harrington MG (1982) Behavioral stress and skeletal muscle glycogen metabolism in young bulls. Journal of Animal Science 54, 790–795.
Mertens DR, Allen M, Carmany J, Clegg J, Davidowicz A, Drouches M, Frank K, Gambin D, Garkie M, Gildemeister B, Jeffress D, Jeon CS, Jones D, Kaplan D, Kim GN, Kobata S, Main D, Moua X, Paul B, Robertson J, Taysom D, Thiex N, Williams J, Wolf M (2002) Gravimetric determination of amylase-treated neutral detergent fiber in feeds with refluxing in beakers or crucibles: collaborative study. Journal of AOAC International 85, 1217–1240.
Minson DJ (1984) Digestibility and voluntary intake by sheep of five Digitaria species. Australian Journal of Experimental Agriculture and Animal Husbandry 24, 494–500.
| Digestibility and voluntary intake by sheep of five Digitaria species.Crossref | GoogleScholarGoogle Scholar |
Parker AJ, Hamlin GP, Coleman CJ, Fitzpatrick LA (2003) Quantitative analysis of acid base balance in Bos indicus steers subjected to transportation of long duration. Journal of Animal Science 81, 1434–1439.
Pethick DW, Cummins L, Gardner GE, Knee BW, McDowell M, McIntyre BL, Tudor G, Walker PJ, Warner RD (1999) The regulation by nutrition of glycogen in the muscle of ruminants. Recent Advances in Animal Nutrition in Australia 12, 145–151.
Rager KD, George LW, House JK, DePeters EJ (2004) Evaluation of rumen transfaunation after surgical correction of left-sided displacement of the abomasum in cows. Journal of the American Veterinary Medical Association 225, 915–920.
| Evaluation of rumen transfaunation after surgical correction of left-sided displacement of the abomasum in cows.Crossref | GoogleScholarGoogle Scholar | 15485053PubMed |
Tarrant PV (1990) Transportation of cattle by road. Applied Animal Behaviour Science 28, 153–170.
| Transportation of cattle by road.Crossref | GoogleScholarGoogle Scholar |
Teke B, Akdag F, Ekiz B, Ugurlu M (2014) Effects of different lairage times after long distance transportation on carcass and meat quality characteristics of Hungarian Simmental bulls. Meat Science 96, 224–229.
| Effects of different lairage times after long distance transportation on carcass and meat quality characteristics of Hungarian Simmental bulls.Crossref | GoogleScholarGoogle Scholar | 23916957PubMed |
Tudor GD, Coupar FJ, Pethick DW (1996) Effect of silage diets on glycogen concentration in the muscle of yearling cattle. Proceedings of the Australian Society of Animal Production 21, 451
Wulf DM, Emnett RS, Leheska JM, Moeller SJ (2002) Relationships among glycolytic potential, dark cutting (dark, firm, and dry) beef, and cooked beef palatability. Journal of Animal Science 80, 1895–1903.
