Breeding for Papaya ringspot virus resistance in Carica papaya via hybridisation with Vasconcellea quercifolia
R. A. Drew A C , S. V. Siar B , C. M. O’Brien A , P. M. Magdalita B and A. G. C. Sajise BA Faculty of Science, Griffith University, Nathan, Qld 4111, Australia.
B Institute for Plant Breeding, College of Agriculture, University of the Philippines at Los Baños, Laguna 4031, Philippines.
C Corresponding author. Email: r.drew@griffith.edu.au
Australian Journal of Experimental Agriculture 46(3) 413-418 https://doi.org/10.1071/EA04247
Submitted: 18 November 2004 Accepted: 11 August 2005 Published: 28 March 2006
Abstract
Papaya ringspot virus type P (PRSV-P) is a major threat to the papaya industry worldwide. F1 hybrids have been produced when Carica papaya L. female flowers have been pollinated with pollen of the PRSV-P resistant species Vasconcellea quercifolia. F1 plant production required embryo rescue 90 days post-pollination, and plantlet regeneration in vitro. Three hundred F1 hybrids were grown to maturity in the field and had morphological characteristics that were identical to 1 or both parents, were intermediate between those of the parents, or were greater than either parent. They had a sex ratio of 2 (male) : 49 (hermaphrodite) : 49(female). Eighteen plants (7 male and 11 hermaphrodite) produced some viable pollen. Pollen viability of these plants as measured by germination on agar medium varied between 1.1 and 6.1% (mean of 3.37 ± 0.18) compared with >90% for C. papaya. Cytogenetic studies showed limited homology between C. papaya and V. quercifolia genomes. The intergeneric hybrids varied in their reaction to strains of PRSV-P in Australia and the Philippines. Of the hybrids, 75% were resistant to PRSV-P while 25% produced virus symptoms.
Additional keywords: embryo rescue, in vitro culture.
Acknowledgments
The authors thank the Australian Centre for International Agricultural Research, the Australian Papaw Industry via Queensland Fruit and Vegetable Growers, and Horticulture Australia for funding this research.
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