An evaluation of potential Rhizobium inoculant strains used for pulse production in acidic soils of south-east Australia
J. EvansNSW Agriculture, Agricultural Institute, Private Bag, Wagga Wagga, NSW 2650, Australia. Email: jeffrey.evans@agric.nsw.gov.au
Australian Journal of Experimental Agriculture 45(3) 257-268 https://doi.org/10.1071/EA03129
Submitted: 24 June 2003 Accepted: 25 November 2003 Published: 14 April 2005
Abstract
Profitability of the pulse industry relies considerably on crop nitrogen fixation because this process supplies greater than 60% of pulse crop nitrogen. Therefore the industry requires the most efficient Rhizobium symbioses and effective inoculation management. Re-appraisal of the recommended inoculant strain for field pea, SU303, in south-east Australia, was warranted by field evidence that SU303 failed to maximise grain yield at sites in Western Australia. Re-appraisal of the inoculant strain for faba bean and lentil, WSM1274, was warranted because of anecdotal evidence from Western Australia of associated crop failures. In addition, a glasshouse study in Western Australia reported greater dry matter production by faba bean and lentil inoculated with strains other than WSM1274. This paper reports trials comparing potential inoculant strains for field pea and faba bean in soils of south-east Australia. Comparisons are based on efficiency for nitrogen fixation, survival on seed and survival in soil. Additionally, because the pulse industry lacked comprehensive information to assist decision making on the need for recurring inoculation, relevant investigation of this issue is also reported.
The results of 3 field experiments for efficiency for nitrogen fixation, over mildly (pHCa 5.0) to strongly (pHCa 4.3) acidic soil in south-east Australia supported replacing SU303 as the commercial inoculant. The efficiency for nitrogen fixation of WSM1274 on faba bean was not found to be inferior to alternative strains. However, its capacity for survival on seed at temperatures of 15°C and above, over a wide range of relative humidity, and perhaps its capacity for survival in acidic soil, was inferior. This provided additional evidence to justify the replacement of this inoculant strain that was agreed to by a national steering committee in 2001, based on the Western Australia reports, the early experiments in this study and those of a collaborative study in Victoria. Alternative inoculant strains to SU303 and WSM1274 were identified in the current study. Temperature and relative humidity conditions suitable for maintaining inoculant viability with extended storage of inoculated field pea and faba bean are also discussed.
A survey of rhizobia surviving in soil was used to determine the time scale of persistence of Rhizobium leguminosarum bv. viciae and Bradyrhizobium sp. (Lupinus) in soils of the south-east. It was concluded that in soils of pH (CaCl2) <5.1, inoculation of field pea and faba bean should be routinely practiced; none of the strains of R. leguminosarum bv. viciae tested showed ability for survival in strongly acidic soil sufficient to obviate seed inoculation. It was further concluded that the absence of a legume host for lupin rhizobia for 4 or more years would also warrant reintroducing inoculant of B. sp. (Lupinus).
Additional keywords: grain legume, nitrogen fixation.
Acknowledgments
I thank the Grains Research and Development Corporation for their financial assistance, Ms Jo Slattery and Mr David Pearce (Department of Primary Industries, Rutherglen) for provision of inoculant strains, Mr David Pearce and Mr Andrew Price (NSW Agriculture, Wagga Wagga) for technical assistance, and NSW Agriculture for provision of field sites and laboratory facilities.
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