An investigation into the effects of closure type, light and pH on the development of shoots and roots and subsequent establishment of micropropagated cauliflower (Brassica oleracea var. botrytis) plants
C. Tsipouridis A , T. Thomidis A B and A. Isaakidis AA Pomology Institute, Naoussa, P. C. 59200, Naoussa, Greece.
B Corresponding author. Email: thomi-1@otenet.gr
Australian Journal of Experimental Agriculture 45(10) 1331-1334 https://doi.org/10.1071/EA03068
Submitted: 20 March 2003 Accepted: 13 April 2005 Published: 15 November 2005
Abstract
A cauliflower micropropagation protocol is described with emphasis on the environmental conditions used. The environmental conditions within the culture jars were modified by using 4 different cover methods. The best results were produced in cultures covered with cotton wool, and the worst results with tightly-closed aluminium caps. Light intensity also produced significant differences between cultured transplants. The dry and fresh weights were about 50 and 28% higher, respectively, in cultures grown under light. In contrast, cultures grown under dark had about 20% higher water content. Results also showed the most acidic medium used (pH 4.5) was the best as an initiation medium.
Additional keywords: covers, light, pH, tissue culture.
Abe M,
Kanechi M,
Inagaki N, Maekawa S
(1997) Effects of the natural ventilation and light intensity on the growth, photosynthesis and acclimation of in vitro cultured cauliflower plantlets under photomixotrophic conditions. Environment Control in Biology 35, 47–53.
Crisp P, Gray AR
(1979) Successful selection for curd quality in cauliflower using tissue culture. Horticultural Research 19, 49–53.
Grout BW, Aston M
(1977) Transplanting of cauliflower plants regenerated from meristem culture. I. Water loss and water transfer related to changes in leaf wax and to xylem regeneration. Horticultural Research 17, 1–7.
Grout BWW, Crisp P
(1977) Practical aspect of the propagation of cauliflower by meristem culture. Acta Horticulturae 78, 289–296.
John CK, Nadgauda RS
(1999) In vitro-induced flowering in bamboos. In Vitro Cellular and Developmental Biology. Plant 35, 309–315.
Kumar VA,
Kumar A, Kumar J
(1992) In vitro regeneration of cauliflower (Brassica oleraceae convar botrytis var botrytis) from mature curd. Indian Journal of Agricultural Science 62, 429–431.
Kumar A,
Kumar VA, Kumar J
(1993) Rapid in vitro propagation of cauliflower. Plant Science 90, 175–178.
| Crossref | GoogleScholarGoogle Scholar |
Lazzeri PA, Dunwell JM
(1986) In vitro regeneration from seedling organs of Brassica oleracea var. italica Plenck cv. Green Comet. II. Effect of light conditions and explant size. Annals of Botany 58, 699–710.
Murashige T
(1974) Plant propagation through tissue cultures. Annals Review of Plant Physiology 25, 135–166.
| Crossref | GoogleScholarGoogle Scholar |
Plerik RLM, Steegmans HM
(1975) Effect of auxins, cytokinins, absisic acid and ethephon on regeneration and growth of bulbets on excised bulb scale segments of hyacinth. Physiologia Plantarum 34, 14–17.
Zhao D,
Xing J,
Li M,
Lu D, Zhao Q
(2001) Optimization of growth and jaceosidin production in callus and cell suspension cultures of Saussurea medusa. Plant Cell, Tissue and Organ Culture 67, 227–234.
| Crossref | GoogleScholarGoogle Scholar |
