Detecting quantitative trait loci affecting beef tenderness on bovine chromosome 7 near calpastatin and lysyl oxidase
R. D. Drinkwater A D , Y. Li A D , I. Lenane A , G. P. Davis A D , R. Shorthose B , B. E. Harrison A D , K. Richardson A , D. Ferguson B D , R. Stevenson A D , J. Renaud A D , I. Loxton C , R. J. Hawken A , M. B. Thomas A , S. Newman A D , D. J. S. Hetzel A D and W. Barendse A EA CSIRO Livestock Industries, Level 5 Queensland Bioscience Precinct, 306 Carmody Road, St Lucia, Qld 4067, Australia.
B Food Science Australia, Cannon Hill, Qld 4170, Australia.
C Queensland Department of Primary Industries and Fisheries, Rockhampton, Qld 4701, Australia.
D CRC for the Cattle Industry (Beef Quality), Armidale, NSW 2350, Australia.
E Corresponding author. Email: bill.barendse@csiro.au
Australian Journal of Experimental Agriculture 46(2) 159-164 https://doi.org/10.1071/EA05185
Submitted: 28 June 2005 Accepted: 15 December 2005 Published: 3 March 2006
Abstract
From a study of 3 large half-sib families of cattle, we describe linkage between DNA polymorphisms on bovine chromosome 7 and meat tenderness. Quantitative trait loci (QTL) for Longissimus lumborum peak force (LLPF) and Semitendonosis adhesion (STADH) were located to this map of DNA markers, which includes the calpastatin (CAST) and lysyl oxidase (LOX) genes. The LLPF QTL has a maximum lodscore of 4.9 and allele substitution of approximately 0.80 of a phenotypic standard deviation, and the peak is located over the CAST gene. The STADH QTL has a maximum lodscore of 3.5 and an allele substitution of approximately 0.37 of a phenotypic standard deviation, and the peak is located over the LOX gene. This suggests 2 separate likelihood peaks on the chromosome. Further analyses of meat tenderness measures in the Longissimus lumborum, LLPF and LL compression (LLC), in which outlier individuals or kill groups are removed, demonstrate large shifts in the location of LLPF QTL, as well as confirming that there are indeed 2 QTL on bovine chromosome 7. We found that both QTL are reflected in both LLPF and LLC measurements, suggesting that both these components of tenderness, myofibrillar and connective tissue, are detected by both measurements in this muscle.
Acknowledgments
We thank Mike Goddard for statistical advice, and Belinda Norris and James Kijas for comments which improved the manuscript. We thank the Cooperative Research Centre for the Cattle Industry (Beef Quality) for financial support. These data were unpublished background Intellectual Property for the CAST3 SNP, which has been licenced for commercial testing as the GeneSTAR tenderness test.
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