Effect of cold exposure on plasma glucose and acetate turnover rates in sheep
M. Al-Mamun A B C , Y. Sako A and H. Sano AA Department of Animal Science, Faculty of Agriculture, Iwate University, Morioka, Iwate, Japan.
B Department of Animal Nutrition, Bangladesh Agricultural University, Mymensingh, Bangladesh.
C Corresponding author. Email: mamunshimu@yahoo.com
Animal Production Science 54(10) 1728-1731 https://doi.org/10.1071/AN14406
Submitted: 15 March 2014 Accepted: 23 June 2014 Published: 19 August 2014
Abstract
Isotopic dilution methodology of [U-13C]glucose and [1-13C]Na-acetate were performed simultaneously as primed continuous infusions to determine plasma glucose and acetate metabolism in sheep during thermoneutral (TN) and cold exposure (CE, 2−4°C). The experiment was performed using crossbred (Corriedale × Suffolk) sheep (n = 5; 3 male and 2 female; ~2 years old, 38 ± 2.5 kg of initial bodyweight). The animals were offered mixed hay of orchardgrass and reed canarygrass (40 : 60) 62 g/kg0.75/day with ad libitum water access. Concentration of plasma glucose was determined enzymatically using the glucose oxidase method. Plasma concentration of non-esterified fatty acids (NEFA) and glucose were higher (P = 0.01 and P = 0.05 respectively) during CE than TN. Plasma concentration and the turnover rate of acetate were numerically higher (P = 0.09 and P = 0.25 respectively) during CE than during TN. Plasma glucose turnover rate was higher (P = 0.02) during CE than TN. The present findings suggested that plasma acetate turnover rate tended to be elevated during CE, with further more extensive studies required to clarify the significance.
Additional keywords: plasma nutrients metabolism, stable isotope.
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