Immunochemical characterisation of a methionine-rich protein from peanut
H. Mazhar A B and S. M. Basha AA Plant Biotechnology Laboratory, Florida A&M University, 301 Perry Paige, Tallahassee, Florida 32307, USA.
B Corresponding author. Email: Hifza.Mazhar@famu.edu
Australian Journal of Experimental Agriculture 44(12) 1221-1224 https://doi.org/10.1071/EA03061
Submitted: 10 March 2003 Accepted: 26 December 2003 Published: 24 January 2005
Abstract
Peanuts (Arachis hypogeae L. cv. Florunner), like other legumes, are low in sulfur-containing amino acids such as methionine and cysteine. Previous research from our laboratory has resulted in the identification and isolation of a methionine-rich protein (MRP) from peanut. To determine the differential deposition pattern of MRP during seed development and its breakdown pattern during seed germination, immunological studies were conducted using antibodies raised against MRP. The results showed that MRP-3 was deposited very early (in the ‘white’ maturity stage), while the MRP-5 accumulated at a later (the ‘yellow’ maturity stage) stage of seed development. Germination studies showed that MRP-3 degraded after 8 days of germination, while MRP-5 degraded after 10 days of germination. The MRP-3 antibody detected homologous proteins in several other legumes and cereals such as pea, pigeon pea, chick pea, red lentil, brown lentil, rice and wheat, while the MRP-5 antibody reacted with extracts from pea, chick pea and French bean, thus showing a conservation of the MRP subunits across species.
Additional keywords: immunoblotting, SDS–PAGE.
Acknowledgments
This research was supported by a grant from the USDA-CSREES, Washington DC. We thank Mike Ying and Rani Dhanarajan for their help with antibody production.
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