A novel 22-bp InDel in the intron 1 of the IGF1 gene is associated with slaughtering performance of Chinese Jiaji duck
Fanghu Wu
A , Lihong Gu B , Yuanyuan Shang A , Xiaohui Zhang A * , Zixin Xu C and Tieshan Xu D
A
B
C
D
Abstract
Insertion/deletion can affect poultry growth traits and slaughter performance. Poultry performance can be progressively improved by selecting poultry individuals carrying favorable variants for breeding.
The aim of this study was to analyze the correlation between insertion/deletion (InDel) polymorphisms in insulin-like growth factor 1 (IGF1) gene and slaughter performance and growth traits of Jiaji ducks.
We measured the growth traits and slaughter performance of 102 Jiaji ducks randomly selected at 90 days of age. The polymerase chain reaction products of Jiaji duck were genotyped using 2% agarose gel electrophoresis. One 22-bp InDel variant was found in the intron 1 of the IGF1 gene. General linear-model (GLM) procedures were used to determine the growth traits and slaughter performances with different InDel genotypes.
This 22-bp InDel variant exerted a significant influence on pre-slaughter bodyweight, carcass weight, eviscerated weight, percentage of skin fat, percentage of lean meat and percentage of leg muscle. The 22-bp InDel was adjacent to the transcription start site of the IGF1 gene and predicted to be nearby the core promoter of the IGF1 gene. The InDel mutation resulted in the loss of four transcription factor binding sites. The expression level of IGF1 mRNA was significantly reduced in the breast muscle of mutant individuals.
The results showed that the 22-bp InDel of the IGF1 gene had a significant effect on the slaughtering performance. Among the growth traits, the bodyweight showed significant effects, whereas no significant impacts were observed on other traits.
The InDel locus of the IGF1 gene can be used as a useful molecular marker for genetic breeding of Jiaji ducks.
Keywords: duck, genotypes, growth traits, IGF1 gene, insertion/deletion, meat quality, slaughter performance, transcription factor.
References
Annunziata M, Granata R, Ghigo E (2011) The IGF system. Acta Diabetologica 48, 1-9.
| Crossref | Google Scholar | PubMed |
Baeza E, Dessay C, Wacrenier N, Marche G, Listrat A (2002) Effect of selection for improved body weight and composition on muscle and meat characteristics in Muscovy duck. British Poultry Science 43, 560-568.
| Crossref | Google Scholar | PubMed |
Ding N, Tian D, Li X, Zhang Z, Tian F, Liu S, Han B, Liu D, Zhao K (2022) Genetic polymorphisms of IGF1 and IGF1R genes and their effects on growth traits in Hulun Buir Sheep. Genes 13, 666.
| Crossref | Google Scholar |
Gu L, Xu T, Liu Z, Lin D, Yang S, Ye B, Xing M, Lin Z (2018) Measurement and correlation analysis of growth performance of Jiaji duck. Heilongjiang Animal Science and Veterinary Medicine 89-93.
| Crossref | Google Scholar |
Gu L, Wang F, Lin Z, Xu T, Lin D, Xing M, Yang S, Chao Z, Ye B, Lin P, Hui C, Lu L, Hou S (2020) Genetic characteristics of Jiaji Duck by whole genome re-sequencing. PLoS ONE 15, e0228964.
| Crossref | Google Scholar |
Heldenbrand JR, Baheti S, Bockol MA, Drucker TM, Hart SN, Hudson ME, Iyer RK, Kalmbach MT, Kendig KI, Klee EW, Mattson NR, Wieben ED, Wiepert M, Wildman DE, Mainzer LS (2019) Recommendations for performance optimizations when using GATK3.8 and GATK4. BMC Bioinformatics 20(1), 557.
| Crossref | Google Scholar |
Hennebry A, Oldham J, Shavlakadze T, Grounds MD, Sheard P, Fiorotto ML, Falconer S, Smith HK, Berry C, Jeanplong F, Bracegirdle J, Matthews K, Nicholas G, Senna-Salerno M, Watson T, McMahon CD (2017) IGF1 stimulates greater muscle hypertrophy in the absence of myostatin in male mice. Journal of Endocrinology 234, 187-200.
| Crossref | Google Scholar |
Hou S, Liu L (2024) Report on the development of waterfowl industry and technology in 2023. Chinese Journal of Animal Science 60, 318-321.
| Google Scholar |
Kader Esen V, Esen S (2023) Association of the IGF1 5′UTR polymorphism in meat-type sheep breeds considering growth, body size, slaughter, and meat quality traits in Turkey. Veterinary Sciences 10, 270.
| Crossref | Google Scholar |
Lai Z, Wu F, Li M, Bai F, Gao Y, Yu J, Li H, Lei C, Dang R (2021) Tissue expression profile, polymorphism of IGF1 gene and its effect on body size traits of Dezhou donkey. Gene 766, 145118.
| Crossref | Google Scholar |
Li X, Qiu J, Liu H, Deng Y, Hu S, Hu J, Wang Y, Wang J (2020) MicroRNA-33a negatively regulates myoblast proliferation by targeting IGF1, follistatin and cyclin D1. Bioscience Reports 40, BSR20191327.
| Crossref | Google Scholar |
Li T, Qin P, Chen B, Niu X, Wang Y, Niu Y, Wei C, Hou D, Ma H, Han R, Li H, Liu X, Kang X, Li Z (2022) A novel 27-bp indel in the intron region of the YBX3 gene is associated with growth traits in chickens. British Poultry Science 63, 590-596.
| Crossref | Google Scholar | PubMed |
Liu H, Li X, Sun L, Wang H, Zhang R, Yang C, Li L, Wang J, He H, Krumm C (2014) Effects of the regulation of follistatin mRNA expression by IGF-1 in duck (Anas platyrhynchos) skeletal muscle. Growth Hormone & IGF Research 24, 35-41.
| Crossref | Google Scholar | PubMed |
Naicy T, Venkatachalapathy RT, Aravindakshan TV, Kurian E (2017) Association of a Cac8I polymorphism in the IGF1 gene with growth traits in Indian goats. Journal of Genetic Engineering and Biotechnology 15, 7-11.
| Crossref | Google Scholar |
Qin P, Liu Y, Niu X, Liu Y, Zhang Y, Niu Y, Wang Y, Chen B, Han R, Tian Y, Liu X, Kang X, Jiang R, Li Z (2023) Molecular characterization, expression profile, and A 21-bp Indel within the ASB9 Gene and its associations with chicken production traits. Genes 14, 339.
| Crossref | Google Scholar |
Robinson JT, Thorvaldsdóttir H, Winckler W, Guttman M, Lander ES, Getz G, Mesirov JP (2011) Integrative Genomics Viewer. Nature Biotechnology 29, 24-26.
| Google Scholar |
Talebi R, Ghaffari MR, Zeinalabedini M, Abdoli R, Mardi M (2022) Genetic basis of muscle-related traits in sheep: a review. Animal Genetics 53, 723-739.
| Crossref | Google Scholar | PubMed |
Wang WJ, Guo YQ, Xie KJ, Li YD, Li ZW, Wang N, Xiao F, Guo HS, Li H, Wang SZ (2021) A functional variant in the promoter region of IGF1 gene is associated with chicken abdominal fat deposition. Domestic Animal Endocrinology 75, 106584.
| Crossref | Google Scholar |
Weeks KL, Bernardo BC, Ooi JYY, Patterson NL, Mcmullen JR (2017) The IGF1-PI3K-Akt signaling pathway in mediating exercise-induced cardiac hypertrophy and protection. In ‘Exercise for cardiovascular disease prevention and treatment, Vol. 1000’. Advances in Experimental Medicine and Biology. (Ed. J Xiao) pp. 187–210. (Springer)
Werner H (2023) The IGF1 signaling pathway: from basic concepts to therapeutic opportunities. International Journal of Molecular Sciences 24, 14882.
| Crossref | Google Scholar |
Xu T, Gu L, Sun W, Yin J, Lin P (2020) The impact of different feeding modes on the production performance of Jiaji duck from 15 to 90 days of age. China Poultry 42, 61-65.
| Google Scholar |
Xu C, Gu L, Wu H, Wang Y, Yang S, Xu T (2023) The correlation analysis between body size and body weight of Jiaji duck. Heilongjiang Animal Science and Veterinary Medicine 10, 52-55.
| Crossref | Google Scholar |
Yoshida T, Delafontaine P (2020) Mechanisms of IGF-1-mediated regulation of skeletal muscle hypertrophy and atrophy. Cells 9, 1970.
| Crossref | Google Scholar |
Zhang X, Qi Y, Pang Y, Yuan B, Li X (2023) Identification of polymorphisms in the HERC2-OCA2 gene locus and their association with feather color in quail. The Journal of Poultry Science 60, 2023013.
| Crossref | Google Scholar |
