Evaluation of fructosamine as a new biomarker for diagnosis of hepatic lipidosis in dairy cows
Masoud Mostafavi A , Hesam A. Seifi A B D , Mehrdad Mohri A B and Abdullah Jamshidi CA Department of Clinical Sciences, School of Veterinary Medicine, Ferdowsi University of Mashhad, PO Box 91775-1793, Mashhad, Iran.
B Centre of Excellence in Ruminant Abortion and Neonatal Mortality, Ferdowsi University of Mashhad, PO Box 91775-1793, Mashhad, Iran.
C Department of Food Hygiene, School of Veterinary Medicine, Ferdowsi University of Mashhad, PO Box 91775-1793, Mashhad, Iran.
D Corresponding author. Email: haseifi@um.ac.ir
Animal Production Science 55(8) 1005-1010 https://doi.org/10.1071/AN14108
Submitted: 21 February 2014 Accepted: 27 May 2014 Published: 18 September 2014
Abstract
An abattoir-based cohort study using liver and serum specimens from Holstein dairy cows (452 healthy and 54 fatty liver cases) was conducted. Serum fructosamine and other biochemical parameters and fat content of liver specimens were measured. There were significant negative correlations of fructosamine with hepatic lipid content (P = 0.001), and serum total bilirubin (P = 003) and significant positive correlation with glucose (P < 0.02), albumin (P < 0.0001) and cholesterol (P < 0.0001) within normal cows. In fatty liver-affected cows significant positive correlations were seen between fructosamine and cholesterol (P < 0.0001) and albumin (P < 0.0001). In addition fructosamine had a significant negative correlation with the ratio of non-esterified fatty acids to cholesterol in both normal (P < 0.0001) and fatty liver-affected cows (P = 0.006). We found that serum fructosamine concentration was lower (P < 0.001) in cows suffering from fatty liver. The area under the receiver operating characteristic curve of fructosamine for diagnosis of fatty liver was 67.6. Optimum fructosamine cut-point based on the maximum total of sensitivity (SE) and specificity (SP) was <213 µmol/L (SE = 71%; SP = 65). Cows with serum fructosamine concentrations below 213 µmol/L were 4.5 times more likely to have hepatic lipidosis (odds ratio = 4.5; 95% CI = 2.4–8.6; P < 0.0001). We found that fructosamine showed a combination of high SE, SP, which was greater than aspartate aminotransferase, non-esterified fatty acids, β-hydroxybutyrate, total cholesterol, bile acids and bilirubin. In addition, fructosamine showed a positive likelihood ratio of 2.0, and negative likelihood ratio of 0.45. In conclusion, the present results indicate that fructosamine measurement could improve the diagnosis of fatty liver in dairy cows.
Additional keywords: fatty liver, liver function.
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