Egg quality and production performance of laying hens injected with growth hormone and testosterone in the late phase of production
H. Mohammadi A G , Z. Ansari-Pirsaraei B , S. N. Mousavi C , M. Bouyeh D , A. Gholibeikifard E , P. Nouri F , A. Hatefi F and M. Rahmani A
+ Author Affiliations
- Author Affiliations
A Department of Agriculture, Payame Noor University, PO Box 19395–3697, Tehran, Iran.
B Department of Animal Science, Sari Agricultural Sciences and Natural Resources University, Sari, Iran.
C Department of Animal Science, Varamin-Pishva Branch, Islamic Azad University, Varamin, Iran.
D Department of Animal Science, Rasht Branch, Islamic Azad University, Rasht, Iran.
E Department of Animal Science, Technical and Vocational University, Tehran, Iran.
F MSc graduated in Animal Science.
G Corresponding author. Email: mohammadyhm@yahoo.com
Animal Production Science 56(1) 147-152 https://doi.org/10.1071/AN14111
Submitted: 24 November 2013 Accepted: 19 September 2014 Published: 20 January 2015
Abstract
In this study, 160 73-week-old laying hens (HyLine W-36) were used to investigate the changes in production performance and egg quality parameters of aged laying hens after injection of different doses of growth hormone (GH) and a fixed dose of testosterone (Ts). The hens were weighed individually, placed in laying cages and then randomly assigned to four treatments with four replicates of 10 birds each, in a completely randomised design. The experiment was started at the 75th week and lasted until the 82nd week of age. The treatments were: 1: injection of 100 µL distilled water (control group), 2: injection of 500 µg Ts/kg liveweight + 50 µg GH/kg liveweight, 3: injection of 500 µg Ts/kg liveweight + 100 µg GH/kg liveweight and 4: injection of 500 µg Ts/kg liveweight + 150 µg GH/kg liveweight. In this study, ovulation rate (egg production percent), egg mass and feed intake were significantly higher in Treatment 3 than the control group (P < 0.05), and in Treatment 4, feed conversion ratio was significantly higher than for the other experimental groups (P < 0.05). In addition, the birds in Treatment 3 showed greater egg weight, albumen height, eggshell weight, eggshell density and eggshell weight per surface area than the control group (P < 0.05). In Treatment 3, egg length significantly increased compared with Treatments 1 and 4 (P < 0.05), and in Treatments 2 and 4, shell breaking strength was significantly lower than for the control group (P < 0.05). In conclusion, our results showed positive effects of GH and Ts administration on egg quality parameters and production performance in aged laying hens. Taken together, in the present study Treatment 3 was found more effective in improving egg quality and production performance of the old laying hens.
Additional keywords: egg quality, growth hormone, old laying hens, production performance, testosterone.
References
Alaw-Qotbi A, Ansari-Pirsaraei Z, Seidavi A (2012) Investigation on exogenous testosterone and growth hormone injection on liver and ovary IGF-I gene expression and thyroid hormones of the native breeder hens. Annals of Biological Research 3, 1102–1108.Al-Massad M, Al-Shdefat R, Khashroum A (2011) The effect of microbial phytase and dietary calcium level on the performance and eggshell quality in laying hens fed marginal phosphorus diets. Asian Journal of Animal Science 5, 118–126.
| The effect of microbial phytase and dietary calcium level on the performance and eggshell quality in laying hens fed marginal phosphorus diets.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXnslCjs7o%3D&md5=a94f069ad93a8202d012366349611d69CAS |
Alkan S, Karabag K, Galic A, Balcioglu MS (2008) Predicting yolk height, yolk width, albumen length, eggshell weight, egg shape index, eggshell thickness, egg surface area of Japanese quails using various eggs traits as regressors. International Journal of Poultry Science 7, 85–88.
| Predicting yolk height, yolk width, albumen length, eggshell weight, egg shape index, eggshell thickness, egg surface area of Japanese quails using various eggs traits as regressors.Crossref | GoogleScholarGoogle Scholar |
Ansari-Pirsaraei Z (2009) Effect of testosterone and growth hormone injection on expression of IGF-I, type-I IGFR and type-II IGFBP genes of the Mazandaran native breeder hens. PhD Thesis, University of Tehran.
Ansari-Pirsaraei Z, Shahneh AZ, Zaghari M, Zamiri MJ, Mianji GR (2008) Effect of testosterone and growth hormone injection before puberty on follicles size, rate of egg production and egg characteristics of the Mazandaran native breeder hens. African Journal of Biotechnology 7, 3149–3154.
Ansari-Pirsaraei Z, Mianji GR, Shahneh AZ, Zaghari M, Zamiri MJ (2010a) Effect of testosterone and growth hormone injection before puberty on egg quality, egg production and some blood parameters of native breeder hens. In ‘Proceedings of the 2010 international Conference on Agricultural and Animal Science (CAAS)’. Singapore, 26–28 February. pp. 113–117. World Academic Union (World Academic Press)
Ansari-Pirsaraei Z, Shahneh AZ, Zaghari M, Zamiri MJ, Mianji GR (2010b) Effect of stimulation of follicle growth and egg production of breeder hens with hormone injection. In ‘International conference on chemistry and chemical engineering (ICCCE)’. Kyoto, Japan, 1–3 August. pp. 339–341. (Institute of Electrical and Electronics Engineers).
Bachelot A, Monget P, Imbert-Bollore P, Coshigano K, Kopchick JJ, Kelly P, Binart N (2002) Growth hormone is required for ovarian follicular growth. Endocrinology 143, 4104–4112.
| Growth hormone is required for ovarian follicular growth.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38Xnt1ygt7Y%3D&md5=64ed5c333c12c9eb77ea00b0e4717addCAS | 12239122PubMed |
Christians JK, Williams TD (1999) Effects of exogenous 17b-estradiol on the reproductive physiology and reproductive performance of European starlings (Sturnus vulgaris). The Journal of Experimental Biology 202, 2679–2685.
Croze F, Etches RJ (1980) The physiological significance of androgen-induced ovulation in the hen. The Journal of Endocrinology 84, 163–171.
| The physiological significance of androgen-induced ovulation in the hen.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL3cXhsVSqsrk%3D&md5=d6918f106ad4f08b4c4a79b05fdb963cCAS | 7359077PubMed |
Ebeid TA, Eid YZ, El-Abd EA, El-Habbak MM (2008) Effects of catecholamines on ovary morphology, blood concentrations of estradiol-17β, progesterone, zinc, triglycerides and rate of ovulation in domestic hens. Theriogenology 69, 870–876.
| Effects of catecholamines on ovary morphology, blood concentrations of estradiol-17β, progesterone, zinc, triglycerides and rate of ovulation in domestic hens.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXktFCltr8%3D&md5=b3d7ae1e4bc8d8541248db9fffed843fCAS | 18316117PubMed |
EL-Husseiny OM, Abd-Elsamee MO, Hassane MI, Omara II (2008) Response of egg production and egg shell quality to dietary vegetable oils. International Journal of Poultry Science 7, 1022–1032.
Fayeye TR, Adeshiyan AB, Olugbami AA (2005) Egg traits, hatchability and early growth performance of the fulani-ecotype chicken. Livestock Research for Rural Development 17, Art. #94
Fraps RM (1955) Egg production and fertility in poultry. In ‘Progress in the physiology of farm animals’. Vol. 2. (Ed. J Hammond) pp. 671–740. (Butterworths: London)
Goerlich VC, Dijkstra C, Groothuis TGG (2010) Effects of in vivo testosterone manipulation on ovarian morphology, follicular development, and follicle yolk testosterone in the homing pigeon. The Journal of Experimental Zoology 313A, 328–338.
| Effects of in vivo testosterone manipulation on ovarian morphology, follicular development, and follicle yolk testosterone in the homing pigeon.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXpsFSltb4%3D&md5=d727577401f09cf4b21ef7bbff94634fCAS |
Johnson AL (2000) Reproduction in the female. In ‘Sturkie’s avian physiology’. (Ed. GC Whittow) pp. 569–596. (Academic Press: San Diego, London, Boston)
Kelley AJ (2003) The effects of storage time on vitelline membrane protein banding patterns and interior egg quality of eggs from non-molted and molted hens. M.Sc. Thesis, Food Science and Technology Faculty, Texas A&M University.
Lebedeva IY, Lebedev VA, Grossmann R, Parvizi N (2010) Age-dependent role of steroids in the regulation of growth of the hen follicular wall. Reproductive Biology and Endocrinology 8, 15
| Age-dependent role of steroids in the regulation of growth of the hen follicular wall.Crossref | GoogleScholarGoogle Scholar | 20156346PubMed |
McNabb FN (2000) Thyroids. In ‘Sturkie’s avian physiology’. (Ed. GC Whittow) pp. 461–471. (Academic Press: London)
Mohammadi H, Ansari-Pirsaraei Z (2014) Changes in some blood parameters and production performance of old laying hens due to growth hormone and testosterone injection. Journal of Animal Physiology and Animal Nutrition 98, 483–490.
| Changes in some blood parameters and production performance of old laying hens due to growth hormone and testosterone injection.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2cXnslCktL0%3D&md5=2dd6e263a87e2e262408e665cacd6492CAS | 23808354PubMed |
Narushin V (2005) Egg geometry calculation using the measurements of length and breadth. Poultry Science 84, 482–484.
| Egg geometry calculation using the measurements of length and breadth.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD2M7ltVygsQ%3D%3D&md5=9af61696877d620a96d6006b05f86c41CAS | 15782919PubMed |
National Research Council (1994) ‘Nutrient requirement of poultry.’ 9th edn. (The National Academies Press: Washington, DC)
Niv-Spector L, Raver N, Friedman-Einat M, Grosclaude J, Gussakovsky E, Livnah O, Gertler A (2005) Mapping leptin-interacting sites in recombinant leptin-binding domain (LBD) subcloned from chicken leptin receptor. Biochemical Journal 390, 475–484.
| Mapping leptin-interacting sites in recombinant leptin-binding domain (LBD) subcloned from chicken leptin receptor.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXoslKgtLo%3D&md5=52ecbf06232c02ffd9e8d9b2574c771cCAS | 15842201PubMed |
Onagbesan OM, Metayer S, Tona K, Williams J, Decuypere E, Bruggeman V (2006) Effects of genotype and feed allowance on plasma luteinizing hormones, follicle-stimulating hormones, progesterone, estradiol levels, follicle differentiation, and egg production rates of broiler breeder hens. Poultry Science 85, 1245–1258.
| Effects of genotype and feed allowance on plasma luteinizing hormones, follicle-stimulating hormones, progesterone, estradiol levels, follicle differentiation, and egg production rates of broiler breeder hens.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XntVyktrs%3D&md5=4677a788109f4c1d57ab881502e6979dCAS | 16830866PubMed |
Ottinger MA, Abdelnabi M, Li Q, Chen K, Thompson N, Harada N, Panzica CV, Panzica GC (2004) The Japanese quail: a model for studying reproductive aging of hypothalamic systems. Experimental Gerontology 39, 1679–1693.
| The Japanese quail: a model for studying reproductive aging of hypothalamic systems.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2cXhtVCltLbF&md5=ba9f78605e12f401c8681c66e48f98d9CAS | 15582284PubMed |
Pelicia K, Garcia EA, Faitarone ABG, Silva AP, Berto DA, Molino AB, Vercese F (2009) Calcium and available phosphorus levels for laying hens in second production cycle. Brazilian Journal of Poultry Science 11, 39–49.
Rayan GN, Galal A, Fathi MM, El-Attar AH (2010) Impact of layer breeder flock age and strain on mechanical and ultrastructural properties of eggshell in chicken. International Journal of Poultry Science 9, 139–147.
| Impact of layer breeder flock age and strain on mechanical and ultrastructural properties of eggshell in chicken.Crossref | GoogleScholarGoogle Scholar |
Richards MP, Proszkowiec-Weglarz M (2007) Mechanisms regulating feed intake, energy expenditure, and body weight in poultry. Poultry Science 86, 1478–1490.
| Mechanisms regulating feed intake, energy expenditure, and body weight in poultry.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXot1Crsrs%3D&md5=94e57ddf8421c6762a9be90371df29aaCAS | 17575199PubMed |
Shen X, Steyrer E, Retzek H, Sanders EJ, Schneider WJ (1993) Chicken oocyte growth: receptor-mediated yolk deposition. Cell and Tissue Research 272, 459–471.
| Chicken oocyte growth: receptor-mediated yolk deposition.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK3sXltFektLo%3D&md5=3b7046cff445b3a6afc5d41692dc1657CAS | 8393385PubMed |
Tienhoven AV (1961) Endocrinology of reproduction in birds. In ‘Sex and internal secretions’. (Ed. WC Baltimore) pp. 1088–1169. (Williams & Wilkins: Baltimore, MD)
Tomas FM, Pym RA, McMurtry JP, Francis GL (1998) Insulin-like growth factor (IGF)-I but not IGF-II promotes lean growth and feed efficiency in broiler chickens. General and Comparative Endocrinology 110, 262–275.
| Insulin-like growth factor (IGF)-I but not IGF-II promotes lean growth and feed efficiency in broiler chickens.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1cXjsFGqtb0%3D&md5=8bab15be067a6eeccb3cef1a28de11e6CAS | 9593647PubMed |
Tonetta SA, diZerega GS (1989) Intragonadal regulation of follicular maturation. Endocrine Reviews 10, 205–229.
| Intragonadal regulation of follicular maturation.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL1MXkslSqu7Y%3D&md5=dd9c17021d1b4c956513e986f7edd4f2CAS | 2473895PubMed |
Wu G, Gunawardana P, Bryant M, Voitle R, Roland D (2008) Effect of molting method and dietary energy on postmolt performance of two strains of single comb white leghorn hens. Journal of Applied Poultry Research 17, 1–10.
| Effect of molting method and dietary energy on postmolt performance of two strains of single comb white leghorn hens.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXos1yqur4%3D&md5=99548686de2516b95662f34b68020bdfCAS |
Zakaria AH, Plumstead PW, Sanchez HR, Leksrisompong N, Osborne J, Brake J (2005) Oviposition pattern, egg weight, fertility, and hatchability of young and old broiler breeders. Poultry Science 84, 1505–1509.
| Oviposition pattern, egg weight, fertility, and hatchability of young and old broiler breeders.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD2MrjtFKgtQ%3D%3D&md5=18a6e17debe812ae40338d68ddd38e81CAS | 16206576PubMed |
Zou XT, Xu ZR, Zhu JL, Fang XJ, Jiang JF (2007) Effects of dietary dihydropyridine supplementation on laying performance and fat metabolism of laying hens. Asian-Australasian Journal of Animal Sciences 20, 1606–1611.
| Effects of dietary dihydropyridine supplementation on laying performance and fat metabolism of laying hens.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXhtFSksr3I&md5=663a0ac6e7cb41a7d23b1f641e2f88dcCAS |
