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RESEARCH ARTICLE
Contents Vol 48(5)

Effect of surface applied glycine betaine on herbage production and quality of perennial ryegrass–white clover pastures

J. M. Lee A F , K. Elborough B E , W. D. Catto C , D. J. Donaghy D and J. R. Roche A
+ Author Affiliations
- Author Affiliations

A DairyNZ, Private Bag 3221, Hamilton 3240, New Zealand.

B ViaLactia BioSciences, PO Box 109185, Auckland 1061, New Zealand.

C Ballance Agri-Nutrients Limited, Private Bag 12503, Mount Maunganui 3116, New Zealand.

D University of Tasmania, PO Box 3253, Burnie, Tas. 7320, Australia.

E Present address: HortResearch, 120 Mt Albert Road, Auckland 1025, New Zealand.

F Corresponding author. Email: julia.lee@dairynz.co.nz

Australian Journal of Experimental Agriculture 48(5) 687-694 https://doi.org/10.1071/EA07123
Submitted: 3 May 2007  Accepted: 25 November 2007   Published: 7 April 2008

Abstract

Osmoprotectants have been reported to reduce the detrimental effects of various environmental stresses in many different plant species. However, there is little research available concerning pasture species. Two experiments were undertaken with the aim of quantifying the effect of surface applications of exogenous glycine betaine (GB) on herbage production and quality of perennial ryegrass (Lolium perenne L.)–white clover (Trifolium repens L.) pastures during periods of moisture stress and cold temperatures over 2 years. Pastures fertilised with GB were compared with unfertilised pastures and pastures fertilised with nitrogen (N). Rates of 0.5, 1.0 and 1.5 kg GB/ha.defoliation were applied in experiment 1 and 5 kg GB/ha.defoliation was applied in experiment 2. Surface applications of GB did not significantly affect herbage production relative to unfertilised pastures; herbage yields averaged 12 248 and 12 693 kg DM/ha over 11 months in experiment 1, and 7253 and 7177 kg DM/ha over 6 months during summer and autumn in experiment 2, for the unfertilised control and GB, respectively. During both experiments, herbage quality parameters were not affected by GB application, although the proportion of white clover in the sward between summer and winter during experiment 1 was greater (P < 0.01) in plots treated with GB than in untreated plots. Application of N fertiliser increased (P < 0.001) herbage production, but did not consistently affect herbage quality. The failure of surface applications of exogenous GB to improve the herbage production or quality of perennial ryegrass–white clover pastures suggests that it is not an appropriate method to enhance plant tolerance to environmental stress at the concentrations applied in these studies.

Additional keywords: water deficit.


Acknowledgements

The authors would like to acknowledge the statistical expertise of Barbara Dow, the help afforded by Scott Farm staff and the technical expertise of Penny Aspin, Glenise Ferguson, Angela Sheahan and Talia and Jordan van der Wel. This project was funded by Ballance AgriNutrients.


References


Agboma PC, Jones MGK, Peltonen-Sainio P, Rita H, Pehu E (1997a) Exogenous glycinebetaine enhances grain yield of maize, sorghum and wheat grown under two supplementary watering regimes. Journal Agronomy & Crop Science 178, 29–37.
|  CAS |
open url image1

Agboma PC, Peltonen-Sainio P, Hinkkanen R, Pehu E (1997b) Effect of foliar application of glycinebetaine on yield components of drought-stressed tobacco plants. Experimental Agriculture 33, 345–352.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Agboma PC, Sinclair TR, Jokinen K, Peltonen-Sainio P, Pehu E (1997c) An evaluation of the effect of exogenous glycinebetaine on the growth and yield of soybean: timing of application, watering regimes and cultivars. Field Crops Research 54, 51–64.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Allard F, Houde M, Krol M, Ivanov A, Huner NPA, Sarhan F (1998) Betaine improves freezing tolerance in wheat. Plant & Cell Physiology 39, 1194–1202.
|  CAS |
open url image1

Borowitzka LJ (1981) Solute accumulation and regulation of cell water activity. In ‘The physiology and biochemistry of drought resistance in plants’. (Eds LG Paleg, D Aspinall) pp. 97–130. (Academic Press: Sydney)

Brereton AJ, Carton OT, O’Keefe WF (1985) Tissue turnover in perennial ryegrass (Lolium perenne L.) during winter. Irish Journal of Agricultural Research 24, 49–62. open url image1

Corson DC, Waghorn GC, Ulyatt MJ, Lee J (1999) NIRS: forage analysis and livestock feeding. Proceedings of the New Zealand Grassland Association 61, 127–132. open url image1

Datta SK (2002) Recent developments in transgenics for abiotic stress tolerance in rice. International Rice Research Institute, JIRCAS Working Report, Philippines.

Diaz-Zorita M, Fernandez-Canigia MV, Grosso GA (2001) Applications of foliar fertilizers containing glycinebetaine improve wheat yields. Journal Agronomy & Crop Science 186, 209–215.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Fulkerson WJ, Donaghy DJ (2001) Plant-soluble carbohydrate reserves and senescence – key criteria for developing an effective grazing management system for ryegrass-based pastures: a review. Australian Journal of Experimental Agriculture 41, 261–275.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Gay AP (1993) Leaf area and light interception. In ‘Sward measurement handbook’. (Eds A Davies, RD Baker, SA Grant, AS Laidlaw) pp. 121–138. (British Grassland Society: Reading, UK)

Genstat 7 (2004) ‘Genstat for Windows. Release 7.2.’ (VSN International Ltd: Oxford, UK)

Gorham J (1995) Betaines in higher plants – biosynthesis and role in stress metabolism. In ‘Amino acids and their derivatives in higher plants’. (Ed. RM Wallsgrove) pp. 171–203. (Cambridge University Press: Cambridge, UK)

Harinasut P, Tsutsui K, Takabe T, Nomura M, Takabe T, Kishitani S (1996) Exogenous glycinebetaine accumulation and increased salt-tolerance in rice seedlings. Bioscience, Biotechnology, and Biochemistry 60, 366–368.
|  CAS |
open url image1

Karsten HD, MacAdam JW (2001) Effect of drought on growth, carbohydrates, and soil water use by perennial ryegrass, tall fescue, and white clover. Crop Science 41, 156–166.
|  CAS |
open url image1

Kemp CD (1960) Methods of estimating the leaf area of grasses from linear measurements. Crop Science 24, 491–499. open url image1

Kishitani S, Watanabe K, Yasuda S, Arakawa K, Takabe T (1994) Accumulation of glycinebetaine during cold acclimation and freezing tolerance in leaves of winter and spring barley plants. Plant, Cell & Environment 17, 89–95.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Langer RHM (Ed.) (1979) ‘How grasses grow.’ 2nd edn. (Edward Arnold Ltd: London)

Larkum AWD, Wyn Jones RG (1979) Carbon dioxide fixation by chloroplasts isolated in glycinebetaine, a putative cytoplasmic osmoticum. Planta 145, 393–394.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Le Rudulier D, Strom AR, Dandekar AM, Smith LT, Valentine RC (1984) Molecular biology of osmoregulation. Science 224, 1064–1068.
| Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1

Lopez CML, Takahashi H, Yamazaki S (2002) Plant-water relations of kidney bean plants treated with NaCl and foliarly applied glycinebetaine. Journal Agronomy & Crop Science 188, 73–80.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Mäkelä P, Kleemola J, Jokinen K, Mantila J, Pehu E, Peltonen-Sainio P (1997) Growth response of pea and summer turnip rape to foliar application of glycinebetaine. Acta Agriculturæ Scandinavica Section B. Soil and Plant Science 47, 168–175. open url image1

McKenzie BA, Kemp PD, Moot DJ, Matthew C, Lucas RJ (2000) Environmental effects on plant growth and development. In ‘New Zealand pasture crop and science’. (Eds J White, J Hodgson) pp. 29–44. (Oxford University Press: Auckland, New Zealand)

Mickelbart MV, Ejeta G, Rhodes D, Joly RJ, Goldsbrough PB (1999) Assessing the contribution of glycinebetaine to environmental stress tolerance in sorghum. In ‘Molecular approaches for the genetic improvement of cereals for stable production in water-limited environments. An International Workshop, International Maize and Wheat Improvement Centre, El Batan, Mexico’. (Eds JM Ribaut, D Poland) pp. 86–89. (CIMMYT: Mexico)

Naidu BP, Stevenson G, Page R, Munford S (2003) Glycinebetaine foliar application increases pasture winter growth and milk yield. In ‘Solutions for a better environment. Proceedings of the 11th Australian agronomy conference, Geelong’. (Australian Society of Agronomy: Gosford, NSW)

Pospíšilová J (2003) Participation of phytohormones in the stomatal regulation of gas exchange during water stress. Biologia Plantarum 46, 491–506.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Rhodes D, Hanson AD (1993) Quaternary ammonium and tertiary sulfonium compounds in higher plants. Annual Review of Plant Physiology and Plant Molecular Biology 44, 357–384.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Rhodes D, Rich PJ, Brunk DG, Ju GC, Rhodes JC, Pauly MH, Hansen LA (1989) Development of two isogenic sweet corn hybrids differing for glycinebetaine content. Plant Physiology 91, 1112–1121.
|  CAS | PubMed |
open url image1

Roberts AHC, Morton JD (1999) ‘Fertiliser use on New Zealand dairy farms.’ (New Zealand Fertiliser Manufacturers’ Association: Auckland, New Zealand)

Roche JR, Morton JD (1995) The effect of closing date in autumn and turnout date in spring on sward characteristics, dry matter output and the milk performace of spring-calving dairy cows in early lactation. Masters Thesis. National University of Ireland, Dublin, Ireland.

Rulcová J (2000) Effect of cytokinins on plant recovery after water stress. PhD thesis, Charles University, Czech Republic.

Rulcová J, Pospíšilová J (2001) Effect of benzylaminopurine on rehydration of bean plants after water stress. Biologia Plantarum 44, 75–81.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Ryle GJA (1964) A comparison of leaf and tiller growth in seven perennial grasses as influenced by nitrogen and temperature. Journal of the British Grassland Society 19, 281–290.
|  CAS |
open url image1

Sakamoto A, Murata N (2000) Genetic engineering of glycinebetaine synthesis in plants: current status and implications for enhancement of stress tolerance. Journal of Experimental Botany 51, 81–88.
| Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1

Singleton PL (1991) ‘Soils of Ruakura – a window on the Waikato.’ (DSIR Land Resources: Lower Hutt, New Zealand)

Smith LT, Pocard J, Bernard T, Le Rudulier D (1988) Osmotic control of glycine betaine biosynthesis and degradation in Rhizobium meliloti. Journal of Bacteriology 170, 3142–3149.
|  CAS | PubMed |
open url image1

Soil Survey Staff (1990) ‘Keys to soil taxonomy.’ 4th edn. (United States Department of Agriculture: Blacksburg, VA)

Talwar HS, Yanagihara S (1999) Physiological basis of heat tolerance during flowering and pod setting stages in Groundnut (Arachis hypogaea L.). Japan International Research Center for Agricultural Sciences, Working report no. 14, Ibaraki, Japan.

Thomas JC, Sepahi M, Arendall B, Bohnert HJ (1995) Enhancement of seed germination in high salinity by engineering mannitol expression in Arabidopsis thaliana. Plant, Cell & Environment 18, 801–806.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Whitehead DC (1995) ‘Grassland nitrogen.’ (CAB International: Oxon, UK)

Wilman D, Fisher A (1996) Effects of interval between harvests and application of fertilizer N in spring on the growth of perennial ryegrass in a grass/white clover sward. Grass and Forage Science 51, 52–57.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Wilson JR (1981) Environmental and nutritional factors affecting herbage quality. In ‘Nutritional limits to animal production from pastures. Proceedings international symposium, St Lucia, Queensland’. (Ed. JB Hacker) pp. 111–131. (Commonwealth Agricultural Bureaux: Farnham Royal, UK)

Zhao Y, Aspinall D, Paleg LG (1992) Protection of membrane integrity in Medicago sativa L. by glycinebetaine against the effects of freezing. Journal of Plant Physiology 140, 541–543.
|  CAS |
open url image1