Expression of imprinted genes surrounding the callipyge mutation in ovine skeletal muscle
T. Vuocolo A , N. E. Cockett B and R. L. Tellam A CA CSIRO Livestock Industries, Queensland Biosciences Precinct, 306 Carmody Road, St Lucia, Qld 4067, Australia.
B Utah State University, Logan, Utah, USA.
C Corresponding author. Email: Ross.Tellam@csiro.au
Australian Journal of Experimental Agriculture 45(8) 879-892 https://doi.org/10.1071/EA05049
Submitted: 14 February 2005 Accepted: 3 June 2005 Published: 26 August 2005
Abstract
The callipyge mutation in sheep results in postnatal hypertrophy and leanness of skeletal muscles in the pelvic limbs and loins. Associated changes also occur in the expression of a number of imprinted genes flanking the site of the mutation, which lies at the telomeric end of ovine chromosome 18. The transcripts from several of these genes are either spliced or undergo substantial RNA processing, sometimes in a very complex manner. The current investigation examined the effects of the callipyge mutation on the relative expression of some of these splice variants in samples taken: at birth, when the muscle hypertrophy phenotype is not expressed; and at 12 weeks of age, when the phenotype is fully apparent. It was concluded that changes in the postnatal developmental expression pattern of Dlk-1 are closely associated with the expression of the phenotype and that the callipyge mutation may promote a fetal-like gene expression program for some genes during postnatal life.
Additional keywords: Dlk-1, GTL2 , PEG11, MEG8.
Acknowledgments
We thank Jason White (University of Western Australia) and Matt McDonagh (Department of Primary Industries, Victoria) for aid in the collection of skeletal muscle samples from sheep. We are also grateful to Tracy Shay Hadfield, Dave Forrester and Sandy Eng (Utah State University) for assistance in sample collection and for providing access to a flock of sheep carrying the callipyge mutation. We also thank Lisa Leeton and Manuela Stolic for stimulating discussions. The research was supported by funds from Meat and Livestock Australia and Australian Wool Innovation through their Sheep Genomics Program. We are also grateful to an anonymous reviewer for insightful comments regarding the measurement of PEG11 and PEG11AS expression.
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